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Preparation method and application method of double biological optical window targeted nanometer biological probe

A nano-biological probe and optical window technology, which is applied in the application fields of chemical/biological sensing and fluorescence bioimaging, can solve the problems of rare dual biological optical window materials, and achieve the effect of wide application prospects.

Active Publication Date: 2018-05-01
NANJING UNIV OF POSTS & TELECOMM
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Many water-soluble conjugated polymers studied in the past have only excitation or emission wavelengths in the bio-optical window, and dual bio-optical window materials with both excitation and emission wavelengths in the bio-optical window are still very rare

Method used

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  • Preparation method and application method of double biological optical window targeted nanometer biological probe
  • Preparation method and application method of double biological optical window targeted nanometer biological probe
  • Preparation method and application method of double biological optical window targeted nanometer biological probe

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Embodiment 1

[0031] 1) Prepare monomer (1) by the following method, with (1) and (2) according to the material ratio of 1:1 feed intake, then in Pd(OAc) 2 / P(o-tolyl) 3 Under the catalysis, the two are coupled through the Heck reaction. After 12 hours of reaction, the neutral water-soluble conjugated polymer P1 is obtained; further quaternization reaction is carried out with methyl iodide, and the reaction is 3 days to obtain the cationic water-soluble conjugated polymer. Polymer P1'.

[0032]

[0033] 2) According to the charge ratio of 1:5, quickly inject the cationic water-soluble conjugated polymer P1'methanol solution prepared in step 1) into the HA aqueous solution under the ultrasonic environment, stir rapidly for 10 minutes, stir slowly for 6 hours, and statically Place overnight and freeze-dry to obtain targeted nano-biological probes. The structural formula of HA is as follows.

[0034]

[0035] 3) The targeted nano-biological probe prepared in step 2) is used for tumor-...

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Abstract

The invention discloses a preparation method and an application method of double biological optical window (650-1000nm) targeted nanometer biological probe. The preparation method comprises the following steps of performing organic metal catalyzed Heck reaction with a method of adopting bicomponent alternating copolymerization on a divinyl fluorene derivative and aromatic heterocyclic groups withnarrow band gaps to obtain a neutral polymer, and then performing quaternization to obtain a cationic water-soluble conjugated polymer with a main chain being of a D-pi-A structure and with two-photonabsorption and near infrared-emitting properties simultaneously, wherein an excitation wavelength and a emission wavelength of the cationic water-soluble conjugated polymer are in biological opticalwindows (i.e., double biological optical windows); and performing a nanoprecipitation method on the cationic water-soluble conjugated polymer and tumor-targeted biomolecules with opposite charges to prepare the targeted nanometer biological probe. By adopting the preparation method and the application method, double biological optical window targeted two-photon fluorocyte imaging can be realized.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing and fluorescent imaging materials, and specifically relates to the preparation of targeted nano-biological probes by combining cationic water-soluble conjugated polymers with two-photon absorption near-infrared emission and tumor-targeting biomolecules with opposite charges , and its applications in chemical / biological sensing and fluorescent bioimaging. Background technique [0002] Water-soluble conjugated polymers mainly refer to conjugated polymers with ionic functional group side chains introduced into the main chain. This type of polymer exhibits good stability in water or other polar organic solvents due to the introduction of functional group side chains. Solubility, while retaining the characteristics of conjugated polymers, such as: excellent semiconductor properties, high molar extinction coefficient, fluorescence signal amplification effect, etc. In recent years, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G61/12G01N21/64
CPCC08G61/123C08G61/126C08G2261/124C08G2261/1424C08G2261/143C08G2261/18C08G2261/3142C08G2261/3223C08G2261/3246C08G2261/413C08G2261/522C08G2261/94G01N21/6402G01N21/6458G01N21/6486
Inventor 黄艳琴赵永康陈昊江荣翠刘兴奋范曲立黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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