Prediction of Rice Bacterial Blight Using miRNA399j Gene

Abstract

The invention discloses a method for predicting rice bacterial blight by using miRNA399j gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA399j gene in the total miRNA gene in the test rice and the control rice, the sequence of the miRNA399j gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA399j gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for predicting rice bacterial blight using miRNA399j gene. Background technique [0002] Rice is the main food crop in my country. Bacterial blight is one of the two major diseases of rice. It is a worldwide disease, especially in Asian rice regions. The incidence of bacterial blight is high and the infection is fast, and the rice fields with the disease will reduce production by 20-30%, or even 50%. Like most diseases, the early control effect of bacterial blight is better. In the middle and late stages of the disease, the pathogens have multiplied in large numbers, causing damage to the rice, and the control effect is poor. It can be seen that early forecasting is the key to the prevention of rice bacterial blight. [0003] The traditional rice bacterial blight forecast is mainly based on climate, weather, variety resistance, nitrogen fertilizer application, and disease...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the shortcomings of untimely and inaccurate forecasting of rice bacterial blight in the prior art, the embodiment of the present invention provides a method of using miRNA399j gene to predict rice bacterial blight

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