Method for forecasting bacterial leaf blight of paddy rice by utilizing miRNA396c gene

Abstract

The invention discloses a method for forecasting bacterial leaf blight of paddy rice by utilizing miRNA396c gene at early stage, and belongs to the technical field of biology. The method comprises the following steps: selecting to-be tested paddy rice and control paddy rice, wherein the selected control paddy rice is paddy rice which is not infected by bacterial leaf blight and is cultivated under same conditions with the to-be tested paddy rice; respectively separating total miRNA gene of the to-be tested paddy rice and the control paddy rice; respectively detecting the expression level of miRNA396c gene in the total miRNA gene of the to-be tested paddy rice and the control paddy rice, wherein the sequence of the miRNA396c gene is shown as SEQ ID NO: 1; and determining whether the experiment is successful according to the expression level of miRNA396c gene in the to-be tested paddy rice and the control paddy rice, if successful, further determining whether the to-be tested plant is infected by bacterial leaf blight. The provided prediction method is successful, is capable of accurately performing forecast several days before paddy rice has symptoms of bacterial leaf blight, wins time for early prevention and early controlling, and helps to reduce loss of paddy rice caused by bacterial leaf blight.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for forecasting rice bacterial blight by using miRNA396c gene. Background technique [0002] Rice is the main food crop in my country. Bacterial blight is one of the two main diseases of rice. It is a worldwide disease, especially in Asian rice regions. Bacterial blight has a high incidence and rapid transmission, and the yield of the diseased rice field is reduced by 20-30%, or even 50%. Like most diseases, the early control effect of bacterial blight is better. In the middle and late stages of the disease, the germs have multiplied in large numbers and have caused damage to the rice. The control effect is poor. This shows that early forecast is the key to the prevention of rice bacterial blight. [0003] Traditional rice bacterial blight forecasts are mainly based on climate, weather, variety resistance, nitrogen fertilizer application, and disease history. It can also...

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Problems solved by technology

[0006] In order to solve the shortcomings of untimely and inaccurate forecasting of rice bacterial blight in the prior art, the embodiment of the present invention provides a method of using miRNA396c gene to predict rice bacterial blight

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