PH-induced drug sustained-release deoxyribonucleic acid (DNA) nanostructure as well as preparation method and application thereof

A nanostructure and DNA molecule technology, applied in DNA preparation, biochemical equipment and methods, recombinant DNA technology, etc., can solve problems such as influence and side effects, and achieve the effect of high pH response sensitivity, rapid response, and increased drug loading.
CN109675049AInactive Publication Date: 2019-04-26QINGDAO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO UNIV
Publication Date
2019-04-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The disclosure relates to a pH-induced drug sustained-release deoxyribonucleic acid (DNA) nanostructure as well as a preparation method and application thereof. Long single-stranded DNA is synthesizedby using a rolling circle amplification technology, and is complemented and hybridized with G and C-rich DNA single strand (loading strand) so as to obtain DNA molecular aggregates with alternating single and double strands; the DNA molecular aggregates can carry a large amount of Dox. When the pH of the system decreases, the rolling circle amplification product is folded to form a triple helix configuration, the double strands of the DNA molecule aggregates are melted, the G and C-rich DNA single strand is released, and the inserted Dox is also released, so that the drug release is completed. The reversible intercalation and release of the Dox can be achieved by adjusting the pH. The method utilizes the DNA nanostructure with good biocompatibility as a drug carrier to increase the drug loading amount, and has the advantages of being low in cost, simple to operate, high in sensitivity to pH response, rapid in response, and the like.
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Description

technical field

[0001] The disclosure relates to a pH-induced drug slow-release DNA nanostructure and its preparation method and application. Background technique

[0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art.

[0003] Chemotherapy is the main way to treat cancer at present, but chemotherapy drugs will spread throughout most of the tissues and organs of the whole body along with the blood circulation. Therefore, one of the disadvantages of chemotherapy is that while anticancer drugs attack tumor cells, they also affect non-pathological normal cells and cause serious side effects. For example, doxorubicin (Dox), an anthracycline widely used in cancer chemotherapy, can induce cardiomyopathy leading...

Claims

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