The invention belongs to the field of manual metalloenzyme and discloses high-efficiency decolorizing
peroxidase and a preparation method and application thereof. According to the decolorizing
peroxidase disclosed by the invention, myohemoglobin with an
oxygen carrying function is utilized as
protein molecules to design a framework,
tyrosine is introduced to a 43 site near the
heme active center of the myohemoglobin, and
tryptophan is introduced to a 138 site; meanwhile,
tryptophan is introduced to an 88 site at the periphery of the
heme active center to obtain three-
point mutant protein, andan
amino acid sequence of the three-
point mutant protein is shown as SEQ ID NO.1. According to the decolorizing
peroxidase disclosed by the invention, a catalytic conversion number is improved, and dependence on
hydrogen peroxide is reduced; the preparation method of the decolorizing peroxidase has the advantages of simpleness in operation and suitability for large-scale industrial production. Experiments show that the decolorizing peroxidase disclosed by the invention can degrade dye molecules under low-concentration
hydrogen peroxide and within several seconds to achieve a decolorizing effect, a
catalytic efficiency is increased by 130% compared with that of two-
point mutant protein, and the decolorizing peroxidase can be widely applied to
pollution treatment of industrial dye
wastewater.