The invention belongs to the technical field of textile fabrics and relates to a manufacturing method of hemp/silkworm silk high-count blended knitted fabric. The manufacturing method comprises a line-forming technique and a cloth-forming technique, wherein the line-forming technique comprises the following steps: high-count blended yarn forming, triple singeing treatments, mercerization, degumming, dyeing, silk turning, spooling and the like of the hemp/silkworm silk; and the cloth-forming technique comprises the following steps: card nepremoval, humidifying, weaving, primary singeing treatment, mercerization, softening treatment, sizing, pre-shrinking, batching package and the like. The high-count blended knitted fabric has functions of antibacterium, bacteriostasis and ultraviolet radiation resistance, has good humidity-absorbing and air-permeable performances, high temperature resistance, softness and comfortableness, and can meet different requirements of crowds at all ages and all levels.