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Preparation of water-soluble luminous silver nano cluster with double-stranded DNA as template

A silver nano-cluster, water-soluble technology, applied in the field of preparation of water-soluble luminescent noble metal nano-clusters, can solve the problems of synthesizing luminescent nano-clusters and the like, and achieve the effects of high quantum yield and good biocompatibility

Inactive Publication Date: 2014-01-29
YANCHENG INST OF TECH
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Problems solved by technology

So far, there has been no study on the synthesis of luminescent nanoclusters using cytosine base-rich double-stranded DNA as a template

Method used

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  • Preparation of water-soluble luminous silver nano cluster with double-stranded DNA as template

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Embodiment Construction

[0012] Using the single-stranded DNA rich in cytosine bases and reserved with 6 loops of cytosine bases, by adding its complementary strand, after annealing, a double-stranded DNA with a loop ring is formed, which is used as a template, Add an equivalent amount of probe strand (probe ssDNA, 5'-CCCTAACCCTCCCCCCAACCCTAACCCTA-3', 2μM) and target strand (target ssDNA, 5'-TAGGGTTAGGGTTAGGGTTAGGG-3', 2μM) solution (the configuration method of the DNA solution is to mix an appropriate amount of DNA The dry powder is dissolved in the corresponding buffer solutions of different pH values), and after mixing evenly, it is placed in a 95-degree constant temperature water bath to make it denatured for 15 minutes; put it in the 0°C ice-water mixed bath for more than 5 minutes; add an appropriate volume of silver nitrate solution (100 μM), shake it for 15 minutes to make it evenly mixed; then, quickly add an appropriate volume of sodium borohydride solution (100 μM, prepared and used now), c...

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Abstract

Provided is preparation of a water-soluble luminous silver nano cluster with double-stranded DNA as a template. Firstly, single-stranded DNA rich in cytosine base group is used, 6 cytosine base group loops are reserved in the single-stranded DNA, the total number of the cytosine base groups is 29, single-stranded DNA rich in guanine base group complements the single-stranded DNA and is added, the total number of the guanine base groups is 23, the double-stranded DNA with the 6 cytosine base group loops is formed through the annealing function and is used as the template, and synthesis is achieved through the method of adding sodium borohydride to restore silver nitrate. The preparation process is simple, easy to implement and environmentally friendly, and the preparation can be applied to the preparation of other luminous nano materials. In addition, new nano materials synthesized through the method have water solubility, a high quantum yield, light stability, good biocompatibility and other performance, and have potential wide application to the fields of biosensors, cell imaging, clinical medicine and the like.

Description

technical field [0001] The invention relates to the field of preparation of water-soluble luminescent noble metal nano-clusters. Specifically, it relates to the field of synthesizing silver nanoclusters capable of emitting specific wavelengths in aqueous phase based on double-stranded DNA as a template. Background technique [0002] In the past decade, noble metal nanoclusters have been developed as a new type of fluorophore due to their strong, size-dependent fluorescence emission spectra [Participate in: Díez I., Ras R.A. Fluorescent silver nanoclusters. Nanoscale, 2011, 3, 1963-1970.]. In particular, the synthesis of water-soluble, biocompatible and luminescent silver nanoclusters using DNA as a template has attracted considerable attention [see: Petty J.T., Zheng J., Hud N.V., et al. DNA-templated Ag nanocluster formation. J. Am. Chem. Soc., 2004, 126, 5207-5212.]. Luminescent silver nanoclusters were synthesized using single-stranded DNA as a template, exhibiting rem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
Inventor 刘国良冯大千王伟
Owner YANCHENG INST OF TECH
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