Preparation and application method of dual bio-optical window targeting nano-bioprobe

A nano-biological probe and optical window technology, applied in the field of fluorescent bio-imaging, chemical/biological sensing, can solve the problem of rare dual bio-optical window materials, and achieve the effect of wide application prospects

A nano-biological probe and optical window technology, applied in the field of fluorescent bio-imaging, chemical/biological sensing, can solve the problem of rare dual bio-optical window materials, and achieve the effect of wide application prospects

CN107973901BActive Publication Date: 2020-02-14NANJING UNIV OF POSTS & TELECOMM
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  • Preparation and application method of dual bio-optical window targeting nano-bioprobe
  • Preparation and application method of dual bio-optical window targeting nano-bioprobe
  • Preparation and application method of dual bio-optical window targeting nano-bioprobe

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Embodiment 1

[0031] 1) Prepare monomer (1) by the following method, with (1) and (2) according to the material ratio of 1:1 feed intake, then in Pd(OAc) 2 / P(o-tolyl) 3 Under the catalysis, the two are coupled through the Heck reaction. After 12 hours of reaction, the neutral water-soluble conjugated polymer P1 is obtained; further quaternization reaction is carried out with methyl iodide, and the reaction is 3 days to obtain the cationic water-soluble conjugated polymer. Polymer P1'.

[0032]

[0033] 2) According to the charge ratio of 1:5, quickly inject the cationic water-soluble conjugated polymer P1'methanol solution prepared in step 1) into the HA aqueous solution under the ultrasonic environment, stir rapidly for 10 minutes, stir slowly for 6 hours, and statically Place overnight and freeze-dry to obtain targeted nano-biological probes. The structural formula of HA is as follows.

[0034]

[0035] 3) The targeted nano-biological probe prepared in step 2) is used for tumor-...

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Abstract

The invention discloses a preparation method and an application method of double biological optical window (650-1000nm) targeted nanometer biological probe. The preparation method comprises the following steps of performing organic metal catalyzed Heck reaction with a method of adopting bicomponent alternating copolymerization on a divinyl fluorene derivative and aromatic heterocyclic groups withnarrow band gaps to obtain a neutral polymer, and then performing quaternization to obtain a cationic water-soluble conjugated polymer with a main chain being of a D-pi-A structure and with two-photonabsorption and near infrared-emitting properties simultaneously, wherein an excitation wavelength and a emission wavelength of the cationic water-soluble conjugated polymer are in biological opticalwindows (i.e., double biological optical windows); and performing a nanoprecipitation method on the cationic water-soluble conjugated polymer and tumor-targeted biomolecules with opposite charges to prepare the targeted nanometer biological probe. By adopting the preparation method and the application method, double biological optical window targeted two-photon fluorocyte imaging can be realized.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing and fluorescent imaging materials, and specifically relates to the preparation of targeted nano-biological probes by combining cationic water-soluble conjugated polymers with two-photon absorption near-infrared emission and tumor-targeting biomolecules with opposite charges , and its applications in chemical / biological sensing and fluorescent bioimaging. Background technique [0002] Water-soluble conjugated polymers mainly refer to conjugated polymers with ionic functional group side chains introduced into the main chain. This type of polymer exhibits good stability in water or other polar organic solvents due to the introduction of functional group side chains. Solubility, while retaining the characteristics of conjugated polymers, such as: excellent semiconductor properties, high molar extinction coefficient, fluorescence signal amplification effect, etc. In recent years, w...

Claims

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Application Information

Patent Timeline
14 Feb 2020
Publication
CN107973901B
IPC
C08G61/12; G01N21/64
CPC
C08G61/123; C08G61/126; C08G2261/124; C08G2261/1424; C08G2261/143; C08G2261/18; C08G2261/3142; C08G2261/3223
Inventors
黄艳琴; 赵永康