31 results about "Dna nanostructure" patented technology

The invention relates to polymer nanoparticle and DNA nanostructure delivery compositions for non-viral delivery, and methods therefor. More particularly, the invention relates to polymer nanoparticle delivery compositions, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, and DNA nanostructure delivery compositions, such as DNA origami compositions, for the delivery of more than one payload, or for the delivery of a nucleic acid construct payload of 3 kB or more, and methods therefor.

The invention provides a DNA (Deoxyribose Nucleic Acid) nano-structure carrier. The DNA nano-structure carrier provided by the invention has an EGFR (epidermal growth factor receptor) aptamer structure and is used for loading a connecting sequence of siRNA; moreover, after the siRNA is loaded, the DNA nano-structure carrier can further load a chemotherapeutic drug such as adriamycin, so that a DNA nano-drug based on the DNA nano-structure carrier is obtained. Through the target gene expression knock-down effect of siME3, the cytotoxicity of adriamycin and the targeting property of the EGFR aptamer structure, the DNA nano-drug provided by the invention can effectively inhibit pancreatic cancer, especially the growth of ME2 deletion type pancreatic cancer cells.

This work proposes a novel enzyme-free electrochemiluminescence sensing platform based on novel DNA-quantum dot nanostructures and hybridization chain reaction (HCR) amplification, and applies it to trace Cd. 2+ The detection; first, Cd 2+ Aptamer-triggered HCR amplification introduces a large amount of biotin-labeled DNA into the electrode, whereby biotin specifically captures a large amount of avidin SA-CdS quantum dot complexes, exhibiting a high ECL signal; target Cd 2+ Upon binding to its aptamer, the quantum dot-DNA structure detaches from the electrode, resulting in a significant decrease in the ECL signal, thus enabling the control of Cd. 2+ This sensor exhibits ultrasensitive detection with a dynamic response range from 10 fM to 10 nM and a detection limit of 2.6 fM. It demonstrates excellent selectivity, speed, high sensitivity, and practicality for detecting real water samples, providing a novel and competitive strategy for detecting heavy metal ions in real-world samples.

The invention discloses a molecular system based on DNA nano-structure artificial atoms and a construction and regulation method, and belongs to the technical field of DNA nanotechnology. The construction method of the molecular system comprises the following steps: mixing DNA chains with extension chains, adding a TM buffer solution, and carrying out annealing reaction to synthesize a DNA nano-structure with extension chains; dNA nanostructures are used for representing artificial atoms, extended chains on the DNA nanostructures are used for representing valence electrons, double chains formed by interaction among the extended chains are used for representing chemical bonds, a plurality of DNA nanostructures with extended chains are mixed in proportion according to a target molecular configuration, and a molecular system is obtained through one-pot assembly. Dynamic dissociation and recombination of artificial atoms are achieved based on DNA strand displacement reaction, and accurate regulation and control of a programmable molecular system are achieved. The construction precision and regulation effectiveness of the molecular system are verified through various technologies, the system has the advantages of being high in programmability, high in assembly efficiency, good in structural controllability and the like, and a brand new technical platform is provided for the fields of material design, chemical process simulation, biological engineering and the like.

The invention belongs to the technical field of biomedicine and nano crossing, and particularly relates to a biomineralization-based nucleic acid aptamer functionalized DNA nano-structure compound as well as a preparation method and application thereof. The compound has a tetrahedral structure and comprises a functional DNA tetrahedral framework and a calcium phosphate coating coated on the surface of the functional DNA tetrahedral framework, the functionalized DNA tetrahedral framework is formed by self-assembly of four DNA single chains; wherein the 5'end of one DNA single chain is modified with a nucleic acid aptamer. The bionic calcium coating is used as a protective shell, so that the in-vivo stability and the angiogenesis promoting activity of the Apt02 are remarkably improved. Meanwhile, by virtue of the specific spatial configuration of the tetrahedral DNA nanostructure, the compound can release calcium ions after being taken by cells, so that osteogenic differentiation is further promoted, and a new strategy for synergistic promotion is provided for vascularized bone regeneration.

Provided herein, in some embodiments, are nucleic acid nanostructure-based vaccines.

Described herein is a DNA nanostructure-based platform that can modulate a number of cellular functions depending on the concentration and surface decoration of the nanostructure. Utilizing different peptides for surface functionalization of DNA nanostructures, lysosomal activity was able to be modulated which in turn translated into the control of cellular functionality, ranging from changes in cell morphology to modulation of immune signaling and cell death. This demonstrates the rational design of a new generation of versatile DNA-based nanoplatforms that can be used in various biomedical applications such as the development of combinatorial anti-cancer platforms, efficient systems for endolysosomal escape, and nanoplatforms modulating lysosomal pH.

The invention relates to a double DNA nanosphere probe (DDNS) as well as a preparation method and biosensing application thereof. The DDNS comprises a DNA (deoxyribonucleic acid) nanosphere 1 (DNS-1) and a DNA nanosphere 2 (DNS-2). The DNS-1 and the DNS-2 are respectively formed by self-assembling two cholesterol modified DNA single-stranded probes with hairpin structures, and are respectively used for identifying target miRNA and triggering a non-enzymatic circulating strand displacement amplification (T-SDA) reaction. The DDNS can realize high-sensitivity fluorescence detection on target miRNA and can effectively inhibit non-target miRNA interference, and the interference inhibition rate is close to 100%. Besides, DDNS has excellent nuclease stability, the system can automatically enter living cells without a transfection reagent, high signal-to-noise ratio fluorescence differentiation of cancer cells and healthy cells is realized on the premise of maintaining cell activity, the performance is equivalent to that of a transfection agent dependent system, and the biological safety is remarkably improved. The invention provides a new material and a new method for application of the DNA nanostructure in the fields of molecular diagnosis and precision medical treatment.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

The invention provides a pellicle drug-loading system and a preparation method and application thereof, the pellicle drug-loading system is of a structure that an outer membrane wraps a core, and the outer membrane is a bacterial outer membrane; the core is formed by loading near-infrared two-region AIE photo-thermal molecules on a tetrahedral DNA (deoxyribonucleic acid) nano structure. The invention has the advantages that: the mycoderm drug delivery system can realize spatial dual targeting and time visual regulation and control on pathogens, and constructs a positive feedback cycle of drug delivery-immune activation through triple function synergy, thereby providing a drug basis for treatment of bacterial infection.

The invention discloses a fermentation medium for efficiently producing single-stranded DNA and a DNA nanostructure by a phagocytosis method and application of the fermentation medium, and relates to the technical field of biology. The fermentation culture medium is obtained by using ammonium chloride as a single inorganic nitrogen source to replace a nitrogen source (NH4) 2HPO4 in a Riesenberg culture medium. The invention also provides a method for efficiently producing single-stranded DNA (ssDNA) and a DNA nanostructure by a phagocytic grain method, high yield of the ssDNA can be promoted by adopting the fermentation culture medium, a nitrogen source is optimized and screened through the culture medium, and the invention finds that the yield of the single-stranded DNA can be increased by using ammonium chloride as a unique inorganic nitrogen source in the fermentation culture medium for production, and the yield of the single-stranded DNA can be increased by using the ammonium chloride as the unique inorganic nitrogen source in the fermentation culture medium. The yield of single-stranded DNA with triangular, hairpin rectangular, rod-like and quadrilateral DNA structures is increased to different extents by the fermentation culture medium, and a theoretical basis is provided for large-scale production of different types of ssDNA and DNA structures.

The application discloses a kind of space-time controllable T cell junction based on DNA nanostructure and its construction method and application, belong to DNA nanotechnology field.T cell junction includes six helix bundle paper structure and assembled internal functional layer and external shielding layer on it;Six helix bundle paper structure is assembled using 63 staple chain and M13;The internal functional layer includes nucleic acid-antibody conjugate combined on the four helix extension sequences of the top and bottom of the six helix bundle paper structure, and hand-in-hand palindromic sequence combined on the two helix extension sequences on left and right sides;External shielding layer includes C chain modified serum albumin binding peptide and chain I, and chain I includes pH response i-motif switch.T cell junction of the application has the space-time control ability of pH response and the potential of inducing TCR cluster to enhance T cell activation, has great potential to improve the safety and effectiveness of T cell immunotherapy.

The invention provides a DNA nano-structure connection method, system and product based on polymerase regulation and control, and belongs to the technical field of DNA paper-cut. The method comprises the following steps: respectively preparing a first DNA nano-structure and a second DNA nano-structure, a first connecting sequence in a first connecting chain on the first DNA nanostructure is complementary to a second connecting sequence in a second connecting chain on the second DNA nanostructure; mixing the first locked single strand with the first DNA nanostructure to form a first composite structure; mixing a second locked single strand with a second DNA nanostructure to form a second composite structure; placing the first composite structure and the second composite structure in the same reaction system; when connection of the first DNA nanostructure and the second DNA nanostructure needs to be achieved, primer chains and DNA polymerase are added into a reaction system, so that connection of the first DNA nanostructure and the second DNA nanostructure is completed through hybridization between complementary connecting chains.

The present invention relates to a composition for cryopreservation, comprising: a nucleic acid structure which comprises a scaffold nucleic acid folded at predetermined positions to form multiple strands, and a plurality of staple nucleic acids wherein at least a portion of a sequence thereof comprises a complementary sequence to that of the scaffold nucleic acid, which are bound to at least one of the strands of the scaffold nucleic acid to form a double strand; linkers coupled to at least one of single strands in the nucleic acid structure; and an anti-freezing peptide coupled to at least one of the linkers, so as to exhibit excellent freeze-protection effects, which in turn increase cell viability during cryopreservation of cells and tissues, while retaining original texture of food even when used for freezing the food.

The application discloses a DNA nano antisense drug targeting microRNA, a preparation method and application thereof. The DNA nano antisense drug comprises a DNA nanostructure as a carrier, antisense peptide nucleic acid and selected aptamer, the DNA nanostructure wraps the antisense peptide nucleic acid, and exposes the selected aptamer, the antisense peptide nucleic acid is connected to the 3' end of a staple chain constituting the DNA nanostructure, and the selected aptamer is modified to the 5' end extended from another staple chain constituting the DNA nanostructure, and the antisense peptide nucleic acid can specifically recognize target microRNA in tumor cells. The DNA nano antisense drug has the advantages of simple preparation process, low price and harmless metabolic products, has strong specific binding effect on target microRNA and can promote cell apoptosis, and has great application value in the field of anticancer drug preparation.

The invention discloses a silicon dioxide cluster as well as a preparation method and application thereof. The method comprises the following steps: in an alkaline buffer solution, carrying out a dehydration condensation reaction by controlling the molar concentration of TMAPS and TEOS, and carrying out a curing process to obtain the monodisperse and super-stable cationic silicon dioxide cluster. The cationic silicon dioxide cluster prepared by the method disclosed by the invention not only can be used for accurate assembly and mineralization reaction of a DNA nano-structure template and provides a new way for construction of a functional silicon-based material, but also realizes effective stability of an intermediate process before and after nucleation; the general mechanism of template-oriented natural and bionic amorphous mineralization can be disclosed, and the method has important significance for promoting the development of bionic silicon-based materials.

The invention relates to the technical field of image processing, in particular to a fluorescence image signal enhancement system and method based on a three-dimensional DNA nanostructure. The method comprises the following steps: carrying out image identification on a fluorescence image to determine a plurality of diffraction spot areas; in the plurality of diffraction spot regions, distinguishing a first region corresponding to a single-molecule diffraction spot and a second region corresponding to an overlapped diffraction spot; fitting the gray level distribution of the first area to obtain first fitting information; distinguishing a mixed sub-region and a pure sub-region in the second region, and fitting the gray distribution of the pure sub-region by taking the gray distribution of the mixed sub-region as a constraint condition to obtain second fitting information; and according to the first fitting information and the second fitting information, executing a molecular localization task of the fluorescence image to obtain molecular localization information of the fluorescence image. According to the invention, the positioning precision of the fluorescence image in the case of diffraction spot overlapping can be improved.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The invention relates to a method for detecting microRNA-21 (miR-21) based on DNA tetrahedrons with different functions and a spatial local cascade cycle catalytic amplification strategy. According to the method, two kinds of DNA tetrahedrons (a catalytic DNA tetrahedron Cat-DT and a signal DNA tetrahedron Sig-DT) with different functions are designed, and a cascade cycle amplification circuit is constructed. The miR-21 can trigger the release of an enzyme chain (E-DNA), the Cat-DT is converted into a three-branch DNA nanostructure (TB-DNA), each TB-DNA carries three E-DNA units, the conversion of more Cat-DT is further catalyzed, and the exponential amplification is realized. Meanwhile, TB-DNA and Sig-DT interact to generate a fluorescence signal, and a miR-21 simulation chain is regenerated to form a circulation mechanism, so that the reaction efficiency is remarkably improved. According to the method, the detection linear range is 600 fM to 100 nM, the detection limit is as low as 400 fM, and the total reaction time is shortened to 80 minutes. In addition, the method has good practical application capability and wide application prospect in the aspect of miR-21 detection.

The invention designs a preparation method of an electrochemical sensor for detecting acrylamide based on a CeZn-NC loaded PtPd nano composite material. CeZn-NC is obtained by calcining bimetallic CeZn-MOF with high electrochemical activity, and a large amount of PtPd nanoparticles are loaded by using the advantage of large surface area of CeZn-NC, so that the nanocomposite CeZn-NC-PtPd with high conductivity is obtained. According to the invention, acrylamide is taken as an aptamer, the aptamer is modified on an electrode through a physical adsorption effect, when acrylamide exists, acrylamide is combined with the aptamer, a large number of Y-type DNA nanostructures exist in a supernatant, and the Y-type structures are fixed on the electrode through platinum-sulfur bonds and palladium-sulfur bonds. Due to the Y-shaped structure, the signal probe MB / S1 / NiFe-Fe PBAatAu combined on the electrode is increased, and the signal is increased, so that the acrylamide is detected. The electrochemical biosensor constructed by the method disclosed by the invention has relatively high specificity and relatively high sensitivity.

The application belongs to the technical field of biosensing detection, and discloses a preparation method of a fluorescence aptamer sensor based on H-type DNA nanostructure and application thereof, wherein a certain amount of cDNA1, cDNA2 and a displacement strand ZH are mixed and incubated, then an aflatoxin B1 aptamer strand (AFB1-Apt) is added and incubated to obtain an H-type DNA nanostructure, and hollow carbon spheres are added to the above-mentioned mixed system and mixed and incubated; when AFB1 is absent, AFB1-Apt and ZH undergo strand displacement and are free in the system, at this time, ZH is adsorbed by a quenching material, so that the fluorescence thereof is quenched; when AFB1 exists, AFB1 preferentially combines with AFB1-Apt, at this time, ZH participating in the formation of the H-type structure is free in the system in a double-stranded structure, so that the fluorescence signal is maintained, and thus the preparation of the fluorescence aptamer sensor is completed; AFB1 is quantitatively analyzed according to the response signal change. According to the above-mentioned steps, a real sample with a standard addition is detected, and a recovery rate is calculated. The prepared fluorescence aptamer sensor has high sensitivity, good selectivity and excellent practical application ability.

The invention relates to a method for detecting the state of a DNA nanostructure based on co-localization of a single dye label and a subcellular organelle, and the detection method comprises the following steps: incubating a DNA nanostructure connected with a dye and cells, and detecting the co-localization coefficient of the dye and the cellular organelle; when the co-localization coefficient of the dye and the lysosome is greater than 0.5, judging that the DNA nanostructure is complete; when the co-localization coefficient of the dye and the mitochondria is greater than 0.5, judging that the DNA nano structure is damaged; and judging that the DNA nanostructure is partially complete under other conditions. According to the method for judging the fate of the DNA nanostructure and detecting the integrity of the DNA nanostructure based on co-localization of the single dye and the subcellular organelle, not only can the intact or damaged states of the same DNA nanostructure under different conditions be distinguished by utilizing the position of the DNA nanostructure in the subcellular organelle in the cell, but also the intracellular states of different DNA nanostructures can be detected.

The invention discloses a nanopore detection platform based on DNA nano transduction as well as a preparation method and application of the nanopore detection platform. The detection platform is used for realizing multi-disease diagnosis or tumor early screening through multi-marker recognition on the surface of the exosome. The device is composed of a programmable DNA nano transduction switch and a nanopore detection device. The DNA nano transduction switch is composed of a magnetic bead (Magnet Bead), a nucleic acid aptamer (Aptamer) and a tetrahedral DNA nano structure (TDN). The DNA nano transduction switch releases TDN when identifying one or more disease-related exosome protein markers at the same time, the TDN generates a standardized electrical event in the nanopore, and signal amplification and statistical readout are realized. By designing different nucleic acid aptamer combinations and machine learning classification, the method can be expanded into a diagnosis platform for various diseases, and is suitable for early screening, staging, curative effect monitoring and multi-disease concurrent detection.

Described herein is a DNA nanostructure-based platform that can modulate a number of cellular functions depending on the concentration and surface decoration of the nanostructure. Utilizing different peptides for surface functionalization of DNA nanostructures, lysosomal activity was able to be modulated which in turn translated into the control of cellular functionality, ranging from changes in cell morphology to modulation of immune signaling and cell death. This demonstrates the rational design of a new generation of versatile DNA-based nanoplatforms that can be used in various biomedical applications such as the development of combinatorial anti-cancer platforms, efficient systems for endolysosomal escape, and nanoplatforms modulating lysosomal pH.

The invention relates to the technical field of electrochemical biosensing, in particular to a sensing electrode for CRISPR-Cas electrochemical detection and a preparation method and application thereof. The invention relates to a sensing electrode for CRISPR-Cas electrochemical detection. The sensing electrode comprises a screen printing carbon electrode substrate, an NbC-MXene layer, an NG-PEI-COF layer and a coralline gold nanostructure layer, wherein the NbC-MXene layer, the NG-PEI-COF layer and the coralline-shaped gold nanostructure layer are fixedly modified on the surface of a working area of the screen printing carbon electrode substrate in sequence, and a tetrahedral DNA nanostructure probe is fixedly modified on the surface of the coralline-shaped gold nanostructure layer. The electrode has extremely high electrochemical activity, excellent electron conduction performance and stable biomolecule fixing capability, and can provide a sensitive and stable signal conversion platform for CRISPR-Cas detection.

The present invention relates to a composition for cryopreservation, comprising: a nucleic acid structure which comprises a scaffold nucleic acid folded at predetermined positions to form multiple strands, and a plurality of staple nucleic acids wherein at least a portion of a sequence thereof comprises a complementary sequence to that of the scaffold nucleic acid, which are bound to at least one of the strands of the scaffold nucleic acid to form a double strand; linkers coupled to at least one of single strands in the nucleic acid structure; and an anti-freezing peptide coupled to at least one of the linkers, so as to exhibit excellent freeze-protection effects, which in turn increase cell viability during cryopreservation of cells and tissues, while retaining original texture of food even when used for freezing the food.

The invention discloses a physical unclonable anti-counterfeiting material based on a DNA (Deoxyribose Nucleic Acid) nanostructure as well as a preparation method and application of the physical unclonable anti-counterfeiting material, and belongs to the technical field of information security and nano biology. According to the anti-counterfeiting material, three regular tetrahedron DNA monomers are connected through flexible double chains to form a DNA tripolymer, double-chain thermodynamic flexibility endows the anti-counterfeiting material with a nanoscale random geometric configuration, multi-dimensional encryption is achieved through different spectrum fluorophores modified randomly, and the information entropy reaches 4.3 bits / pixel. By combining deterministic self-assembly of the DNA nano-structure with dynamic flexibility, unification of deterministic preparation and random anti-counterfeiting characteristics is realized, and the method has excellent biocompatibility, high safety and strong expansibility, can be seamlessly compatible with a DNA computing system and a data storage system, and has wide application prospects. Multiple scenes such as information encryption authentication, biomedical authentication, flexible electronic anti-counterfeiting and DNA storage chip anti-counterfeiting are adapted, and an innovative platform is provided for the next generation of security authentication technology.

The invention relates to a DNA (deoxyribonucleic acid) nanostructure assembly and a method for detecting the integrity of a DNA nanostructure in a cell. The assembly comprises the DNA nanostructure and a dye connected with the DNA nanostructure, the dye comprises a cyanine fluorescent dye and a rhodamine fluorescent dye. According to the method, two dyes are connected to the DNA nanostructure, and the completeness of the DNA nanostructure in cells can be judged by utilizing the Pearson's correlation coefficient and judging the fluorescence signal correlation of the dyes. The method is high in judgment accuracy and simple in judgment method, the added double dyes are common fluorescent dyes, the influence on the drug effect is low, and the method can be used for intracellular integrity detection of various drugs containing the DNA nanostructure.

The invention relates to the technical field of electrochemical biosensors, in particular to an electrochemical biosensor for detecting domoic acid based on aptamer-regulated CRISPR (clustered regularly interspaced short palindromic repeats) activity as well as a preparation method and application of the electrochemical biosensor. The electrochemical biosensor comprises a'three-element 'detection system: 1, synthesizing a metal organic framework (UiO-66-NH2) loaded with methylene blue MB signal molecules as a signal label; 2, using rolling circle amplification (RCA) to construct a DNA nanostructure rich in repetitive sequences on the magnetic beads as a signal amplification platform; and 3, the sequence of the domoic acid aptamer is ingeniously designed into an activation switch of Cas12a. When target domoic acid exists, a Cas12a system is inactivated through competitive binding, so that a complete DNA nanostructure is reserved, a large number of signal tags are captured, and a strong electrochemical signal is generated.