18 results about "Cell hypoxia" patented technology

The invention discloses a dark circle fading composition containing a golden camellia extract as well as a preparation method and application of the dark circle fading composition, and relates to the technical field of cosmetics. The composition comprises a component A, a component B and a component C, the component A comprises a common andrographis herb extract and a golden camellia extract; the component B comprises ubiquinone and a saffron crocus extract; and the component C is menthol lactate. The herba andrographitis extract, the camellia nitidissima extract, the ubiquinone, the saffron crocus extract and the menthol lactate are combined and found to have a synergistic interaction effect, and the composition formed by the components through specific compatibility can promote blood microcirculation, inhibit microangiogenesis, increase epidermal thickness and relieve cell hypoxia and eye fatigue; moreover, the eye cream is mild and non-irritating to the skin, and is suitable for people with sensitive skin to use.

The present invention provides the use of Yangxue Oral Liquid in the preparation of drugs for neuroprotection against Alzheimer's disease and / or Parkinson's disease, and belongs to the field of biopharmaceutical technology. The present invention provides the use of Yangxue Oral Liquid in the preparation of drugs for neuroprotection against Alzheimer's disease and / or Parkinson's disease. By verifying the effects of Yangxue Oral Liquid on the amyloid beta-induced neuronal apoptosis model, the cobalt chloride-induced SH‑SY5Y cell hypoxia model, and the LPS-induced SH‑SY5Y cell inflammation model, the results showed that Yangxue Oral Liquid has a neuroprotective effect against Alzheimer's disease and / or Parkinson's disease. Therefore, the application provided by the present invention provides a new means for neuroprotection against Alzheimer's disease and / or Parkinson's disease.

The application discloses a composition for lightening dark circles under eyes containing camellia chrysantha extract and a preparation method and application thereof, and relates to the technical field of cosmetics.The composition comprises component A, component B and component C;the component A comprises andrographis paniculata extract and camellia chrysantha extract;the component B comprises ubiquinone and safflower extract;and the component C is menthol lactate.The andrographis paniculata extract, the camellia chrysantha extract, the ubiquinone, the safflower extract and the menthol lactate are combined to have a synergistic effect; the composition formed by the specific combination of the components can promote blood microcirculation, inhibit microvascular generation, increase epidermal thickness, relieve cell hypoxia and eye fatigue, reduce blood vessel type dark circles, relieve redness and strengthen skin, and is mild and non-irritating to skin, and is suitable for people with sensitive skin.

The application discloses a Leuconostoc strain and application thereof, and the Leuconostoc strain HX-236 is screened by the inventors. The Leuconostoc strain HX-236 uses cordyceps militaris powder as the only nitrogen source, and after fermentation culture, a fermentation filtrate can be obtained. The fermentation filtrate has high contents of cordycepin and cordycepic acid, is easily soluble in water, is convenient to use, has good stability, has good compatibility, can significantly improve the expression of hypoxia inducible factor (HIF-1 alpha) in cells, thereby activating downstream signal pathways for regulating inflammatory reactions, cell autophagy and tissue neogenesis, and then regulating cell hypoxia pressure, improving problems of skin aging such as cell activity reduction, collagen loss, pigmentation and skin elasticity reduction caused by hypoxia, and having wide application prospects in the fields of medicines, cosmetics and the like.

The invention discloses a method for improving cell hypoxia tolerance by knocking out an SLC5A3 gene, belongs to the field of gene engineering, and finds and proves that after the SLC5A3 gene is knocked out, hypoxia-induced cell death can be reduced and cell survival can be promoted for the first time by constructing an SLC5A3 gene knocked out pig small intestine epithelial cell model. It is revealed that the SLC5A3 gene is directly associated with the animal hypoxia adaptability. The invention provides a new key functional gene target for high-altitude hypoxia adaptability research, and has potential application value in the fields of animal breeding and hypoxia-related disease treatment.

The utility model relates to a low-oxygen cell culture bottle, which belongs to the technical field of low-oxygen cell culture and comprises a culture bottle, a circulation channel is arranged on a bottle body of the culture bottle, an oxygen removal cylinder is arranged on the circulation channel, an installation screen plate is fixedly installed on the inner side wall of the oxygen removal cylinder, and a circulation fan is fixedly installed on one side of the installation screen plate. According to the low-oxygen cell culture bottle, a circulating channel composed of a first hose, a deoxidizing cylinder, a second hose and a butt joint cover is arranged between a circulating outlet pipe and a circulating inlet pipe on the bottle body of the culture bottle, a deoxidizing material bag containing a deoxidant is arranged in the deoxidizing cylinder, and a circulating fan in the deoxidizing cylinder drives air in the culture bottle to flow; the low-oxygen cell culture device is simple in structure, convenient to operate and suitable for low-oxygen cell culture, and the problems that common low-oxygen cell culture equipment is high in cost and large in size, and waste is caused when large equipment is used during low-oxygen cell culture are solved.

Provided are a hyaluronic acid compound for resisting cell hypoxia injury, a preparation method therefor, and use thereof. The hyaluronic acid compound comprises hyaluronic acid with an average molecular weight in the ten-thousands range and hyaluronic acid with an average molecular weight in the hundred-thousands range according to a volume ratio of 1:3 to 3:1. Hyaluronic acid can inhibit hypoxia-induced overactivation of HIF-1α, reduce the reactive oxygen level in hypoxic cells, and reduce the level of inflammatory factors in the supernatant of hypoxic cells, thereby alleviating hypoxia-induced cell apoptosis. Therefore, hyaluronic acid can be used in the treatment of cell hypoxia injury. Compared with hyaluronic acid of a single molecular weight, the obtained hyaluronic acid compound has a synergistic effect.

This invention relates to the field of cell culture technology, and discloses a method for culturing NK cells and its applications. When NK cells enter a hypoxic environment, not only do their cytotoxic activity and proliferative capacity rapidly decline, but they also experience metabolic disorders such as impaired mitochondrial function, insufficient ATP production, and abnormal accumulation of reactive oxygen species (ROS), leading to functional failure and limiting their application in tumor therapy. Existing technologies mainly rely on constant or single-stage hypoxia induction, failing to improve the insufficient hypoxia adaptation of NK cells at the metabolic level. Therefore, this invention provides a method that uses repeated acclimatization culture with gradually decreasing oxygen concentration to allow NK cells to gradually adapt to the metabolic demands of a hypoxic environment. NK cells cultured using this method exhibit a more stable metabolic state and superior cytotoxic activity in a hypoxic environment, significantly enhancing their anti-tumor efficacy and solving the problem of metabolic imbalance and rapid functional loss of NK cells in the hypoxic tumor microenvironment in existing technologies.

The invention relates to the technical field of cell hypoxia culture, in particular to a device for manufacturing a chronic hypoxia environment for cell culture, which comprises a cell culture plate and a plate cover, a circle of connecting piece with an L-shaped section is arranged on the periphery of the bottom of the cell culture plate, and the horizontal end part of the connecting piece is fixed at the bottom of the outer surface of the cell culture plate; an outer cover corresponding to the connecting piece is arranged above the plate cover, the lower end face of the outer cover is downwards connected to the upper end face of the connecting piece, an oxygen consumption layer is formed among the outer cover, the connecting piece, the cell culture plate and the plate cover, and an oxygen consumption agent is arranged in the oxygen consumption layer; the connecting piece and the outer cover are arranged outside the cell culture plate to form the oxygen consumption layer, the outer cover or the plate cover is fixed through the lifting fixing mechanism, the cell culture plate and the plate cover are wrapped with the oxygen permeation membrane, the outer cover and the connecting piece are sealed through the sealing membrane, and the oxygen consumption agent is arranged in the oxygen consumption layer. The oxygen in the oxygen consumption layer and the cell culture plate is gradually consumed, a chronic oxygen-deficient environment is formed, the structure is simple, the cost is low, and the sealing performance is good.

The invention particularly relates to application of a traditional Chinese medicine monomer in treatment of vascular dementia. The component of the traditional Chinese medicine monomer is astragaloside III. Animal experiments prove that the astragaloside III can improve the problem of poor spatial learning ability and memory ability of a mouse with vascular dementia, and also has a treatment effect on a brain function lesion region caused by vascular dementia. A cell hypoxia and sugar deficiency damage model is utilized to verify that the protection effect of the astragaloside III is better than that of astragaloside I and astragaloside IV, and it is verified that the astragaloside III has a synergistic effect in common administration with a positive drug donepezil.

A method, device, electronic device and storage medium for screening anti-hypoxic drugs provided by the present invention relate to the technical field of drug screening. The method obtains the whole-field images of each cell group cultured in a non-hypoxic environment, cultured in a hypoxic environment without drug treatment, and cultured in a hypoxic environment with a candidate drug treatment. Using a pre-trained cell portrait segmentation network, subcellular structure images of multiple cells in each cell group are extracted from the whole-field images of each cell group and input into a pre-trained cell hypoxia scoring network to obtain the hypoxia scores of multiple cells in each cell group. According to the hypoxia scores of multiple cells in each cell group, it is determined whether the candidate drug is a drug that can improve the cell survival state under hypoxic conditions, thereby improving the screening speed and the accuracy of the screening results.

The application provides a porous copper-manganese bimetallic nanomaterial applied to tumor immunotherapy and a preparation method thereof. The application obtains the hollow mesoporous copper-manganese bimetallic nanomaterial with uniform particle size through a cuprous oxide self-template etching method, then the nanomaterial is used to load a photosensitizer IR820 due to excellent drug loading capacity, and finally the nanomaterial is modified by mercapto polyethylene glycol to increase the dispersibility of the nanoparticles. The preparation method is stable and reliable, low in cost and wide in source, and has the advantages of simple process, practicality, strong controllability and the like. The nanomaterial has multifunctionality, can realize synergistic treatment of photothermal treatment / photodynamic treatment / chemical dynamic treatment, improve cell hypoxia, consume glutathione, remodel a tumor microenvironment, finally induce immunogenic death of tumor cells, enhance the immune response of tumor cells, efficiently inhibit tumor growth and metastasis, and has a broad application prospect.

The invention provides a bovine primary cardiac fibroblast hypoxia model construction method, and belongs to the technical field of cell model construction, trypsin and type II collagenase are adopted for sequentially digesting, differential attachment and the like to obtain bovine primary cardiac fibroblasts, the bovine primary cardiac fibroblasts are treated for 24-72 h under the hypoxia condition of 1% O2, 5% CO2 and 94% N2 at the inoculation cell density of 3.5 * 10 < 5 >-8 * 10 < 5 > / ml, and the bovine primary cardiac fibroblasts are obtained. And constructing the cell hypoxia model. The three-gas incubator hypoxia model based on the primary bovine myocardial fibroblasts can accurately simulate a pathological hypoxia environment, and is suitable for hypoxia stress mechanism research and key molecule screening. The model is high in physiological correlation and simple and convenient to operate, has wide applicability and reference value, and provides a reliable experimental platform for research on the bovine hypoxia myocardial diseases.

The invention discloses a method for improving cell hypoxia tolerance by knocking out a PAXIP1 gene, belongs to the field of gene engineering, and proves that the PAXIP1 gene knockout can remarkably enhance the hypoxia toxicity resistance of cells to CoCl2 induction for the first time by constructing a pig small intestine epithelial cell model with the PAXIP1 gene knockout. The cell death rate of a gene knockout group is remarkably reduced, and the cell survival rate is remarkably improved. The result shows that PAXIP1 gene knockout can activate an anti-hypoxia protection mechanism of porcine small intestine epithelial cells, and reveals direct association between the PAXIP1 gene and animal hypoxia adaptability. The invention provides a new key functional gene target for high-altitude hypoxia adaptability research, and has potential application value in the fields of animal breeding and hypoxia-related disease treatment.

The invention belongs to the technical field of medicine application and development, and particularly relates to application of a musk heart-dredging dropping pill. The musk heart-dredging dropping pill is used for treating and / or relieving cardiovascular diseases, and the cardiovascular diseases comprise inflammation and / or cell hypoxia injury caused by cardiovascular lesion and / or injury and / or chronic heart failure. The musk heart-smoothing dropping pill can also be used for preparing medicines and / or health-care foods for improving inflammation and / or cell hypoxia injury caused by cardiovascular diseases and / or injury and / or chronic heart failure. The invention provides a new application field and direction of the musk heart-dredging dropping pill, develops the application of the musk heart-dredging dropping pill, and enables the musk heart-dredging dropping pill to have the value of further processing for indirect application.

The invention belongs to the field of anti-tumor materials, and provides drug-loaded hydrogel for tumor immunotherapy as well as a preparation method and application of the drug-loaded hydrogel. The drug-loaded injectable hydrogel is composed of matrix hydrogel, polypolyphenol nanoparticles and an immune checkpoint inhibitor, the polypolyphenol nanoparticles and the immune checkpoint inhibitor are distributed in the matrix hydrogel, the polypolyphenol nanoparticles are nanoparticles formed by self-assembly of polyethylene glycol, one end of the polyethylene glycol is connected with epigallocatechin gallate, and the other end of the polyethylene glycol is connected with the immune checkpoint inhibitor. The polypolyphenol nanoparticles inhibit expression of tumor cell hypoxia-inducible factors by inhibiting expression of tumor cell hypoxia-inducible factors and further inhibit expression of tumor cell PD-L1 antibodies, so that the sensitivity degree of tumor cells to immune checkpoint inhibitors is improved. The tumor inhibition effect is enhanced through the synergistic effect of the polypolyphenol nanoparticles and the immune checkpoint inhibitor, and meanwhile, the toxicity of the polypolyphenol nanoparticles to normal cells is relatively low; the problems that in the prior art, chemotherapy drugs and immunotherapy drugs are combined for use, the toxic and side effects of the chemotherapy drugs are large, and drug resistance is generated can be solved.

The utility model discloses a cell exposure hypoxia environment experiment device, which relates to the technical field of cell hypoxia experiments and comprises a support base and a ventilation component, an experiment box is mounted at the front end of the upper surface of the support base, and the ventilation component is arranged on the rear surface of the experiment box. The ventilation assembly comprises a dustproof ventilation opening, a ventilator, a protection frame, a mounting part, a clamping hole, a clamping part, a sealing plate and a rubber sealing gasket, the dustproof ventilation opening is formed in the lower end of the rear surface of the experiment box, the ventilator is mounted on the rear side of the dustproof ventilation opening, and the protection frame is arranged on the outer side of the ventilator. According to the cell exposure hypoxia environment experiment device, rapid ventilation can be achieved through the ventilation assembly, the recovery time of internal oxygen concentration and carbon dioxide concentration is shortened, concentration balance is ensured, multiple groups of cell experiments can be conducted at the same time through the limiting assembly according to needs, and the experiment efficiency is improved. And the cell culture dish can be limited, so that the cell culture dish is prevented from shaking or falling off during taking and placing.

The invention relates to the field of image-assisted analysis, in particular to a myocardial AC16 cell hypoxia damage inhibition method, which comprises the following steps: acquiring image data of a plurality of myocardial AC16 cells in a culture dish to extract spatial distribution characteristics of the myocardial AC16 cells in a culture dish area; determining a hypoxia damage characterization value of the myocardial AC16 cells according to the spatial distribution characteristics in combination with the corresponding deformation fitting values, so as to divide damage degree categories of the myocardial AC16 cells; according to the method, the myocardial AC16 cells in the culture dish are adaptively adjusted and inhibited on the basis of the injury degree category, the inhibiting effect of the transforming growth factor on the myocardial AC16 cells with low-oxygen injury is analyzed through image assistance, and the effectiveness of experiments is guaranteed.