Application of a dual-gene microRNA fluorescence quantitative internal reference and its primers in shellfish tissue samples

A fluorescence quantitative and tissue sample technology, applied in the field of molecular biology, can solve the problems of high cost and unsuitability for miRNA function analysis, and achieve the effect of low cost, high sensitivity and improved accuracy
CN103740823BActive Publication Date: 2015-09-23INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2015-09-23

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Abstract

The invention relates to the field of biology, and particularly relates to application of dual-genetic microRNA (miRNA) fluorescent quantitative internal references and a primer thereof in shellfish tissue samples. Two genes (let-7 and miR-87) selected by the invention are selected according a strict internal reference screening process, and are miRNA genes with the most stable expression in the shellfish microRNA in different tissues; the expression stability of the genes in the tissue is superior to the common miRNA internal reference genes snRNA U6, snRNA U1 and 5.8S RNA. Two miRNAs are selected at random, and the dual-genetic miRNAs are taken as internal references to carry out fluorescence quantitative analysis in the tissue samples; the obtained expression result is highly identical to miRNA sequencing expression profile; the reliability and the stability of the dual genetic internal reference are proved.
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Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a double-gene miRNA quantitative internal reference and primers suitable for microRNA (miRNA) expression analysis in shellfish tissue samples. Background technique

[0002] Shellfish, belonging to the clambranch class (or bivalves) in the mollusk phylum, common species include oysters, mussels, clams, and razor clams. There are about 10,000 species in existence, 80% of which live in the ocean. Most species of shellfish are edible. Many shellfish are tender, delicious, nutritious and have high economic value. They are important aquaculture objects in the world.

[0003] MicroRNA is a kind of small molecule non-coding single-stranded RNA that widely exists in the biological world. It is about 21-24nt long. It is widely expressed in different tissues and organs and participates in various physiological activities of organisms. Because of its importance in organisms, scientists at ...

Claims

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