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52results about How to "Guaranteed Affinity" patented technology

Heparin affinity column and preparation method and use thereof

The invention discloses a heparin affinity column as well as the preparation method and the application thereof. The heparin affinity column is prepared through the following method: agarose gel 6FF is taken as a solid phase carrier which is firstly activated and then is aminated and coupled with amidocyanogen after an epoxy group is coupled, the aminated agarose gel 6FF and the heparin form intermediate aldimine in methanol, and the intermediate product of aldimine is further subjected to reductive amination for forming stable chemical bonds, thereby acquiring the heparin-agarose gel 6FF. The affinity column preparing preparation with high efficiency is simple and short. Through adopting the combination of the rear aldehyde of the heparin and the amidocyanogen of aminated sepharose, the activity of the heparin is not influenced, thereby ensuring the affinity column to have higher affinity; the heparin coupling is stable, and the heparin can be repeatedly used; the reagent adopted by the method has low cost and no environmental pollution, thereby laying a foundation for sweepingly isolating and purifying materials with specific binding capacity to the heparin in common laboratories, and promoting the research on domestic relative fields.
Owner:NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A

Decolorizing peroxidase and preparation method and application thereof

The invention belongs to the field of manual metalloenzyme and discloses high-efficiency decolorizing peroxidase and a preparation method and application thereof. According to the decolorizing peroxidase disclosed by the invention, myohemoglobin with an oxygen carrying function is utilized as protein molecules to design a framework, tyrosine is introduced to a 43 site near the heme active center of the myohemoglobin, and tryptophan is introduced to a 138 site; meanwhile, tryptophan is introduced to an 88 site at the periphery of the heme active center to obtain three-point mutant protein, andan amino acid sequence of the three-point mutant protein is shown as SEQ ID NO.1. According to the decolorizing peroxidase disclosed by the invention, a catalytic conversion number is improved, and dependence on hydrogen peroxide is reduced; the preparation method of the decolorizing peroxidase has the advantages of simpleness in operation and suitability for large-scale industrial production. Experiments show that the decolorizing peroxidase disclosed by the invention can degrade dye molecules under low-concentration hydrogen peroxide and within several seconds to achieve a decolorizing effect, a catalytic efficiency is increased by 130% compared with that of two-point mutant protein, and the decolorizing peroxidase can be widely applied to pollution treatment of industrial dye wastewater.
Owner:NANHUA UNIV

Method for combining large-diameter tree/tree house with multiple small trees

The invention discloses a method for combining large-diameter tree / tree house with multiple small trees and provides a method for combining small tree seedlings with a ground diameter of phi (1cm-3cm) into one tree and growing into a tree with a huge diameter in 5-10 years. Moreover, another function of the tree is that the tree is a new variety not existing in the nature. The method for combining large-diameter tree / tree house with multiple small trees comprises the technical procedure as follows: small tree seedlings with a ground diameter of phi (around 1cm-3cm) and a height of 1.5-3m are selected; then a pit is dug and a plurality of small tree seedlings are together placed in the pit; then two planes of two rind parts of 60mm sections of the trunks of two small tree seedlings in the most center which can be contacted are selected and flatly jointed; then 2-4 small holes are drilled in two contact points; crabsticks with a ground diameter of phi (1mm) are inserted into the holes; then the residual crabsticks are removed so that the crabsticks are aligned to an outer wall; then the crabsticks are fixed by thin ropes; 502 or white latex glue is coated between the crabsticks and the hole walls so that the crabsticks and the hole walls are firmly stuck; after fixation, the rope is unfastened; and so forth, a neighboring tree is placed closely and connected by crabsticks; afterthe connection is completed, the trees are bound by a soft rope; then the soft rope is removed in a half year so that the trees can grow naturally; after the trees grow higher, the height positions are connected by adopting the same method. The trunks are outwards bent at a required height and are fixed by bending plates with a bending angle of 120-150 degrees (as shown in diagram 3); then the bending plates are removed in a half year; and the trees are conventionally and carefully cultured; the crown is pruned; and the trees grow into a tree with a huge diameter in a plurality of years.
Owner:朱永平

Cleansing water capable of repairing damaged sebum membrane and preparation method of cleansing water

InactiveCN105796384ARemove makeup thoroughlyNo greasy feelingCosmetic preparationsMake-upMoistureSqualene
The invention provides cleansing water capable of repairing the damaged sebum membrane. The cleansing water comprises basic ingredients and functional ingredients, and the balance of deionized water, wherein the basic ingredients comprise the following components in percentage by weight: 5-25% of a surfactant, 5-25% of a humectant, 0.02-0.16% of citric acid, 0.01-0.1% of sodium citrate, and 0.2-1% of phenoxyethanol. The functional ingredients adopt any one of the following two compositions: composition I: 2-6% of N-cetylhydroxyproline palmitamide, 0.2-2% of tocopheryl acetate, 0.2-2% of bisabolol, and 0.2-1% of plant essential oil; and composition II: 10-20% of shea butter and/or squalene and/or ceramide. The cleansing water contains various functional components, and the functional components do not take effects independently but act as a whole, and are matched for use; the contents of the components are optimized, so that the prepared cleansing water has the efficacies of repairing the damaged sebum membrane, removing all traces of make-up without residue, being free of stimulation and oily feel, and being capable of effectively preventing the skin aging, preserving the moisture and tendering the skin, and enabling the pores to be smooth.
Owner:CANTON DAMEKISS DAILY CHEM FACTORY LIMITED

High-tower compound fertilizer containing lignin and calcium lignosulphonate and preparation method thereof

The invention discloses a high-tower compound fertilizer containing lignin and calcium lignosulphonate, wherein the high-tower compound fertilizer comprises ammonium nitrate phosphate, Mannheim potassium sulfate, instant lignin and calcium lignosulphonate, diammonium phosphate, monoammonium phosphate and anhydrous magnesium sulfate; the instant lignin and calcium lignosulphonate are small molecules, and have the molecular weight of 20 kDa-200 kDa; a small amount of micromolecular instant lignin and calcium lignosulphonate can be organically combined with high-concentration nutrient elements, the slow release function and affinity are not affected by high temperature, and the obtained compound fertilizer is sufficient in nutrient and rich in high-concentration quick-acting nutrient elements, has the advantages of high activity, full water solubility, quick acting, excellent slow release performance and the like, and is efficient and convenient. The invention further discloses a preparation method of the high-tower compound fertilizer containing lignin and calcium lignosulphonate; the preparation process and parameters of the high-tower compound fertilizer are optimized, and the high-tower compound fertilizer is good in stability, easy to pelletize and form and high in activity of effective components.
Owner:广东瑞丰生态环境科技股份有限公司

Preparation method of modified polyurethane pervaporation phenol/water separation membrane

Belonging to the technical field of pervaporation membrane separation, the invention relates to a preparation method of a modified polyurethane pervaporation phenol / water separation membrane. The preparation method of the membrane consists of: reacting hydroxyl-terminated polybutadiene with diisocyanate to obtain a terminated prepolymer, adding diamine and cyclodextrin as a chain extender for chain extension, and taking N, N-dimethyl acetamide as a solvent to obtain a modified polyurethane solution, carrying out membrane casting on the solution on a polyfluortetraethylene plate, and conducting curing at room temperature for 1 to 1.5 hours, then moving the obtained membrane into an oven at a temperature of 80DEG C for 10-15 hours of heat treatment, thus obtaining the modified polyurethane membrane. The material synthesis and membrane preparation process of the invention is simple, and the prepared membrane has good thermal and chemical stability as well as mechanical properties. Moreover, compared with traditional unmodified polyurethane separation membranes, the membrane prepared in the invention, due to the existence of the modifier cyclodextrin, has a better comprehensive separation effect on phenol in a phenol / water mixed solution, and has a great practical value.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Low-smoke halogen-free fireproof cable

The invention provides a low-smoke halogen-free fireproof cable. The low-smoke halogen-free fireproof cable sequentially comprises a low-smoke halogen-free flame-retardant polyolefin layer, a non-woven fabric belt, a glass fiber belt, an insulating filling layer and a plurality of cable core units from outside to inside. The preparation method of the low-smoke halogen-free flame-retardant polyolefin layer comprises the following steps: stirring and mixing the BASF flame retardant, the 1, 3, 5-triazine, the pentaerythritol and the hindered phenol antioxidant in parts by weight; adding plant cellulose and magnetite nanoparticles into the obtained mixture, and fully stirring again; and adding polyolefin and a charring agent in parts by weight into the obtained mixture, and stirring and extruding by using a double-screw extruder to obtain the low-smoke halogen-free flame-retardant polyolefin layer. The low-smoke halogen-free fireproof cable has good flame retardant property, corrosion resistance and mechanical property, and reduces the release of toxic and harmful gases; and the plant cellulose and the magnetite nanoparticles are added to prepare the polyolefin layer so that the mechanical property, the antibacterial activity, the elasticity and the compression resistance of the cable can be improved.
Owner:湖南华菱线缆股份有限公司

Method for combining large-diameter tree/tree house with multiple small trees

The invention discloses a method for combining large-diameter tree / tree house with multiple small trees and provides a method for combining small tree seedlings with a ground diameter of phi (1cm-3cm) into one tree and growing into a tree with a huge diameter in 5-10 years. Moreover, another function of the tree is that the tree is a new variety not existing in the nature. The method for combining large-diameter tree / tree house with multiple small trees comprises the technical procedure as follows: small tree seedlings with a ground diameter of phi (around 1cm-3cm) and a height of 1.5-3m are selected; then a pit is dug and a plurality of small tree seedlings are together placed in the pit; then two planes of two rind parts of 60mm sections of the trunks of two small tree seedlings in the most center which can be contacted are selected and flatly jointed; then 2-4 small holes are drilled in two contact points; crabsticks with a ground diameter of phi (1mm) are inserted into the holes; then the residual crabsticks are removed so that the crabsticks are aligned to an outer wall; then the crabsticks are fixed by thin ropes; 502 or white latex glue is coated between the crabsticks and the hole walls so that the crabsticks and the hole walls are firmly stuck; after fixation, the rope is unfastened; and so forth, a neighboring tree is placed closely and connected by crabsticks; afterthe connection is completed, the trees are bound by a soft rope; then the soft rope is removed in a half year so that the trees can grow naturally; after the trees grow higher, the height positions are connected by adopting the same method. The trunks are outwards bent at a required height and are fixed by bending plates with a bending angle of 120-150 degrees (as shown in diagram 3); then the bending plates are removed in a half year; and the trees are conventionally and carefully cultured; the crown is pruned; and the trees grow into a tree with a huge diameter in a plurality of years.
Owner:朱永平
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