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Novel nanoparticles with core-shell structures and preparation method thereof

A core-shell structure and nanoparticle technology, applied in the fields of nanobiology and nanopharmaceuticals, can solve problems such as the influence of biological modification, and achieve the effect of stable properties and good stability

Active Publication Date: 2017-10-13
SHANDONG BOAOKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The surface of the gold-silver core-shell structure prepared by the current method is covered with a thin layer of silver, which will affect some subsequent biological modifications.

Method used

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  • Novel nanoparticles with core-shell structures and preparation method thereof
  • Novel nanoparticles with core-shell structures and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of novel core-shell structured nanoparticles

[0023] The preparation process is as figure 1 As shown, the steps are as follows:

[0024] (1) Preparation of nano gold rods: at room temperature, 10ml of 0.1mol L -1 The CTAB solution was added to the container (20ml round bottom flask), and then 50ul of 50mmol·L -1 HAuCl 4 solution, stirred rapidly for 1 minute; then quickly added 0.6ml of 10mmol·L -1 NaBH 4 The solution was stirred rapidly for 1 minute to obtain seed crystals;

[0025] 30ml of 0.1mol·L -1 Add the CTAB solution in another container (50ml round bottom flask), stir at an appropriate speed (add while stirring, to mix evenly), add 234ul of 50mmol L -1 HAuCl 4 solution, and then add 234ul of 10mmol·L -1 Silver nitrate solution, then add 150ul of 0.1mol L -1The L-AA solution (ascorbic acid solution), now the solution changed from light yellow to colorless, finally added 90ul seed crystals, stirred for 1 minute, and left to stand...

Embodiment 2

[0032] Example 2 Preparation of novel core-shell structured nanoparticles

[0033] Proceed as follows:

[0034] (1) Preparation of nano gold rods: at room temperature, 15ml of 0.1mol L -1 The CTAB solution was added to the reaction vessel (20ml round bottom flask), and then 80ul of 50mmol L -1 HAuCl 4 solution, stirred rapidly for 1 minute; then quickly added 1.0ml of 10mmol·L -1 NaBH 4 The solution was stirred rapidly for 1 minute to obtain seed crystals;

[0035] 40ml of 0.1mol·L -1 The CTAB solution was added to another reaction vessel (50ml round bottom flask), under stirring at an appropriate speed (adding while stirring, to mix evenly), add 250ul of 50mmol L -1 HAuCl 4 solution, and then add 250ul of 10mmol·L -1 Silver nitrate solution, then add 180ul of 0.1mol L -1 The L-AA solution (ascorbic acid solution), now the solution changed from light yellow to colorless, finally added 90ul seed crystals, stirred for 1 minute, and left to stand for 4 hours, the solutio...

Embodiment 3

[0039] Example 3 Preparation of novel core-shell structured nanoparticles

[0040] Proceed as follows:

[0041] (1) Preparation of nano gold rods: at room temperature, 12ml of 0.1mol L -1 The CTAB solution was added to the reaction vessel (20ml round bottom flask), and then 60ul of 50mmol L -1 HAuCl 4 solution, stirred rapidly for 1 minute; then quickly added 0.8ml of 10mmol·L -1 NaBH 4 The solution was stirred rapidly for 1 minute to obtain seed crystals;

[0042] 35ml of 0.1mol·L -1 The CTAB solution was added to another reaction vessel (50ml round bottom flask), under stirring at an appropriate speed (adding while stirring, to mix evenly), add 240ul of 50mmol L -1 HAuCl 4 solution, and then add 240ul of 10mmol·L -1 Silver nitrate solution, then add 170ul of 0.1mol L -1 The L-AA solution (ascorbic acid solution), now the solution changed from light yellow to colorless, finally added 90ul seed crystals, stirred for 1 minute, and left to stand for 4 hours, the solutio...

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Abstract

The invention discloses novel nanoparticles with core-shell structures. A preparation method comprises the following steps: (1) adding CTAB solution into a container, adding HAuCl4 solution, and then adding NaBH4 solution to obtain seed crystal; adding the CTAB solution into another container, sequentially adding the HAuCl4 solution, silver nitrate solution, L-AA solution and the seed crystal to prepare gold nanorods; (2) carrying out DNA modification on the gold nanorods to obtain gold nanorods stably protected by thiolated DNA; and (3) centrifuging the gold nanorods, dissolving the gold nanorods in the CTAB solution, sequentially adding SDS solution, the silver nitrate solution and the L-AA solution, and standing to obtain the novel nanoparticles with the core-shell structures. The nanoparticles disclosed by the invention are high in stability, thin silver covers the two ends of each core-shell structure, most of each connected DNA single strand is exposed outside, and each tip part has a higher surface plasma effect; and the nanoparticles have a high application value in the fields of bio-optoelectronics, biological nanometer detection and the like.

Description

technical field [0001] The invention relates to a novel core-shell structure nanoparticle and a preparation method thereof, belonging to the fields of nanobiology and nanopharmaceuticals. Background technique [0002] Gold was first extracted as a heavy metal material in the 5th century BC, and it became even more precious when it was divided into gold nanoparticles with a size below 100nm. Gold nanoparticles refer to tiny particles of gold with a diameter of 1-100 nm. Gold nanoparticles have high electron cloud density, dielectric properties and catalytic effects, and have good biocompatibility. A variety of biomacromolecules can be combined without affecting their biological activity. Gold nanoparticles with diameters at the nanometer level, the basic units are tiny particles, which have many physical properties that macroscopic gold does not have, such as optical effects, surface effects, small size effects, dielectric confinement effects, macroscopic quantum tunneling e...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F1/02B22F9/24B82Y40/00
CPCB82Y40/00B22F9/24B22F1/102B22F1/054
Inventor 石党委翟逸郭宝柱赵秀敏吴永坤刘钦松
Owner SHANDONG BOAOKE BIOTECH
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