Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

102results about How to "Effective embedding" patented technology

Method for extracting DHA from cells of algae and fungi by breaking cell walls

The invention provides a method for extracting DHA from cells of algae and fungi by breaking cell walls and relates to a method for extracting active ingredients from microorganisms. The method provided by the invention avoids the assistance from any organic solvent and chemical medicaments, breaks the walls of the cells of the algae and the fungi by using a physical method and extracts a grease product DHA (docosahexaenoic acid) from the cells and is suitable for large-scale production at low cost. The method comprises: after fermentation is finished, introducing fermentation liquor of microalgae or fungi to a separation system for separating and collecting the cells, adjusting the pH value of bacterial sludge with an acid to 2.0 to 4.0, keeping the temperature of the bacterial sludge between 10 and 20 DEG C, adding antioxidant into the bacterial sludge, and performing high-pressure homogenization and wall breaking by using a high-pressure homogenizer; and adding water into the bacterial sludge subjected to wall breaking, stirring the bacterial sludge, and separating feed liquor in a three-phase separator to obtain DHA grease. In the invention, physical wall breaking and the physical extracting method are used, the process is simple, the cell breaking is high efficient, the low-temperature treatment and antioxidant treatment of the bacterial sludge can effectively protect thebioactivities of the matters in the cells of the algae and the fungi, and the product is green, nontoxic and free from residues.
Owner:XIAMEN HUISON BIOTECH

Method for producing water-soluble functional red koji powder

The invention discloses a production method of water-soluble functional monascus powder, which belongs to the technical fields of biological engineering and health product preparation. In the invention, the water-soluble functional monascus powder containing a Monacolin K is prepared the following steps of: taking a high-yielding Monacolin K purple monascus without a notalin as a starting bacterium, carrying out solid state fermentation of corns, then extracting, condensing, adjusting the pH, adding cyclodextrine and embedding a water-soluble corn hydrolysate and spray drying; or the corn is fermented in a liquid state and then a fermentation liquid is manufactured into the water-soluble functional monascus powder containing the Monacolin K through removing a thalli by filtering, adjusting the pH, adding the cyclodextrine and embedding the water-soluble corn hydrolysate and spray drying; and detail researches are carried out on the technical conditions of fermenting, extracting, embedding and spray drying, thus obtaining the optimized technical parameters; the product has excellent water-solubility; the Monacolin K content in the monascus powder product can achieve 20g/kg; the Monacolin K proportion of an open ring structure can achieve more than 90 percent. The water solution which is prepared by the water-soluble functional monascus powder product of 20 percent can maintain clear and transparent under the room temperature, does not contain deposition and has better stability.
Owner:JIANGNAN UNIV

Synaptic transistor based on two-dimensional semiconductor material and preparation method of synaptic transistor

The invention discloses a synaptic transistor based on a two-dimensional semiconductor material and a preparation method of the synaptic transistor. The synaptic transistor comprises an insulating substrate, and a channel, a source electrode, a drain electrode and a gate electrode which are arranged on the substrate, wherein the channel is a two-dimensional semiconductor material; the source electrode and the drain electrode are arranged at the two ends of the channel respectively and form an ohmic contact with the channel material; the gate electrode and an electrical interconnection system formed by the channel, the source electrode and the drain electrode are kept in electronic insulation; an organic electrolyte covers a channel region and most of the gate electrode and comprises an organic carrier capable of being electrically insulated and ions capable of being migrated, and effective ion control of the gate to the channel material is formed. According to the synaptic transistor based on the two-dimensional semiconductor material and the preparation method of the synaptic transistor, an ion attachment-intercalation mechanism is utilized, and the characteristics of large surface area and adjustable resistance value of the two-dimensional material are combined, so that the device shows long-term and short-term synaptic plasticity, and the two characteristics can change witheach other along with the change of a gate signal. Meanwhile, the device has good linearity and ultralow operational power consumption, and the implementation and large-scale integration application of a high-precision neuromorphic device are facilitated.
Owner:PEKING UNIV

High-internal-phase emulsion of soybean protein isolate-pectin compound stabilized quercetin and preparation method of high-internal-phase emulsion

The invention belongs to the technical field of emulsions, and discloses a high-internal-phase emulsion of soybean protein isolate-pectin compound stabilized quercetin and a preparation method of thehigh-internal-phase emulsion. The method comprises the following steps: dissolving soybean protein isolate in distilled water to obtain a soybean protein isolate solution; dissolving pectin in distilled water, and adjusting the pH value to obtain a pectin solution; mixing the soybean protein isolate solution with the pectin solution; adjusting the pH value of the solution to 3.0-11.0; mixing the obtained solution with vegetable oil containing quercetin in proportion, then carrying out ultrasonic treatment, and carrying out centrifuging to obtain the high-internal-phase emulsion of soybean protein isolate-pectin compound stabilized quercetin. According to the invention, no surfactant is needed, and the obtained oil-in-water type emulsion is small in particle size, good in viscoelasticity and high in stability and has an excellent protection or controlled release effect on quercetin. The preparation method is low in instrument requirement, the method is simple and is easy to operate, andgood prospects are achieved in application aspects of novel nutrient substance conveying carriers and food structure modification base materials.
Owner:NANJING FORESTRY UNIV

Water-soluble perylene imide compounds, usage as DNA intercalator, and applications thereof in growth inhibition of cancer cells

The invention discloses water-soluble perylene imide compounds, a usage as a DNA intercalator, and applications thereof in the growth inhibition of cancer cells, and belongs to the technical field of chemical synthesis of biological drugs. Through inducing cation amino functional groups into perylene anhydride derivatives and a perylene mono-anhydride system, the water solubility of the perylene anhydride derivatives and the perylene mono-anhydride system is greatly improved; furthermore, the cation amino functional groups are in a photo-stability combination with perylene derivatives, and thus a series of novel water-soluble perylene imide compounds are obtained. The synthesized water-soluble perylene imide compounds can effectively be embedded between the base pairs of DNA double helix of nucleus and be used as a DNA intercalator. The synthesized water-soluble perylene imide compounds have a planar rigid structure, so that the compounds can be specifically enriched around fixed tissue cell nucleus, the compounds have the advantages of strong fluorescence and easiness in fluorescent imaging, and have a similar effect as the DAPI effect of commercial cell nucleus dye. The synthesized water-soluble perylene imide compounds can effectively inhibit the growth of cancer cells such as U2OS, HTC116, Hela, AGS, etc., and have a very prominent anti-tumor effect.
Owner:BEIJING UNIV OF CHEM TECH

Water-soluble perylene bisimide compound and application of water-soluble perylene bisimide compound serving as DNA intercalator in anticancer cells and tumors

The invention discloses a water-soluble perylene bisimide compound and an application of a water-soluble perylene bisimide compound for preparing DNA intercalators, inhibiting cancer cell growth and resisting tumors, belonging to the technical field of biological drug chemical synthesis. A cationic amino functional group is introduced into perylene derivatives and single perylene systems, so that the water solubility is greatly improved, the amino functional group is bound to light stability of perylene derivatives, and a series of novel water-soluble perylene bisimide compounds are obtained. The synthetic compound can be effectively embedded into DNA double helix base pairs of the cell nucleus and can serve as a DNA intercalator. The synthetic compound has a planar rigid structure, can be specifically enriched on a fixed tissue cell nucleus, is high in fluorescence intensity and easy in fluorescence imaging and can be compared favorably with commercial cell nucleus dyes DAPI. The synthetic compound can effectively inhibit growth of cancer cells such as U2OS, HCT116, HeLa and AGS, has an obvious antitumor effect and can be applied to preparing antitumor drugs.
Owner:BEIJING UNIV OF CHEM TECH

Method for PVD film plating on surfaces of workpieces

The invention relates to a method for PVD film plating on surfaces of workpieces, belongs to the technical field of surface treatment, and solves the defects of weak product compactness and corrosionresistance caused by a certain defects in coating layers formed by single film plating technologies in traditional PVC technologies. The method for PVD film plating on the surfaces of the workpieces comprises the following steps: (A) precleaned workpieces to be plated are put in a film plating chamber; and then, the film plating chamber is vacuumized and heated; (B) after heating, argon is introduced for ion bombardment on the workpieces to be plated; and (C) working gas is introduced; a power supply is turned on to apply bias voltage to the workpieces to be plated; a target source is turned on to start film plating; then, the target source is driven by periodical change of an electric field and a magnetic field to switch between magnetron sputtering and cathode arc modes for film plating;and plating layers with corresponding materials are deposited on the surfaces of the workpieces to be plated to obtain the surface-plated workpieces. The method for PVD film plating on the surfaces of the workpieces can realize better film layer compact structures and efficient corrosion resistance.
Owner:SUZHOU CHAMPION COATING TECH

Slow-release fragrant gel composition and preparation method thereof

The invention discloses a slow-release fragrant gel composition and a preparation method thereof. The slow-release fragrant gel composition comprises 530 parts of 1000 polyethylene glycol, 540 parts of 1500 polyethylene glycol, 530 parts of 6000 polyethylene glycol, 110 parts of thickening agent, 1-10 parts of emulsifying agent, 1-10 parts of humectant and 5-30 parts of essence. The preparation method includes the steps that the 1000 polyethylene glycol, the 1500 polyethylene glycol and the 6000 polyethylene glycol are mixed according to a proportion and melt at the temperature of 50-60 DEG C,and then a mixture A is obtained; the essence and the emulsifying agent are uniformly mixed according to a proportion, so that a mixture B is obtained; the mixture B is placed into a reaction kettleat the temperature of 40 DEG C or below, emulsifying is performed for 30 minutes at the rotating speed of 45 r / min, the mixture B after emulsifying is slowly added into the mixture A, then the humectant and the thickening agent are added, and finally the slow-release fragrant gel composition is obtained after uniform stirring. The gel composition is good in stability, the content of fragrant in agel component is increased greatly, and vaporization of fragrant substances during preparation is small. The main aim is to solve the problem that in traditional gel of the prior art, a formed filterstick is prone to seam slippage because of oil-soluble essence.
Owner:CHINA TOBACCO YUNAN NEW MATERIAL

Nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as preparation method and application thereof

The invention discloses a nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as a preparation method and application thereof. The method comprises the following steps of adding sodium alginate in a nano-crystalline cellulose solution, uand performing uniform mixing and centrifuging to obtain a mixed solution; adding chitosan in water, performing uniform mixing, and adding glacial acetic acid to obtain a chitosan solution; adding lactobacillus powder in the chitosan solution, and performing uniform mixing to obtain a chitosan-lactobacillus bacterial suspension; taking the mixed solution as an outer material, and the chitosan-lactobacillus bacterial suspension as an inner material, and performing 3D printing to obtain a printing product; and spraying the surface of the printing product with a calcium chloride solution, and performing curing and cold drying to obtain the nano-crystalline cellulose based 3D printing lactobacillus embedding material. For the nano-crystalline cellulose based 3D printing lactobacillus embedding material, the lactobacillus is immobilized by taking the nano-crystalline cellulose and the chitosan as carriers, so that efficient continuous cyclic fermentation of yogurt can be realized, and the problems of difficulty in preservation of lactobacillus, low utilization rate and high cost can be solved.
Owner:SOUTH CHINA UNIV OF TECH

Method for constructing porous structure on surface of polyether ether ketone scaffold

The invention relates to a method for constructing a porous structure on a surface of a polyether ether ketone scaffold. The method mainly comprises the following steps: A, preparing hard particles, concretely pulverizing a hard material, and carrying out sieving to obtain hard particles with a particle size of 200-800 microns; B, embedding the hard particles, concretely adhering the hard particles to the surface of the polyether ether ketone scaffold, placing the polyether ether ketone scaffold in a hot press, then carrying out heating to 170-190 DEG C, keeping the temperature for 1.5-2.5 h,and then applying pressure of 2-4Mpa to obtain the polyether ether ketone scaffold with the hard particles embedded on the surface; and C, carrying out acid etching treatment, concretely placing the polyether ether ketone scaffold prepared in the step B in a hydrofluoric acid solution for 44-52h, removing the hard particles by etching, and then carrying out cleaning to obtain the porous structure.The number and size of the porous structures obtained on the surface of the polyether ether ketone scaffold by the method can be controlled. After the scaffold is implanted into a human body, growthof bone tissues into the scaffold is facilitated, and bone integration performance is good. In addition, mechanical performance is good, and the scaffold structure is stable.
Owner:SOUTHWEST JIAOTONG UNIV

Intestinal probiotic powder for abalone culture and preparation method thereof

InactiveCN102578402AHigh pertinence and stabilityPromote growthAnimal feeding stuffFermentation brothAgar
The invention discloses intestinal probiotic powder for abalone culture and a preparation method thereof and relates to a live bacteria preparation for abalone culture. The intestinal probiotic powder comprises probiotic dry powder, agar powder and feed. The preparation method comprises the following steps of: respectively activating Phaeobacter sp. AP1220 and Bacillus stratosphericus A3440, which are separated from abalone intestines via a culture medium, and then inoculating the Phaeobacter sp. AP1220 and the Bacillus stratosphericus A3440 into a fermentation culture medium for shaking culture so as to enable the concentration of thalli to achieve logarithmic phase 0.5-1.0 multiplied by 10<9>cfu/ml; mixing all strain fermentation solutions, then centrifugating, abandoning supernatant fluid, and performing precipitation, washing, centrifugation and sterilization on the thalli to get bacterial sludge; preparing a dry powder protecting agent; mixing the dry powder protecting agent withthe bacterial sludge, performing a film-forming reaction, then pre-freezing a mixture and further performing freeze-drying to prepare the active probiotic dry powder with an embedding layer; and mixing the probiotic dry powder, the agar powder and the feed to prepare the intestinal probiotic powder for abalone culture.
Owner:XIAMEN UNIV

Lycium ruthenicum extract micro-capsule and lycium ruthenicum preparations prepared from same

The invention relates to a lycium ruthenicum extract micro-capsule and lycium ruthenicum preparations prepared from the same. According to the lycium ruthenicum extract micro-capsule, lycium ruthenicum extract is dispersed into capsule materials to form spray liquid, and then the spray liquid is processed through a spray drying method or a spray condensing method to obtain the lycium ruthenicum extract micro-capsule. The lycium ruthenicum extract micro-capsule achieves micro-encapsulation of the prepared lycium ruthenicum extract and improves the bitter taste of lycium ruthenicum, particularly the hygroscopic problem caused by polysaccharide components, thereby improving the quality stability of lycium ruthenicum products, prolonging the shelf life, brining convenience to production, transportation and storage of the lycium ruthenicum products and facilitating large-scale industrial production. Meanwhile, through appropriate granulating processes, micro-encapsulated lycium ruthenicum extract dry powder can be processed into a granulated solid applicable to multiple preparation forms. The lycium ruthenicum extract micro-capsule and the lycium ruthenicum preparations prepared from the same achieve the effects of enhancing body immunity, clearing liver, improving eyesight, delaying senescence, improving sleep and resisting fatigue.
Owner:NINGXIA MEDICAL UNIV

Preparation method of composite probiotic ice cream

The invention relates to a preparation method of a composite probiotic ice cream. The preparation method comprises the following steps of (1) weighing raw materials according to the formula: whole milk powder, soybean milk powder, water, cane sugar, beta-cyclodextrin, soybean protein powder, inulin, honeysuckle flower powder, Chinese wolfberry fruit powder and liquiritigenin for standby application; and (2) uniformly mixing and stirring the weighed raw materials in step (1), then performing homogenizing, performing sterilization and performing ageing to obtain an ice cream mixture, then adding0.003-0.008 part of composite probiotics to the ice cream mixture, performing uniform stirring to obtain a mixture, sending the mixture to a condensing and freezing machine for condensing and freezing, after condensing and freezing, performing filling, and performing hardening, so as to obtain the probiotic ice cream. The ice cream made by the preparation method is rich in nutrition, various probiotics and traditional Chinese medicine components, and has good health-care functions. According to the method, the ice cream is compounded from specific components in specific parts by weight, so that the survival rate of probiotics for the probiotic ice cream in the preparation and preservation process can be increased, and normal exertion of the health-care functions of the probiotic ice creamcan be guaranteed.
Owner:CHENGDU TIAN HANG ZHI HONG IP MANAGEMENT CO LTD

Overall assembly type reinforcement printing construction method for 3D printing concrete structure

The invention discloses an overall assembly type reinforcement printing construction method for a 3D printing concrete structure. The method comprises the steps that a structural member is selected, and a reinforcement material is determined according to the stress distribution and magnitude in the bearing force limit state and the stress strength ratio in the normal use bearing force state; the positions, number and forms of connecting sleeves are determined according to the joint point positions and the number of intersections of the reinforcement material; the printing process of concrete, the reinforcement material and the connecting sleeves is determined according to the spatial appearance of the structural member, and the timing positioning connection and pushing program of the reinforcement material and the connecting sleeves is edited according to the printing process; and a concrete layer is formed along the printing process, the reinforcement material and the connecting sleeves are placed into the unhardened concrete layer according to the timing positioning connection and pushing program and assembled, and the 3D printing concrete structure with the spatial multi-directional connecting sleeves integrally reinforced is formed. The 3D printing concrete structure prepared through the construction method forms a spatial rigidity-enhanced framework, and the spatial rigidity-enhanced framework is close to a steel reinforcement framework formed through a traditional construction method.
Owner:ZHEJIANG UNIV

Silicon-carbon negative electrode material and preparation method thereof

The invention discloses a silicon-carbon negative electrode material with a stable structure and good cycle performance and a preparation method of the silicon-carbon negative electrode material. The stable structure specifically refers to the stable internal structure of silicon nanoparticles and the stable structure of silicon-carbon particles formed by compounding the silicon nanoparticles, carbon nanotubes, graphite nanosheets and amorphous carbon. The silicon nanoparticles are high in amorphization degree and small in grain size, and the interior of the silicon nanoparticles is of a structure in which nanocrystals are uniformly embedded in an amorphous state. According to the silicon-carbon composite particles, a multi-layer frame structure is built through the graphite nanosheets, the multi-layer frame structure and the uniformly dispersed carbon nanotubes form a supporting structure; the silicon nanoparticles are in close contact with the carbon nanotubes and the graphite nanosheets to form dense composite particles; and the outer layers of the composite particles are coated with a layer of dense amorphous carbon material. The silicon-carbon negative electrode material is small in specific surface area (less than 4m < 2 >/g), high in compaction density, high in specific capacity and good in cycle performance (the capacity retention rate is as high as 86% after a whole battery undergoes 900 cycles at normal temperature).
Owner:江苏载驰科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products