34results about How to "Early forecast" patented technology

The invention discloses a method for early and accurate prediction of rice bacterial blight by using miRNA162b gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA162b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA162b gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA162b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for forecasting bacterial leaf blight of paddy rice by utilizing miRNA396c gene at early stage, and belongs to the technical field of biology. The method comprises the following steps: selecting to-be tested paddy rice and control paddy rice, wherein the selected control paddy rice is paddy rice which is not infected by bacterial leaf blight and is cultivated under same conditions with the to-be tested paddy rice; respectively separating total miRNA gene of the to-be tested paddy rice and the control paddy rice; respectively detecting the expression level of miRNA396c gene in the total miRNA gene of the to-be tested paddy rice and the control paddy rice, wherein the sequence of the miRNA396c gene is shown as SEQ ID NO: 1; and determining whether the experiment is successful according to the expression level of miRNA396c gene in the to-be tested paddy rice and the control paddy rice, if successful, further determining whether the to-be tested plant is infected by bacterial leaf blight. The provided prediction method is successful, is capable of accurately performing forecast several days before paddy rice has symptoms of bacterial leaf blight, wins time for early prevention and early controlling, and helps to reduce loss of paddy rice caused by bacterial leaf blight.

The invention relates to an easily-occurring disease prediction system based on a merdian-collateral energy balance value, which comprises a main and collateral channels acquisition module, a pretreatment module, an easily-occurring disease prediction module, a prediction result processing module and a client terminal, wherein the main and collateral channels acquisition module acquires and analyzes to obtain the energy balance value of twelve main and collateral channels, and transmit to the preprocessing module. The preprocessing module comprises an energy area dividing unit, a merdian-collateral risk pattern map unit and a storage unit, wherein a merdian-collateral risk pattern map table established by the merdian-collateral risk pattern map unit is used for determining a risk pattern and a risk grade of each main and collateral channels, the storage unit assigns a value to the merdian-collateral risk prediction function fi to form a merdian-collateral risk mode set psi; the easily-occurring disease prediction module establishes a easily-occurring symptom prediction model to form a symptom set phi'; a easily-occurring disease prediction model is established to obtain a prediction function value Ri for the easily-occurring diseases in a disease set omega one by one; the prediction result processing module screens and sorts Ri to form a new easily-occurring disease set omega',and a data report is generated in the client terminal.

The invention discloses a method for predicting rice bacterial blight by using miRNA408 gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA408 gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA408 gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA408 gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for predicting rice bacterial blight by using miRNA395k gene, which belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA395k gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA395k gene is as shown in SEQ ID NO:1 in the sequence listing According to the expression levels of the miRNA395k gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention relates to a method and system for predicting the social network information transmission trend. The method includes the steps that information data and user date in a social network are obtained, and a user categorical distribution vector is calculated by means of the user data; normalization processing is carried out on the information data; smoothing processing is conducted on the normalized information data through a moving average method, a Diffusion-Info user categorical distribution vector is combined to figure out predicted points of the information transmission trend, and an information transmission trend line is drawn; a plurality of basic information transmission curves obtained through a K-SC algorithm and the information transmission trend line are fitted to obtain a follow-up trend line of the information transmission trend prediction line. According to the method and system, information transmission capacity can be estimated as soon as possible, hysteresis of a traditional method is reduced, and assistance is provided for timely pushing of information and timely control of public opinions of the social network; meanwhile, when the system operates, cost of an internal storage is low, and high efficiency, independence and transportability are achieved.

The invention discloses a method for predicting rice bacterial blight by using miRNA166b gene, which belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA166b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA166b gene is as shown in SEQ ID NO:1 in the sequence listing According to the expression levels of the miRNA166b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for predicting rice bacterial blight by using the expression of miRNA172b gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA172b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA172b gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA172b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for predicting rice bacterial blight with miRNA393a gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA393a gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA393a gene is as shown in SEQ ID NO:1 in the sequence listing According to the expression levels of the miRNA393a gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for predicting rice bacterial blight by using miRNA827 gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA827 gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA827 gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA827 gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for predicting rice bacterial blight by using miRNA5794 gene, which belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA5794 gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA5794 gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA5794 gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses a method for early and accurate prediction of rice bacterial blight by using miRNA398b gene, which belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is cultivated under the same conditions as the rice to be tested and not infected with bacterial blight; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in control rice; respectively detect the expression level of miRNA398b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA398b gene is as shown in SEQ ID NO:1 in the sequence listing ; According to the expression levels of the miRNA398b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.