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Impedance type aptamer sensor for detecting lead ions based on gold nanomaterial and dendritic DNA nanostructure

An aptamer sensor and nanostructure technology, which is applied to the analysis of materials, electrochemical variables of materials, and material analysis through electromagnetic means, can solve the problems of high detection costs, expensive instruments, and high technical requirements for operators, and achieve repeatability. Good performance, fast response and high sensitivity

Pending Publication Date: 2022-06-03
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection methods of lead ions mainly include atomic absorption spectrometry, atomic fluorescence spectrometry, electrochemical anodic stripping voltammetry, etc. Although these methods have high sensitivity and accuracy, they require expensive instruments and high detection costs. The technical level of personnel is relatively high, which is not suitable for on-site inspection

Method used

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  • Impedance type aptamer sensor for detecting lead ions based on gold nanomaterial and dendritic DNA nanostructure
  • Impedance type aptamer sensor for detecting lead ions based on gold nanomaterial and dendritic DNA nanostructure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Specific steps are as follows:

(1) Formation of Y-DNA structure: Accurately pipette 2.5 μL of each of the three ssDNA (m, n, q) of 1 μmol / L into a 200 μL centrifuge tube, vortex to mix well, and set the PCR machine to 95 °C for 5 min, then slowly cooled to 4 °C and held for 30 min to complete the annealing procedure to form a Y-DNA structure.

[0016] (2) The construction of impedance-type aptamer sensor includes two parts: Y-DNA structure system and electrode system. Electrode system: Incubate 5 μL of gold nanoflower material on the electrode surface for 2 h, and then bind 5 μL of complementary strand, 2 μmol / L cDNA to the electrode surface in the form of Au-S for 2 h. When the sample contains lead ions, the aptamer chain Apt binds to the lead ions, so that the anchorDNA, the H1-H2 mixed solution of the hairpin and the Y-DNA structure are combined with the complementary strand cDNA on the electrode at a concentration ratio of 1 / 1 / 1. Finally, Rinse the electrode with phospha...

Embodiment 2

Specific steps are as follows:

(1) Formation of Y-DNA structure: Accurately pipette 2.5 μL of each of the three ssDNA (m, n, q) at 2 μmol / L into a 200 μL centrifuge tube, vortex to mix well, and set the PCR machine to 100 °C for 10 min, and then slowly cooled to 10 °C for 45 min to complete the annealing procedure to form a Y-DNA structure.

[0019] (2) The construction of impedance-type aptamer sensor includes two parts: Y-DNA structure system and electrode system. Electrode system: 7 μL of gold nanorod material was incubated on the electrode surface for 1.5 h, and then 7 μL of complementary strand, 4 μmol / L cDNA was bound to the electrode surface in the form of Au-S for 1.5 h. When the sample contains lead ions, the aptamer chain Apt binds to the lead ions, so that the anchor DNA, the hairpin H1-H2 mixed solution and the Y-DNA structure are combined with the complementary strand cDNA on the electrode at a concentration ratio of 1 / 2 / 2. Finally rinse the electrode with phtha...

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Abstract

The invention relates to an impedance type aptamer sensor for detecting lead ions based on a gold nanomaterial and a dendritic DNA nanostructure, which mainly comprises two parts: a Y-DNA structure system, and three ssDNA (m, n, q) are hybridized in pairs through complementary base pairing to form a Y-DNA structure. According to the electrode system, a gold nanomaterial is incubated to the surface of the electrode, and then the cDNA with complementary aptamer chains is combined to the surface of the electrode in an Au-S mode. When the sample contains lead ions, the aptamer chain Apt is combined with the lead ions, so that the anchor DNA, the hairpin H1 and H2 mixed solution and the Y-DNA structure are combined with the complementary chain cDNA on the electrode, and finally, the electrode is washed with a cleaning buffer solution. At the moment, the negative phosphoric acid skeleton in the dendritic double-chain structure on the electrode has a repulsive effect on the [Fe (CN) 6] 3- / 4-, so that the [Fe (CN) 6] 3- / 4-is effectively prevented from entering the surface of the electrode to cause the change of a response signal. Therefore, the impedance type aptamer sensor based on the gold nanomaterial and the dendritic DNA nanostructure is obtained for detecting lead ions. Compared with a traditional lead ion detection method, the method has the advantages of being high in response speed, high in sensitivity and good in repeatability.

Description

technical field [0001] The invention relates to an impedance-type aptamer sensor based on gold nanomaterials and dendritic DNA nanostructures to detect lead ions, in particular to a preparation method for combining complex DNA nanostructures. Background technique [0002] With the increase of industrial activities, heavy metal ions will inevitably be released into the environment, making the problem of heavy metal pollution increasingly serious. Among them, Pb is one of the most toxic heavy metal environmental pollutants. When it is discharged into the environment, it will be released as Pb 2+ As well as the existence of chemical compounds, they mainly enter the soil and water, are not degradable, and finally enter the human body through bioaccumulation, posing a threat to the ecosystem and human health. Pb 2+ It can be absorbed through the skin, accumulated in the digestive tract or food chain in soft tissues, especially in the liver, kidneys and brain, causing damage to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48Y02A50/30
Inventor 金华丽董洁郭蕊索志光孙晓霞程玉龙肖薇何保山
Owner HENAN UNIVERSITY OF TECHNOLOGY
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