The invention discloses a making method of an animal model for imitating fungal infection of porcine cornea, composed of steps of preparing fungi strains, preparing inoculum, preparing animals, inoculating animals and processing after the inoculating. The invention has the advantages that model-making success rate is high, generally the success rate of outbred animals reaches over 85% and the success rate of inbred animals reaches over 90%, immunosuppressant is not necessary, fungal hyphaes are observed in pathological section of cornea among the second day and thirteenth day after inoculating, operational method is economical, simple and easy to apply, materials are easy to get, inoculating method is easy to master, researchers need no special technology, the invention is suitable for the process of clinical natural infection of fungal keratitis, and is adaptable for the research of the generation, development and the mechanism of infection.