44 results about "Dna nanostructure" patented technology

The invention relates to polymer nanoparticle and DNA nanostructure delivery compositions for non-viral delivery, and methods therefor. More particularly, the invention relates to polymer nanoparticle delivery compositions, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, and DNA nanostructure delivery compositions, such as DNA origami compositions, for the delivery of more than one payload, or for the delivery of a nucleic acid construct payload of 3 kB or more, and methods therefor.

The invention provides a DNA (Deoxyribose Nucleic Acid) nano-structure carrier. The DNA nano-structure carrier provided by the invention has an EGFR (epidermal growth factor receptor) aptamer structure and is used for loading a connecting sequence of siRNA; moreover, after the siRNA is loaded, the DNA nano-structure carrier can further load a chemotherapeutic drug such as adriamycin, so that a DNA nano-drug based on the DNA nano-structure carrier is obtained. Through the target gene expression knock-down effect of siME3, the cytotoxicity of adriamycin and the targeting property of the EGFR aptamer structure, the DNA nano-drug provided by the invention can effectively inhibit pancreatic cancer, especially the growth of ME2 deletion type pancreatic cancer cells.

The present invention discloses an information storage method based on DNA coding. The method comprises the following steps: converting digital information to be stored into a quaternary code, and converting the quaternary code into a DNA sequence consisting of adenine A, cytosine C, guanine G, and thymine T according to a preset mapping rule, and using the chemical modification state of the nucleotide pairs to represent an additional information dimension; inserting error detection and correction codes at every predetermined number of base positions in the DNA sequence, dividing the encoded DNA sequence into multiple fragments of 100-150 bp in length, and adding specific recognition sequences and index markers at both ends of each fragment; utilizing a reversibly thermoresponsive DNA nanostructure as a carrier, selectively binding the DNA fragments to the carrier, and realizing hierarchical storage and rapid retrieval of information through a temperature gradient control system; the present invention can significantly improve the information density and lifespan of DNA storage, and provides a new technical path for large-scale, long-term, and secure molecular information storage.

This work proposes a novel enzyme-free electrochemiluminescence sensing platform based on novel DNA-quantum dot nanostructures and hybridization chain reaction (HCR) amplification, and applies it to trace Cd. 2+ The detection; first, Cd 2+ Aptamer-triggered HCR amplification introduces a large amount of biotin-labeled DNA into the electrode, whereby biotin specifically captures a large amount of avidin SA-CdS quantum dot complexes, exhibiting a high ECL signal; target Cd 2+ Upon binding to its aptamer, the quantum dot-DNA structure detaches from the electrode, resulting in a significant decrease in the ECL signal, thus enabling the control of Cd. 2+ This sensor exhibits ultrasensitive detection with a dynamic response range from 10 fM to 10 nM and a detection limit of 2.6 fM. It demonstrates excellent selectivity, speed, high sensitivity, and practicality for detecting real water samples, providing a novel and competitive strategy for detecting heavy metal ions in real-world samples.

The invention discloses a molecular system based on DNA nano-structure artificial atoms and a construction and regulation method, and belongs to the technical field of DNA nanotechnology. The construction method of the molecular system comprises the following steps: mixing DNA chains with extension chains, adding a TM buffer solution, and carrying out annealing reaction to synthesize a DNA nano-structure with extension chains; dNA nanostructures are used for representing artificial atoms, extended chains on the DNA nanostructures are used for representing valence electrons, double chains formed by interaction among the extended chains are used for representing chemical bonds, a plurality of DNA nanostructures with extended chains are mixed in proportion according to a target molecular configuration, and a molecular system is obtained through one-pot assembly. Dynamic dissociation and recombination of artificial atoms are achieved based on DNA strand displacement reaction, and accurate regulation and control of a programmable molecular system are achieved. The construction precision and regulation effectiveness of the molecular system are verified through various technologies, the system has the advantages of being high in programmability, high in assembly efficiency, good in structural controllability and the like, and a brand new technical platform is provided for the fields of material design, chemical process simulation, biological engineering and the like.

The invention belongs to the technical field of biomedicine and nano crossing, and particularly relates to a biomineralization-based nucleic acid aptamer functionalized DNA nano-structure compound as well as a preparation method and application thereof. The compound has a tetrahedral structure and comprises a functional DNA tetrahedral framework and a calcium phosphate coating coated on the surface of the functional DNA tetrahedral framework, the functionalized DNA tetrahedral framework is formed by self-assembly of four DNA single chains; wherein the 5'end of one DNA single chain is modified with a nucleic acid aptamer. The bionic calcium coating is used as a protective shell, so that the in-vivo stability and the angiogenesis promoting activity of the Apt02 are remarkably improved. Meanwhile, by virtue of the specific spatial configuration of the tetrahedral DNA nanostructure, the compound can release calcium ions after being taken by cells, so that osteogenic differentiation is further promoted, and a new strategy for synergistic promotion is provided for vascularized bone regeneration.

Provided herein, in some embodiments, are nucleic acid nanostructure-based vaccines.

Described herein is a DNA nanostructure-based platform that can modulate a number of cellular functions depending on the concentration and surface decoration of the nanostructure. Utilizing different peptides for surface functionalization of DNA nanostructures, lysosomal activity was able to be modulated which in turn translated into the control of cellular functionality, ranging from changes in cell morphology to modulation of immune signaling and cell death. This demonstrates the rational design of a new generation of versatile DNA-based nanoplatforms that can be used in various biomedical applications such as the development of combinatorial anti-cancer platforms, efficient systems for endolysosomal escape, and nanoplatforms modulating lysosomal pH.

The invention relates to a double DNA nanosphere probe (DDNS) as well as a preparation method and biosensing application thereof. The DDNS comprises a DNA (deoxyribonucleic acid) nanosphere 1 (DNS-1) and a DNA nanosphere 2 (DNS-2). The DNS-1 and the DNS-2 are respectively formed by self-assembling two cholesterol modified DNA single-stranded probes with hairpin structures, and are respectively used for identifying target miRNA and triggering a non-enzymatic circulating strand displacement amplification (T-SDA) reaction. The DDNS can realize high-sensitivity fluorescence detection on target miRNA and can effectively inhibit non-target miRNA interference, and the interference inhibition rate is close to 100%. Besides, DDNS has excellent nuclease stability, the system can automatically enter living cells without a transfection reagent, high signal-to-noise ratio fluorescence differentiation of cancer cells and healthy cells is realized on the premise of maintaining cell activity, the performance is equivalent to that of a transfection agent dependent system, and the biological safety is remarkably improved. The invention provides a new material and a new method for application of the DNA nanostructure in the fields of molecular diagnosis and precision medical treatment.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

The invention provides a pellicle drug-loading system and a preparation method and application thereof, the pellicle drug-loading system is of a structure that an outer membrane wraps a core, and the outer membrane is a bacterial outer membrane; the core is formed by loading near-infrared two-region AIE photo-thermal molecules on a tetrahedral DNA (deoxyribonucleic acid) nano structure. The invention has the advantages that: the mycoderm drug delivery system can realize spatial dual targeting and time visual regulation and control on pathogens, and constructs a positive feedback cycle of drug delivery-immune activation through triple function synergy, thereby providing a drug basis for treatment of bacterial infection.

The invention discloses a fermentation medium for efficiently producing single-stranded DNA and a DNA nanostructure by a phagocytosis method and application of the fermentation medium, and relates to the technical field of biology. The fermentation culture medium is obtained by using ammonium chloride as a single inorganic nitrogen source to replace a nitrogen source (NH4) 2HPO4 in a Riesenberg culture medium. The invention also provides a method for efficiently producing single-stranded DNA (ssDNA) and a DNA nanostructure by a phagocytic grain method, high yield of the ssDNA can be promoted by adopting the fermentation culture medium, a nitrogen source is optimized and screened through the culture medium, and the invention finds that the yield of the single-stranded DNA can be increased by using ammonium chloride as a unique inorganic nitrogen source in the fermentation culture medium for production, and the yield of the single-stranded DNA can be increased by using the ammonium chloride as the unique inorganic nitrogen source in the fermentation culture medium. The yield of single-stranded DNA with triangular, hairpin rectangular, rod-like and quadrilateral DNA structures is increased to different extents by the fermentation culture medium, and a theoretical basis is provided for large-scale production of different types of ssDNA and DNA structures.

The application discloses a kind of space-time controllable T cell junction based on DNA nanostructure and its construction method and application, belong to DNA nanotechnology field.T cell junction includes six helix bundle paper structure and assembled internal functional layer and external shielding layer on it;Six helix bundle paper structure is assembled using 63 staple chain and M13;The internal functional layer includes nucleic acid-antibody conjugate combined on the four helix extension sequences of the top and bottom of the six helix bundle paper structure, and hand-in-hand palindromic sequence combined on the two helix extension sequences on left and right sides;External shielding layer includes C chain modified serum albumin binding peptide and chain I, and chain I includes pH response i-motif switch.T cell junction of the application has the space-time control ability of pH response and the potential of inducing TCR cluster to enhance T cell activation, has great potential to improve the safety and effectiveness of T cell immunotherapy.

The invention discloses a DNA (deoxyribonucleic acid) functionalized nanopore sensor based on nanobubbles, a preparation method of the DNA functionalized nanopore sensor and application of the DNA functionalized nanopore sensor in miRNA (micro Ribonucleic Acid) detection. The sensor comprises a conical nanopore, 3-aminopropyltriethoxysilane, a DNA nanostructure and hemin, wherein the conical nanopore is formed in a borosilicate glass tube, and the 3-aminopropyltriethoxysilane, the DNA nanostructure and the hemin are sequentially modified in the nanopore. The DNA nano structure is a hairpin structure, contains a target recognition sequence and a nucleic acid sequence rich in guanine, and can form a G-quadruplex / hemin compound with hemin after being combined with a target so as to generate oxygen nano bubbles. The sensor takes the nanobubbles as a signal output means, nanobubble pollution of an electrochemical reaction interface is effectively avoided, target detection is realized by performing electrochemical pulse counting on the nanobubbles, and the sensor has the advantages of low background noise, high efficiency, high sensitivity and the like. In addition, the nanopore sensor is low in cost, simple to operate and suitable for popularization and application.

The invention provides a DNA nano-structure connection method, system and product based on polymerase regulation and control, and belongs to the technical field of DNA paper-cut. The method comprises the following steps: respectively preparing a first DNA nano-structure and a second DNA nano-structure, a first connecting sequence in a first connecting chain on the first DNA nanostructure is complementary to a second connecting sequence in a second connecting chain on the second DNA nanostructure; mixing the first locked single strand with the first DNA nanostructure to form a first composite structure; mixing a second locked single strand with a second DNA nanostructure to form a second composite structure; placing the first composite structure and the second composite structure in the same reaction system; when connection of the first DNA nanostructure and the second DNA nanostructure needs to be achieved, primer chains and DNA polymerase are added into a reaction system, so that connection of the first DNA nanostructure and the second DNA nanostructure is completed through hybridization between complementary connecting chains.

The present invention relates to a composition for cryopreservation, comprising: a nucleic acid structure which comprises a scaffold nucleic acid folded at predetermined positions to form multiple strands, and a plurality of staple nucleic acids wherein at least a portion of a sequence thereof comprises a complementary sequence to that of the scaffold nucleic acid, which are bound to at least one of the strands of the scaffold nucleic acid to form a double strand; linkers coupled to at least one of single strands in the nucleic acid structure; and an anti-freezing peptide coupled to at least one of the linkers, so as to exhibit excellent freeze-protection effects, which in turn increase cell viability during cryopreservation of cells and tissues, while retaining original texture of food even when used for freezing the food.

The present invention provides a use of SQB-DNA nanostructures, prepared using DNA origami technology, for inducing differentiation into nerve cells or nerve cell regeneration, or treating nerve damage or neurological diseases. The present invention is the first instance of achieving nerve recovery through precise ligand presentation using DNA origami nanostructures, and the widespread use of SQB as a therapeutic drug for various types of nerve damage and neurological diseases requiring nerve regeneration is expected due to the provision of the present invention.

The application belongs to the field of biological computer technology and DNA nanotechnology, and particularly relates to a logic element based on a DNA nanostructure and application of the logic element in manufacturing a true random number generator. The logic element based on the DNA nanostructure takes a two-dimensional cross DNA nanostructure as a main body of the element, utilizes basic characteristics of the DNA nanostructure, such as uniform size, accurate site programming and rich extensible edge chains, constructs logic elements carrying different information through site programming, takes the extensible edge chains as sticky ends to realize a stable and reliable coaxial physical connection relationship between the logic elements, and only when there is a complementary relationship between the sticky ends of the logic elements, can a dimer or a multimer be formed through self-assembly. Therefore, when the logic element is applied to manufacturing the random number generator, the multimer connected by the cross paper folds still has good rigidity, and the correctness of the random generation result is ensured.

The application discloses a DNA nano antisense drug targeting microRNA, a preparation method and application thereof. The DNA nano antisense drug comprises a DNA nanostructure as a carrier, antisense peptide nucleic acid and selected aptamer, the DNA nanostructure wraps the antisense peptide nucleic acid, and exposes the selected aptamer, the antisense peptide nucleic acid is connected to the 3' end of a staple chain constituting the DNA nanostructure, and the selected aptamer is modified to the 5' end extended from another staple chain constituting the DNA nanostructure, and the antisense peptide nucleic acid can specifically recognize target microRNA in tumor cells. The DNA nano antisense drug has the advantages of simple preparation process, low price and harmless metabolic products, has strong specific binding effect on target microRNA and can promote cell apoptosis, and has great application value in the field of anticancer drug preparation.

The invention discloses a silicon dioxide cluster as well as a preparation method and application thereof. The method comprises the following steps: in an alkaline buffer solution, carrying out a dehydration condensation reaction by controlling the molar concentration of TMAPS and TEOS, and carrying out a curing process to obtain the monodisperse and super-stable cationic silicon dioxide cluster. The cationic silicon dioxide cluster prepared by the method disclosed by the invention not only can be used for accurate assembly and mineralization reaction of a DNA nano-structure template and provides a new way for construction of a functional silicon-based material, but also realizes effective stability of an intermediate process before and after nucleation; the general mechanism of template-oriented natural and bionic amorphous mineralization can be disclosed, and the method has important significance for promoting the development of bionic silicon-based materials.

The application relates to a graphene nanostructure preparation method, which comprises the following steps: providing a DNA nanostructure and a substrate with graphene; non-covalently modifying the graphene, and spreading the DNA nanostructure on the surface of the graphene; adding TMAPS and TEOS into a buffer solution to pre-hydrolyze and form a pre-hydrolysis solution, and silicifying the DNA nanostructure spread on the surface of the graphene in the pre-hydrolysis solution to grow a protective SiO2 shell on the surface of the DNA nanostructure, thereby obtaining a DNA-SiO2 mask; removing the graphene not covered by the DNA-SiO2 mask by using oxygen plasma etching; and removing the DNA-SiO2 mask to obtain the graphene nanostructure. According to the graphene nanostructure preparation method, the nano-etching technology based on the DNA-SiO2 mask solves the problem that the existing graphene nanostructure preparation technology cannot simultaneously meet the requirements of high precision, controllable morphology, low cost and large-scale preparation.

The invention discloses an electrocatalytic urea synthesis system and method based on a DNA nano-reactor, and belongs to the crossing field of electrochemical catalysis and nano-biotechnology. The system comprises a DNA nanoreactor, a catalytic activity center, an electrochemical device, an electric field regulation and control system and an in-situ monitoring and feedback system, the DNA nanoreactor adopts a DNA origami technology to construct a three-dimensional hollow structure and loads bimetallic nanoparticles, and efficient synthesis of urea is realized by accurately controlling reaction conditions. The local concentration of reactants is remarkably improved through the confinement effect and the molecular recognition function of the DNA nanostructure, the Faraday efficiency and the yield of urea are effectively improved, the reaction potential is reduced, and meanwhile accurate regulation and control of the reaction process are achieved through an intelligent monitoring system. The system can operate under the conditions of normal temperature and normal pressure, is suitable for resource utilization of industrial wastewater nitrate, has the characteristics of low energy consumption, environmental friendliness and the like, meets the requirements of green chemical development, and conforms to the national double-carbon policy.

The invention relates to the technical field of image processing, in particular to a fluorescence image signal enhancement system and method based on a three-dimensional DNA nanostructure. The method comprises the following steps: carrying out image identification on a fluorescence image to determine a plurality of diffraction spot areas; in the plurality of diffraction spot regions, distinguishing a first region corresponding to a single-molecule diffraction spot and a second region corresponding to an overlapped diffraction spot; fitting the gray level distribution of the first area to obtain first fitting information; distinguishing a mixed sub-region and a pure sub-region in the second region, and fitting the gray distribution of the pure sub-region by taking the gray distribution of the mixed sub-region as a constraint condition to obtain second fitting information; and according to the first fitting information and the second fitting information, executing a molecular localization task of the fluorescence image to obtain molecular localization information of the fluorescence image. According to the invention, the positioning precision of the fluorescence image in the case of diffraction spot overlapping can be improved.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The invention relates to active oxygen tolerance programmable DNA origami nanoparticles and a preparation method thereof.The preparation method comprises the following steps that in a buffer solution, an M13 support and a corresponding stapler chain are mixed according to the molar ratio of 1: 5, and then annealing is conducted; and after annealing, carrying out ultrafiltration treatment on the synthesized DNA origami nanoparticles to remove free stapler chains so as to obtain the DNA origami nanoparticles with programmable active oxygen tolerance. The invention further provides application of the active oxygen tolerance programmable DNA origami nanoparticles prepared on the basis of the method in preparation of detection sensors. Compared with the prior art, the relationship between the number of the cracks in the DNA origami and the tolerance to the active oxygen is found for the first time. Then, the found rule has important guiding significance for improving DNA origami and improving the active oxygen tolerance, and the biological application scene of the DNA nanostructure is greatly expanded.

The invention relates to a method for detecting microRNA-21 (miR-21) based on DNA tetrahedrons with different functions and a spatial local cascade cycle catalytic amplification strategy. According to the method, two kinds of DNA tetrahedrons (a catalytic DNA tetrahedron Cat-DT and a signal DNA tetrahedron Sig-DT) with different functions are designed, and a cascade cycle amplification circuit is constructed. The miR-21 can trigger the release of an enzyme chain (E-DNA), the Cat-DT is converted into a three-branch DNA nanostructure (TB-DNA), each TB-DNA carries three E-DNA units, the conversion of more Cat-DT is further catalyzed, and the exponential amplification is realized. Meanwhile, TB-DNA and Sig-DT interact to generate a fluorescence signal, and a miR-21 simulation chain is regenerated to form a circulation mechanism, so that the reaction efficiency is remarkably improved. According to the method, the detection linear range is 600 fM to 100 nM, the detection limit is as low as 400 fM, and the total reaction time is shortened to 80 minutes. In addition, the method has good practical application capability and wide application prospect in the aspect of miR-21 detection.

The invention belongs to the technical field of gene sequencing, and relates to a polymerase substrate for isolating fluorescent molecules by using a DNA (Deoxyribose Nucleic Acid) nanostructure as well as a preparation method and application of the polymerase substrate. According to the invention, the fluorescent molecules are specifically modified on one side of the DNA nanostructure, so that physical isolation between the polymerase and the fluorescent marker is realized, the design ingeniously utilizes the space spacing effect to protect the polymerase from potential interference of the fluorescent molecules, and accurate capture and interpretation of a fluorescent signal are guaranteed; therefore, the signal-to-noise ratio and the reliability of the whole biological detection system are improved.

The invention designs a preparation method of an electrochemical sensor for detecting acrylamide based on a CeZn-NC loaded PtPd nano composite material. CeZn-NC is obtained by calcining bimetallic CeZn-MOF with high electrochemical activity, and a large amount of PtPd nanoparticles are loaded by using the advantage of large surface area of CeZn-NC, so that the nanocomposite CeZn-NC-PtPd with high conductivity is obtained. According to the invention, acrylamide is taken as an aptamer, the aptamer is modified on an electrode through a physical adsorption effect, when acrylamide exists, acrylamide is combined with the aptamer, a large number of Y-type DNA nanostructures exist in a supernatant, and the Y-type structures are fixed on the electrode through platinum-sulfur bonds and palladium-sulfur bonds. Due to the Y-shaped structure, the signal probe MB / S1 / NiFe-Fe PBAatAu combined on the electrode is increased, and the signal is increased, so that the acrylamide is detected. The electrochemical biosensor constructed by the method disclosed by the invention has relatively high specificity and relatively high sensitivity.

The application belongs to the technical field of biosensing detection, and discloses a preparation method of a fluorescence aptamer sensor based on H-type DNA nanostructure and application thereof, wherein a certain amount of cDNA1, cDNA2 and a displacement strand ZH are mixed and incubated, then an aflatoxin B1 aptamer strand (AFB1-Apt) is added and incubated to obtain an H-type DNA nanostructure, and hollow carbon spheres are added to the above-mentioned mixed system and mixed and incubated; when AFB1 is absent, AFB1-Apt and ZH undergo strand displacement and are free in the system, at this time, ZH is adsorbed by a quenching material, so that the fluorescence thereof is quenched; when AFB1 exists, AFB1 preferentially combines with AFB1-Apt, at this time, ZH participating in the formation of the H-type structure is free in the system in a double-stranded structure, so that the fluorescence signal is maintained, and thus the preparation of the fluorescence aptamer sensor is completed; AFB1 is quantitatively analyzed according to the response signal change. According to the above-mentioned steps, a real sample with a standard addition is detected, and a recovery rate is calculated. The prepared fluorescence aptamer sensor has high sensitivity, good selectivity and excellent practical application ability.