The present invention relates to the technical field of life science and
medical imaging devices, and in particular, to a 3D
optical imaging system and method. The method comprises the following steps: S1, using a
CCD camera to acquire a two-dimensional bio-
optical image of an imaging target; S2, using the
CCD camera in step S1 in combination with
structured light to acquire a three-dimensional
surface contour image of the imaging target; S3, registering the two-dimensional bio-
optical image obtained in step S1 with the three-dimensional
surface contour image obtained in step S2; and S4, performing reconstruction to obtain a three-dimensional bio-
optical image. By means of using the same
CCD camera to acquire the two-dimensional bio-optical image and the three-dimensional
surface contour image of the imaging target, registering the two-dimensional bio-optical image with the three-dimensional surface contour image, using biological tissues with different optical properties to fill the three-dimensional surface contour image, and then combining the registered data, the three-dimensional bio-optical image can be obtained, thereby achieving the three-dimensional space distribution of bio-optical signals in the
organism.