AsPNA (antisense peptide nucleic acid)-TDN (tetrahedral DNA nanostructure) carrier compound as well as preparation method and application thereof

A technology of peptide nucleic acid and tetrahedron, which is applied in the field of antisense peptide nucleic acid-DNA tetrahedron carrier complex and its preparation, can solve the problems of strong cytotoxicity and limited use, and achieve good application prospects

Inactive Publication Date: 2018-09-07
SICHUAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned problems in the prior art, the present invention provides an antisense peptide nucleic acid-DNA tetrahedron carrier complex and its preparation method and application, which can effectively solve the problem of strong cytotoxicity and Use limited questions

Method used

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  • AsPNA (antisense peptide nucleic acid)-TDN (tetrahedral DNA nanostructure) carrier compound as well as preparation method and application thereof
  • AsPNA (antisense peptide nucleic acid)-TDN (tetrahedral DNA nanostructure) carrier compound as well as preparation method and application thereof
  • AsPNA (antisense peptide nucleic acid)-TDN (tetrahedral DNA nanostructure) carrier compound as well as preparation method and application thereof

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Embodiment 1

[0026] The construction of the tetrahedron carrier system (P-TDNs) of embodiment 1 carrying PNA

[0027] 1. Construction process

[0028] Each ssDNA single strand (S1, S2, S3, S4) and ftsz-asPNA single strand were added with TM buffer (10mM Tris-HCl, 50mM MgCl 2 ,pH 8.0) EP tube, vortexed to mix, centrifuged, and then placed in a PCR instrument, the temperature was rapidly raised to 95°C for 10min, and then rapidly cooled to 4°C for 20min, to synthesize P-TDNs with a concentration of about is 1000nM, and its construction process is as follows figure 1 shown.

[0029] Among them, the specific sequences of the four DNA single strands are as follows:

[0030] S1:

[0031] 5'-ATTTATTCACCCGCCATAGTAGACGTATCACCAGGCAGTTGAGACGAACATTCCTAAGTCTGAA-3' (SEQ ID NO: 1);

[0032] S2:

[0033] 5'-CACGATTCAGACTTAGGAATGTTCGACATGCGAGGGTCCAATACCGACGAT-3' (SEQ ID NO: 2);

[0034] S3:

[0035] 5'-ACTACTATGGCGGGTGATAAAACGTGACTATGTTTGAAATCGACGGGAAGAGCATGCCCATCC-3' (SEQ ID NO: 3);

[0036] S4: ...

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Abstract

The invention provides an asPNA (antisense peptide nucleic acid)-TDN (tetrahedral DNA nanostructure) carrier compound as well as a preparation method and an application thereof. The carrier compound contains asPNA capable of inhibiting MRSA (Methicillin-resistant Staphylococcus aureus) growth and a TDN, and a molar ratio of asPNA to the TDN is 1:1. The TDN and asPNA are bonded, one novel transmembrane carrier is formed through bonding of the TDN and asPNA on the basis that original excellent properties of the TDN and asPNA are reserved, and the problem that cellular uptake rate is affected byunmodified asPNA during application of asPNA at present is solved; the compound has a new cell target position, has an antibacterial mechanism which is completely different from that of a traditionalantibacterial drug, can be successfully taken by highly drug-resistant bacteria MRSA and can effectively inhibit the MRSA growth; besides, the prepared compound is free of cell specificity and toxicity.

Description

technical field [0001] The invention belongs to the technical field of drug-carrying systems, and in particular relates to an antisense peptide nucleic acid-DNA tetrahedron carrier complex and its preparation method and application. Background technique [0002] DNA tetrahedron (TDNs) is a nanomaterial with excellent performance, non-toxic, good biocompatibility and biodegradability, excellent cell membrane permeability, especially precise structure controllability, which makes it possess Great application potential. At present, it has attracted extensive attention in the field of biomedicine, especially as a carrier with excellent properties. TDNs are DNA nanomaterials with a three-dimensional structure formed by self-assembly of four specially designed nonsense sequences of single-stranded DNA (ssDNA) S1, S2, S3, and S4. The four single-stranded sequences follow the "principle of complementary base pairing" , the synthesis method is simple and the yield is high. It has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K31/7088A61P31/04
CPCA61P31/04A61K31/711A61P31/02A61K47/26Y02A50/30
Inventor 林云锋张雨欣蔡潇潇马文娟石思容
Owner SICHUAN UNIV
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