3 results about "Cell hypoxia" patented technology

The application discloses a composition for lightening dark circles under eyes containing camellia chrysantha extract and a preparation method and application thereof, and relates to the technical field of cosmetics.The composition comprises component A, component B and component C;the component A comprises andrographis paniculata extract and camellia chrysantha extract;the component B comprises ubiquinone and safflower extract;and the component C is menthol lactate.The andrographis paniculata extract, the camellia chrysantha extract, the ubiquinone, the safflower extract and the menthol lactate are combined to have a synergistic effect; the composition formed by the specific combination of the components can promote blood microcirculation, inhibit microvascular generation, increase epidermal thickness, relieve cell hypoxia and eye fatigue, reduce blood vessel type dark circles, relieve redness and strengthen skin, and is mild and non-irritating to skin, and is suitable for people with sensitive skin.

This invention relates to the field of cell culture technology, and discloses a method for culturing NK cells and its applications. When NK cells enter a hypoxic environment, not only do their cytotoxic activity and proliferative capacity rapidly decline, but they also experience metabolic disorders such as impaired mitochondrial function, insufficient ATP production, and abnormal accumulation of reactive oxygen species (ROS), leading to functional failure and limiting their application in tumor therapy. Existing technologies mainly rely on constant or single-stage hypoxia induction, failing to improve the insufficient hypoxia adaptation of NK cells at the metabolic level. Therefore, this invention provides a method that uses repeated acclimatization culture with gradually decreasing oxygen concentration to allow NK cells to gradually adapt to the metabolic demands of a hypoxic environment. NK cells cultured using this method exhibit a more stable metabolic state and superior cytotoxic activity in a hypoxic environment, significantly enhancing their anti-tumor efficacy and solving the problem of metabolic imbalance and rapid functional loss of NK cells in the hypoxic tumor microenvironment in existing technologies.

The application provides a porous copper-manganese bimetallic nanomaterial applied to tumor immunotherapy and a preparation method thereof. The application obtains the hollow mesoporous copper-manganese bimetallic nanomaterial with uniform particle size through a cuprous oxide self-template etching method, then the nanomaterial is used to load a photosensitizer IR820 due to excellent drug loading capacity, and finally the nanomaterial is modified by mercapto polyethylene glycol to increase the dispersibility of the nanoparticles. The preparation method is stable and reliable, low in cost and wide in source, and has the advantages of simple process, practicality, strong controllability and the like. The nanomaterial has multifunctionality, can realize synergistic treatment of photothermal treatment / photodynamic treatment / chemical dynamic treatment, improve cell hypoxia, consume glutathione, remodel a tumor microenvironment, finally induce immunogenic death of tumor cells, enhance the immune response of tumor cells, efficiently inhibit tumor growth and metastasis, and has a broad application prospect.