Method for phase transition of hydrophobic nanoparticles by using DNA nanostructure

A technology of nanoparticles and nanostructures, applied in the field of DNA nanostructures

Inactive Publication Date: 2016-03-02
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, DNA nanostructure is used as a phase transformation reagent to carry out phase transformation of hydrophobic nanoparticles. In the literature and patents on DNA nanostructure at home and abroad, there is no report on using DNA nanostructure to carry out phase transformation of hydrophobic nanoparticles.

Method used

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  • Method for phase transition of hydrophobic nanoparticles by using DNA nanostructure
  • Method for phase transition of hydrophobic nanoparticles by using DNA nanostructure
  • Method for phase transition of hydrophobic nanoparticles by using DNA nanostructure

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Synthesis of functionalized DNA tetrahedra

[0021] Dilute DNA strands A, B, C and D in TM buffer (10mM Tris-HCl, 50mM MgCl 2 , pH 8.0) in a medium molar mix to a final concentration of 10 μM. After mixing the DNA, heat it to 95°C and then rapidly cool it to 4°C to obtain a DNA tetrahedral solution containing aptamers. Formation of DNA tetrahedral nanostructures can be verified by polyacrylamide gel electrophoresis (see figure 1 ).

[0022] Table 1 Detailed information on the DNA sequence used in the present invention

[0023]

Embodiment 2

[0025] Phase transition of iron oxide magnetic nanoparticles dispersed in chloroform

[0026] Slowly add 0.2mL chloroform solution containing 100μg / mL iron oxide nanoparticles (purchased from American Ocean Nanotechnology Company (Springdale, AR, USA)) to 0.2mL solution containing 10μM DNA tetrahedron. The iron nanoparticles are transferred from the chloroform layer to the water layer. Then move the aqueous solution into a microtube, centrifuge and wash with TM buffer, and redisperse in TM buffer to obtain water-soluble iron oxide nanoparticles.

[0027] attached figure 2 a, 2b are transmission electron microscope images of iron oxide nanoparticles before and after phase transition. attached figure 2 c is the dispersion diagram of iron oxide nanoparticles in chloroform and in water before and after phase transition, attached figure 2 d is a diagram of the magnetic separation phenomenon of iron oxide nanoparticles in aqueous solution after phase transition.

Embodiment 3

[0029] Phase transition of rare earth upconverting luminescent nanoparticles dispersed in cyclohexane

[0030] Slowly add 0.2 mL cyclohexane solution containing 100 μg / mL rare earth up-conversion luminescent nanoparticles to 0.2 mL aqueous solution containing 10 μM DNA tetrahedron. When the aqueous layer is reached, transfer the aqueous layer solution to a microtube. After the excess DNA tetrahedron is separated and washed by centrifugation, it is removed from the aqueous solution of the hydrophilic rare earth up-conversion luminescent nanoparticles. Finally, the hydrophilic rare earth up-conversion luminescent nanoparticles are redispersed in TM buffer solution to obtain the rare earth up-conversion luminescent nanoparticles dispersed in the aqueous solution.

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Abstract

Hydrophobic nanoparticles have great potential in biological analysis and biomedical application. The invention provides a simple method; a DNA nanostructure is used as a phase transition reagent; the DNA nanostructure is a DNA tetrahedral structure formed by self-assembly of four single-stranded DNAs, tail ends of three single-stranded DNAs of the four single-stranded DNAs are modified by carboxyl, the other one single-stranded DNA contains an aptamer, and the aptamer can be specifically combined with highly expressed nucleolin on a cancer cell membrane surface, so that the targeting ability of the nanoparticles is increased and the cell uptake ability is improved. The DNA tetrahedron as the phase transition reagent is non-toxic and convenient and only requires simple separation, and the high-stability and good-dispersion hydrophilic nanoparticles can be prepared.

Description

technical field [0001] The invention belongs to the fields of biological analysis and biomedical application, and in particular relates to synthesizing a DNA nanostructure containing an aptamer and using the DNA nanostructure for phase conversion of hydrophobic nanoparticle. Background technique [0002] Hydrophobic nanoparticles have special physical and chemical properties. With the development of nanoscience and technology, their potential application value in biomedicine has attracted the attention of researchers in various related fields, such as biosensing, cell imaging and cancer. treatment etc. The high magnetic susceptibility, chemical stability, and low toxicity of iron oxide nanoparticles have been intensively studied, and these properties make it useful not only as a magnetic resonance imaging T 2 Contrast agents are also widely used in drug delivery, cell tracking, and magnetic hyperthermia. [0003] At present, commercial nanoparticles are usually synthesize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/06
CPCC01G49/06C01P2004/04
Inventor 许小平吴淑贤杨黄浩李娟洪诚毅
Owner FUZHOU UNIV
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