The invention discloses a protective clothing material based on a spunlace non-woven material and a preparation method thereof. An outer protective layer, an adhesive layer and a spunlace non-woven fabric layer are sequentially attached, the spunlace non-woven fabric layer is subjected to water-repellent, oil-repellent, blood-resistant and alcohol-resistant four-antibody finishing, the outer protective layer is a PTFE film, and the spunlace non-woven fabric layer is made of bamboo pulp fibers and polyester fibers. The preparation method comprises the following steps: preparing the spunlace non-woven fabric layer, namely mixing bamboo pulp fibers and polyester fibers in proportion, carding, lapping, drafting, performing high-pressure spunlace, performing four-antibody finishing, drying at high temperature, sterilizing, slitting and rolling to obtain the spunlace non-woven fabric layer; wherein the surface of the spunlace non-woven fabric layer is coated with an adhesive, then the spunlace non-woven fabric layer is covered with a PTFE film, and the final material is obtained through multi-stage tentering shaping after pressurization compounding of a heating roller. The protective clothing material is free of any rolling points, soft in hand feeling, good in air permeability, comfortable to wear and good in protective property.