The invention relates to a preparation method of a multi-tone beet red pigment, and the preparation method is characterized in that fresh red beet is adopted as a raw materials to be continuously produced through the procedures of washing, slicing, extraction, filtering, column chromatography, color protection, concentration, color matching, ultrafiltration, microcapsulization, atomizing drying and mixed treatment, a beet red pigment product is obtained by utilizing a method combining a staging elution process of macroporous resin with a particular model number, pure water with particular electric conductivity and the mixed antioxidant, the color value of the beet red pigment product is more than or equal to 80, color tones include orange red (b: 14 to 8), pink (b: 8 to 5), true red (b: 5 to 0) and amaranth (b: 0 to 10). The beet red pigment has characteristics of light resistance, heat resistance, stability and the like. The beet red pigment is better than similar product standard both at home and abroad.