The invention relates to a nanofiber mouth mask capable of effectively blocking PM0.3 and rich in nanometer antibacterial ingredients and belongs to the technical field of daily protection products. The nanofiber mouth mask comprises a nanometer antibacterial layer, a first non-woven layer, a coarse effect filtering layer, an efficient filtering layer and a second noon-woven layer. The first non-woven layer is arranged on the outer side of the mouth mask, the second non-woven layer is arranged on the inner side of the mouth mask, the nanometer antibacterial layer is sprayed on the surface of the first non-woven layer, finally the five layers of structures are combined through an ultrasonic bonding method, either or both of a spunbond fabric and an SMS spunbond fabric can be selected for the first noon-woven layer and the second non-woven layer, a melt-blown fabric or spunlace or electrostatic cotton can be selected for the coarse effect filtering layer, the efficient filtering layer is prepared through an electrospinning technique, and the nanometer antibacterial layer is prepared in an electronic injection or coating mode. The mouth mask is high in capturing efficiency for fine particles, especially for PM 0.3 fine particles, low in breathing resistance and free of irritation to the skin in the using process, and the nanometer antibacterial layer can effectively inhibit or kill germs, bacteria and the like in the air.