7 results about "RNA amplification" patented technology

Synthetic endogenous ribonucleic acid virus satellite RNA molecules and satellite particles containing the same are disclosed. A synthetic endogenous ribonucleic acid virus satellite RNA molecule containing an endogenous heterologous TNA virus (HRV) amplicon is also disclosed. Also disclosed are methods of using endogenous ribonucleic acid virus satellite RNA molecules and satellite particles containing the same to alter plant phenotypes, improve stress resistance in plants, and improve pest and pathogen resistance in plants.

Disclosed in the present invention are a digital droplet RNA amplification detection system for prostate cancer and a method for reducing the false negative rate of prostate cancer. The provided digital droplet RNA amplification detection system for prostate cancer comprises reagents for respectively and specifically detecting the following genes on the basis of a real-time fluorescent nucleic acid constant-temperature amplification method: PCA3, ERG and SPDEF, and a digital PCR system.

A method for sample preparation and / or DNA amplification from urine, saliva and / or mouthwash samples is disclosed. The method involves providing a sample preparation system comprising a filter and providing a urine, saliva and / or mouthwash sample, then filtering at least a portion of the sample through the filter, thereby retaining bacteria, fungi, viruses protists and / or cancerous or precancerous cells in the filter. Subsequently a lysis reagent is applied to the filter. Finally, the DNA found in the lysate is amplified.

Synthetic split viral satellite RNA molecules and satellite particles containing the same are disclosed. Also disclosed are synthetic split viral satellite RNA molecules containing an internal heterologous TNA virus (HRV) amplicon. Also disclosed are methods of using the split viral satellite RNA molecules and satellite particles containing the same to alter plant phenotypes, improve stress resistance in plants, and improve pest and pathogen resistance in plants.

The application discloses a recombinant Bst DNA polymerase with high DNA polymerase activity and reverse transcriptase activity, and preparation and application thereof. Sulfolobus tokodaii The application carries out sequence rearrangement on a Headpiece domain truncated body Hp47 derived from chicken villin and a Sto7d domain derived from a thermophilic archaea, and fuses the amino terminal end of the Bst DNA polymerase large fragment to construct a novel Bst DNA polymerase rearrangement mutant HpSt-G23. The recombinant polymerase HpSt-G23 obtained by the application has high polymerase activity and reverse transcriptase activity, and can resist inhibitors such as urea and sodium chloride. Tth The HpSt-G23 can be used in a RecA detection system, and only two primers are needed to complete DNA or RNA amplification in the same system, and the detection sensitivity of the RNA template is greatly improved, so that the HpSt-G23 has a good development prospect in the field of nucleic acid detection.

The invention belongs to the technical field of molecular biological detection, and discloses a reaction system, a kit and a detection method applied to RNA amplification of a complex whole blood sample. The reaction system comprises a buffer component and an enzyme protection component; the buffer component consists of Tris and Bicine and is used for cooperatively maintaining the stability of the pH value of a reaction system in the RNA amplification process of a complex whole blood sample; and the enzyme protection component is composed of AEBSF and dextran sulfate. Through collaborative innovation of a double-buffer system and an active enzyme protective agent, the integrated challenges that the pH is easy to drift, the template compatibility is poor and the enzyme activity is easy to damage when a complex whole blood sample is faced in the prior art are effectively overcome, and a key technical support is provided for rapid, stable and reliable bedside molecular diagnosis.

Provided are a digital microdroplet RNA amplification detection system and method for reducing the missed detection rate of prostate cancer, which belong to the technical field of tumor diagnosis. The provided digital microdroplet RNA amplification detection system for reducing the missed detection rate of prostate cancer comprises: reagents based on a real-time fluorescent nucleic acid isothermal amplification method for specifically detecting the following genes, respectively: PCA3, ERG, and SPDEF; and a digital PCR system. Compared with existing kits or other products for prostate cancer detection, the provided digital microdroplet RNA amplification detection system can significantly reduce the missed detection rate of prostate cancer and improve the detection sensitivity and negative predictive value for prostate cancer.