9 results about "Cell hypoxia" patented technology

The application discloses a composition for lightening dark circles under eyes containing camellia chrysantha extract and a preparation method and application thereof, and relates to the technical field of cosmetics.The composition comprises component A, component B and component C;the component A comprises andrographis paniculata extract and camellia chrysantha extract;the component B comprises ubiquinone and safflower extract;and the component C is menthol lactate.The andrographis paniculata extract, the camellia chrysantha extract, the ubiquinone, the safflower extract and the menthol lactate are combined to have a synergistic effect; the composition formed by the specific combination of the components can promote blood microcirculation, inhibit microvascular generation, increase epidermal thickness, relieve cell hypoxia and eye fatigue, reduce blood vessel type dark circles, relieve redness and strengthen skin, and is mild and non-irritating to skin, and is suitable for people with sensitive skin.

The application discloses a Leuconostoc strain and application thereof, and the Leuconostoc strain HX-236 is screened by the inventors. The Leuconostoc strain HX-236 uses cordyceps militaris powder as the only nitrogen source, and after fermentation culture, a fermentation filtrate can be obtained. The fermentation filtrate has high contents of cordycepin and cordycepic acid, is easily soluble in water, is convenient to use, has good stability, has good compatibility, can significantly improve the expression of hypoxia inducible factor (HIF-1 alpha) in cells, thereby activating downstream signal pathways for regulating inflammatory reactions, cell autophagy and tissue neogenesis, and then regulating cell hypoxia pressure, improving problems of skin aging such as cell activity reduction, collagen loss, pigmentation and skin elasticity reduction caused by hypoxia, and having wide application prospects in the fields of medicines, cosmetics and the like.

The utility model relates to a low-oxygen cell culture bottle, which belongs to the technical field of low-oxygen cell culture and comprises a culture bottle, a circulation channel is arranged on a bottle body of the culture bottle, an oxygen removal cylinder is arranged on the circulation channel, an installation screen plate is fixedly installed on the inner side wall of the oxygen removal cylinder, and a circulation fan is fixedly installed on one side of the installation screen plate. According to the low-oxygen cell culture bottle, a circulating channel composed of a first hose, a deoxidizing cylinder, a second hose and a butt joint cover is arranged between a circulating outlet pipe and a circulating inlet pipe on the bottle body of the culture bottle, a deoxidizing material bag containing a deoxidant is arranged in the deoxidizing cylinder, and a circulating fan in the deoxidizing cylinder drives air in the culture bottle to flow; the low-oxygen cell culture device is simple in structure, convenient to operate and suitable for low-oxygen cell culture, and the problems that common low-oxygen cell culture equipment is high in cost and large in size, and waste is caused when large equipment is used during low-oxygen cell culture are solved.

Provided are a hyaluronic acid compound for resisting cell hypoxia injury, a preparation method therefor, and use thereof. The hyaluronic acid compound comprises hyaluronic acid with an average molecular weight in the ten-thousands range and hyaluronic acid with an average molecular weight in the hundred-thousands range according to a volume ratio of 1:3 to 3:1. Hyaluronic acid can inhibit hypoxia-induced overactivation of HIF-1α, reduce the reactive oxygen level in hypoxic cells, and reduce the level of inflammatory factors in the supernatant of hypoxic cells, thereby alleviating hypoxia-induced cell apoptosis. Therefore, hyaluronic acid can be used in the treatment of cell hypoxia injury. Compared with hyaluronic acid of a single molecular weight, the obtained hyaluronic acid compound has a synergistic effect.

This invention relates to the field of cell culture technology, and discloses a method for culturing NK cells and its applications. When NK cells enter a hypoxic environment, not only do their cytotoxic activity and proliferative capacity rapidly decline, but they also experience metabolic disorders such as impaired mitochondrial function, insufficient ATP production, and abnormal accumulation of reactive oxygen species (ROS), leading to functional failure and limiting their application in tumor therapy. Existing technologies mainly rely on constant or single-stage hypoxia induction, failing to improve the insufficient hypoxia adaptation of NK cells at the metabolic level. Therefore, this invention provides a method that uses repeated acclimatization culture with gradually decreasing oxygen concentration to allow NK cells to gradually adapt to the metabolic demands of a hypoxic environment. NK cells cultured using this method exhibit a more stable metabolic state and superior cytotoxic activity in a hypoxic environment, significantly enhancing their anti-tumor efficacy and solving the problem of metabolic imbalance and rapid functional loss of NK cells in the hypoxic tumor microenvironment in existing technologies.

The application provides a porous copper-manganese bimetallic nanomaterial applied to tumor immunotherapy and a preparation method thereof. The application obtains the hollow mesoporous copper-manganese bimetallic nanomaterial with uniform particle size through a cuprous oxide self-template etching method, then the nanomaterial is used to load a photosensitizer IR820 due to excellent drug loading capacity, and finally the nanomaterial is modified by mercapto polyethylene glycol to increase the dispersibility of the nanoparticles. The preparation method is stable and reliable, low in cost and wide in source, and has the advantages of simple process, practicality, strong controllability and the like. The nanomaterial has multifunctionality, can realize synergistic treatment of photothermal treatment / photodynamic treatment / chemical dynamic treatment, improve cell hypoxia, consume glutathione, remodel a tumor microenvironment, finally induce immunogenic death of tumor cells, enhance the immune response of tumor cells, efficiently inhibit tumor growth and metastasis, and has a broad application prospect.

The invention discloses a method for improving cell hypoxia tolerance by knocking out a PAXIP1 gene, belongs to the field of gene engineering, and proves that the PAXIP1 gene knockout can remarkably enhance the hypoxia toxicity resistance of cells to CoCl2 induction for the first time by constructing a pig small intestine epithelial cell model with the PAXIP1 gene knockout. The cell death rate of a gene knockout group is remarkably reduced, and the cell survival rate is remarkably improved. The result shows that PAXIP1 gene knockout can activate an anti-hypoxia protection mechanism of porcine small intestine epithelial cells, and reveals direct association between the PAXIP1 gene and animal hypoxia adaptability. The invention provides a new key functional gene target for high-altitude hypoxia adaptability research, and has potential application value in the fields of animal breeding and hypoxia-related disease treatment.

The invention belongs to the technical field of medicine application and development, and particularly relates to application of a musk heart-dredging dropping pill. The musk heart-dredging dropping pill is used for treating and / or relieving cardiovascular diseases, and the cardiovascular diseases comprise inflammation and / or cell hypoxia injury caused by cardiovascular lesion and / or injury and / or chronic heart failure. The musk heart-smoothing dropping pill can also be used for preparing medicines and / or health-care foods for improving inflammation and / or cell hypoxia injury caused by cardiovascular diseases and / or injury and / or chronic heart failure. The invention provides a new application field and direction of the musk heart-dredging dropping pill, develops the application of the musk heart-dredging dropping pill, and enables the musk heart-dredging dropping pill to have the value of further processing for indirect application.

The invention relates to the field of image-assisted analysis, in particular to a myocardial AC16 cell hypoxia damage inhibition method, which comprises the following steps: acquiring image data of a plurality of myocardial AC16 cells in a culture dish to extract spatial distribution characteristics of the myocardial AC16 cells in a culture dish area; determining a hypoxia damage characterization value of the myocardial AC16 cells according to the spatial distribution characteristics in combination with the corresponding deformation fitting values, so as to divide damage degree categories of the myocardial AC16 cells; according to the method, the myocardial AC16 cells in the culture dish are adaptively adjusted and inhibited on the basis of the injury degree category, the inhibiting effect of the transforming growth factor on the myocardial AC16 cells with low-oxygen injury is analyzed through image assistance, and the effectiveness of experiments is guaranteed.