Solid culture medium suitable for co-growing Candida albicans and staphylococcus

A technology of Candida albicans and solid medium, which is applied in the field of medicine, can solve the problems of growth status and sensitivity effects, and achieve the effect of good growth status, rich nutrition, and simple preparation method

Inactive Publication Date: 2012-06-27
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the two are cultured in different media, due to the nutrient limitation of the media, their growth status and sensitivity will be affected.
[0007] In existing studies (Pate JC, Jones DB and Wilhelmus KR. Prevalence and spectrum of bacterial co-infection during fungal keratitis. Br. J. Ophthalmol. 2006, 90: 289-292), there have been mixed Research on the biofilm morphology and the change of signal molecules of fungi and bacteria in BF, but there is no observation experiment on the morphology and drug effect of mixed bacteria on solid medium

Method used

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  • Solid culture medium suitable for co-growing Candida albicans and staphylococcus
  • Solid culture medium suitable for co-growing Candida albicans and staphylococcus
  • Solid culture medium suitable for co-growing Candida albicans and staphylococcus

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0029] Example 1. Screening of solid medium components for mixed culture of fungi and bacteria

[0030] 1. Experimental materials

[0031] 1.1 strains:

[0032] The identified Candida albicans CA1 was clinically isolated from Qianfoshan Hospital of Shandong Province and identified by Shandong Health and Epidemic Prevention Station. Staphylococcus aureus SA10, Staphylococcus epidermidis SE9, courtesy of Microbiology Laboratory of Shandong Academy of Medical Sciences.

[0033] 1.2 Reagents:

[0034] Yeast extract, peptone, glucose, NaCl, beef powder, agar powder were purchased from Dingguo Biotechnology Co., Ltd.;

[0035] Anhydrous calcium chloride, Tianjin Bodi Chemical Co., Ltd., made 4.5mmol / L stock solution with sterile distilled water, standby;

[0036] Phosphate buffer saline (PBS): Weigh KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 1.44g, NaCl 0.2g, KCl 8g, adjust pH to 7.4, constant volume 1L, steam sterilize, store at room temperature.

[0037] Sheep blood: a gift fr...

example 2

[0057] Example 2. Effect of sheep blood on the growth of bacterial strains

[0058] 1. Experimental materials

[0059] 1.1 strains:

[0060] The identified Candida albicans CA1 was clinically isolated from Qianfoshan Hospital of Shandong Province and identified by Shandong Health and Epidemic Prevention Station. Staphylococcus aureus SA10, Staphylococcus epidermidis SE9, courtesy of Microbiology Laboratory of Shandong Academy of Medical Sciences.

[0061] 1.2 Reagents:

[0062] Yeast extract, peptone, glucose, NaCl, beef powder, agar powder were purchased from Dingguo Biotechnology Co., Ltd.;

[0063] Anhydrous calcium chloride, Tianjin Bodi Chemical Co., Ltd., made 4.5mmol / L stock solution with sterile distilled water, standby;

[0064] Phosphate buffer saline (PBS): Weigh KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 1.44g, NaCl 0.2g, KCl 8g, adjust pH to 7.4, constant volume 1L, steam sterilize, store at room temperature.

[0065] 2. Experimental method

[0066] The prepa...

example 3

[0069] Example 3. Ca 2+ Effect on strain growth experiment

[0070] 1. Experimental materials

[0071] 1.1 strains:

[0072] The identified Candida albicans CA1 was clinically isolated from Qianfoshan Hospital of Shandong Province and identified by Shandong Health and Epidemic Prevention Station. Staphylococcus aureus SA10, Staphylococcus epidermidis SE9, courtesy of Microbiology Laboratory of Shandong Academy of Medical Sciences.

[0073] 1.2 Reagents:

[0074] Yeast extract, peptone, glucose, NaCl, beef powder, agar powder were purchased from Dingguo Biotechnology Co., Ltd.;

[0075] Sheep blood: a gift from the Department of Infectious Diseases, Qianfoshan Hospital, Shandong Province.

[0076] Phosphate buffer saline (PBS): Weigh KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 1.44g, NaCl 0.2g, KCl 8g, adjust pH to 7.4, constant volume 1L, steam sterilize, store at room temperature.

[0077] 2. Experimental method

[0078] The preparation methods of the bacterial liquid an...

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Abstract

The invention discloses a culture medium for co-culturing Candida albicans and staphylococcus, and in particular relates to the influence of different nutritional components, calcium ions and blood on the growth of the Candida albicans and the staphylococcus and discussion on the proportion of a culture medium which is the most suitable for growing the Candida albicans and the staphylococcus. The culture medium comprises the following components in percentage by mass: 0.15 to 0.25 percent of yeast extract, 1.15 to 1.25 percent of peptone, 0.3 to 0.5 percent of glucose, 0.375 to 0.425 percent of NaCl, 0.25 to 0.255 percent of beef powder and 1.575 to 1.625 percent of agar powder. The Candida albicans and staphylococcus aureus/staphylococcus epidermidis are researched, and the influence of the culture medium on the size and shape of bacterial colonies of the Candida albicans and the staphylococcus aureus/staphylococcus epidermidis is directly evaluated by a streak plate method. Results show that the Candida albicans and the staphylococcus aureus/staphylococcus epidermidis can be well grown when the culture medium is adopted.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a solid medium suitable for the co-growth of Candida albicans and Staphylococcus. Background technique [0002] In recent years, with the increase in the incidence of malignant tumors, AIDS, and autoimmune diseases, the development of techniques such as catheter intubation and organ transplantation, and the widespread use of broad-spectrum antibiotics, corticosteroids, and immunosuppressants, the incidence of fungal infections has increased sharply. Rising (Georgopapadakou NH. Antifungals: mechanism of action and resistance, established and novel drugs [J]. Current Opinion in Microbiology, 1998, 1(5): 547-557.), especially the application of ventilators and a large number of biological materials, The incidence of fungal infection associated with biofilm (biofilm, BF) continues to increase. Biofilms are a common form of microbial life. A biofilm (bioflim, BF) is a structured gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/44C12R1/725
Inventor 孙淑娟高媛刘萍
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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