The invention discloses a polyester-cotton interwoven one-way moisture-wicking yarn-dyed fabric and a dyeing and finishing processing method thereof, which are characterized in that the fabric is woven with warps and wefts, the warps are polyester filaments, the wefts are pure cotton yarns, and the dyeing and finishing processing method of the fabric comprises the steps of S1, carrying out warp finishing, to be specific, unwinding, dyeing, carrying out reduction washing, and post-treating; S2, carrying out weft finishing, to be specific, dyeing, removing a surfactant, pickling, carrying out waterproof treatment, dewatering, and drying; S3, for the warps and wefts from the steps S1 and S2, carrying warping, rewinding, sizing, weaving, and maintaining gray fabric and post-treating; a honeycomb structure is adopted during weaving, the wefts are visible on the skin-fitting surface, the warps are visible on the outer surface, and the structure is loose; the post-treating procedure includesbaking, washing, shaping, setting and stentering, and preshrinking. The polyester-cotton interwoven one-way moisture-wicking yarn-dyed fabric and the dyeing and finishing processing method thereof have advantages that a certain waterproof effect is given to the skin-fitting surface of the fabric, the external surface of the fabric has excellent moisture-wicking effect, and the adopted polyester filaments with cotton yarns also improve quick-drying effect, so that the moisture loss speed is higher.