Preparation method of DNA targeting nano medicine-carrying molecule for brain tumor
A DNA-targeting, nano-drug-loading technology, applied in anti-tumor drugs, pharmaceutical formulations, inactive components of polymer compounds, etc., can solve the problems of unseen patents, high penetration rate of brain malignant glioma, and insufficient drug treatment efficiency and other problems, to achieve the same size and structure, avoid damage, and overcome the effects of multi-drug resistance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] figure 1 Schematic diagram for the construction of the DNA-targeted nano-drug-carrying molecules, each take 2 μL of single-stranded DNA (50 μM) TSP-1, TSP-2, TSP-3, N 3 -TSP-4 was added to 42μL Tris-MgCl (Tris 10mM, MgCl 2 50mM, pH8) solution. The mixed solution was placed in a PCR instrument at a reaction temperature of 95° C., cooled rapidly to 4° C. after 10 minutes, and continued to react for 30 minutes. A single strand of DNA can self-assemble into a three-dimensional DNA tetrahedral configuration through complementary base pairing.
[0039] Take 180 μL of PBS solution (pH7.3), 40 μL of CuSO 4Aqueous solution (0.1 mM), 40 μL of TCEP aqueous solution (0.1 mM) and 40 μL of TBTA solution (10 μM, dissolved in DMSO) were prepared as a reaction solution. Take 100 μL of the prepared TDN solution (2 μM) and 200 μL of the target peptide solution (2 μM) whose amino acid sequence is RGERPPR, and add them to the above reaction solution, shake at 37°C for 5 hours at a const...
Embodiment 2
[0042] figure 1 Schematic diagram for the construction of the DNA-targeted nano drug-carrying molecules, each take 2 μL of single-stranded DNA (50 μM) TSP-1, TSP-2, Cy3-TSP-3, N 3 -TSP-4 was added to 42μL Tris-MgCl (Tris 10mM, MgCl 2 50mM, pH8) solution. The mixed solution was placed in a PCR instrument with a reaction temperature of 95° C., cooled to 4° C. after 10 minutes, and the reaction was continued for 30 minutes. The DNA single strand can self-assemble into a DNA tetrahedral three-dimensional configuration with a Cy3 fluorescent signal through complementary base pairing.
[0043] Take 180 μL of PBS solution (pH7.3), 40 μL of CuSO 4 Aqueous solution (0.1 mM), 40 μL of TCEP aqueous solution (0.1 mM) and 40 μL of TBTA solution (10 μM, dissolved in DMSO) were prepared as a reaction solution. Take 100 μL of the prepared TDN solution (2 μM) and 200 μL of the target peptide solution (2 μM) whose amino acid sequence is RGERPPR, and add them to the above reaction solution, ...
Embodiment 3
[0046] figure 1 Schematic diagram for the construction of the DNA-targeted nano-drug-carrying molecules, each take 2 μL of single-stranded DNA (50 μM) TSP-1, TSP-2, FAM-TSP-3, N 3 -TSP-4 was added to 42μL Tris-MgCl (Tris 10mM, MgCl 2 50mM, pH8) solution. The mixed solution was placed in a PCR instrument with a reaction temperature of 95° C., cooled to 4° C. after 10 minutes, and the reaction was continued for 30 minutes. DNA single strands can self-assemble into DNA tetrahedral three-dimensional configuration with FAM fluorescent signal through complementary base pairing.
[0047] Take 180 μL of PBS solution (pH7.3), 40 μL of CuSO 4 Aqueous solution (0.1 mM), 40 μL of TCEP aqueous solution (0.1 mM) and 40 μL of TBTA solution (10 μM, dissolved in DMSO) were prepared as a reaction solution. Take 100 μL of the prepared TDN solution (2 μM) and 200 μL of the target peptide solution (2 μM) whose amino acid sequence is RGERPPR, and add them to the above reaction solution, shake a...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap