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Staphylococcus lentus selective medium and preparation method thereof

A staphylococcus and selective technology, applied in the field of medical testing, can solve the problems such as the inability to selectively cultivate Staphylococcus lentus, the inability to grow Staphylococcus lentus, etc., and achieve the effects of reliable detection and high separation rate.

Inactive Publication Date: 2013-11-13
董彦军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this medium cannot selectively cultivate Staphylococcus lentus, and the reason is that Staphylococcus aureus grows faster than Staphylococcus lentus in the current selective medium, covering the medium quickly, and Staphylococcus lentus cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; add distilled water to 1000ml. Preparation method: take malt extract powder; egg protein liquid; sodium chloride; magnesium citrate; agar; After filtration, distilled water was used to make up to volume, sterilized twice at low temperature, each time for 45 min, and then packaged after sterilization.

Embodiment 2

[0018] Example 2, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; copper sulfate 5mg; distilled water added to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; , mixed and filtered, distilled water to volume, low-temperature sterilization twice, 45 min each time, after sterilization, aliquoted.

Embodiment 3

[0019] Example 3, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; L-cysteine 0.1 g; add distilled water to 1000ml. Preparation method: extract malt powder; egg white liquid; sodium chloride; magnesium citrate; agar; Dissolve in distilled water, mix and filter, distilled water to volume, sterilize twice at low temperature, 45 min each time, and pack after sterilization.

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Abstract

The invention discloses a staphylococcus lentus selective medium and a preparation method thereof. The staphylococcus lentus selective medium is characterized in that the formula of 1000 ml of selective medium comprises the following components by weight: 15g of malt extract, 150ml of egg protein fluid, 5g of sodium chloride, 0.5g of magnesium citrate, 15g of agar, 8-12mg of coetsoidin, 3.6g of N-acetyl-D-glucosamine and 0.2g of monopotassium phosphate, which are prepared to 1000 ml by adding distilled water. The optimal preparation method for the staphylococcus lentus selective medium comprises the following steps of: dissolving the malt extract, the egg protein fluid, sodium chloride, magnesium citrate, agar, coetsoidin, N-acetyl-D-glucosamine, monopotassium phosphate, L-cysteine and copper sulfate in lily decocted liquid; uniformly mixing the components, metering the volume of the mixture, sterilizing, splitting and packaging the mixture. Compared with the prior art, the preparation method has the characteristics that the detection is reliable, and the separation rate of the staphylococcus lentus is high.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a selective culture medium for Staphylococcus lentus and a preparation method thereof. Background technique [0002] Staphylococcus lentus belongs to the genus Staphylococcus. The bacterium was divided into an independent species of Staphylococcus by Schleifer K H et al. in 1983, that is, Staphylococcus lentus (S.lentus). Staphylococcus lentus was once considered non-pathogenic, but more and more data show that S.lentus is a pathogenic bacteria that cannot be ignored in humans or many animals, it can cause human diseases and play an important role in nosocomial infections, especially for Immunocompromised groups such as newborns, patients with serious underlying diseases or patients receiving long-term hospital treatment are more likely to cause serious consequences. Due to the increasingly serious problem of multi-drug resistance of S.lentus, the infection caused by S.lentus sho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/14
Inventor 董彦军
Owner 董彦军
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