Method for making animal model simulated human cornea fungal infection

Abstract

The invention discloses a making method of an animal model for imitating fungal infection of porcine cornea, composed of steps of preparing fungi strains, preparing inoculum, preparing animals, inoculating animals and processing after the inoculating. The invention has the advantages that model-making success rate is high, generally the success rate of outbred animals reaches over 85% and the success rate of inbred animals reaches over 90%, immunosuppressant is not necessary, fungal hyphaes are observed in pathological section of cornea among the second day and thirteenth day after inoculating, operational method is economical, simple and easy to apply, materials are easy to get, inoculating method is easy to master, researchers need no special technology, the invention is suitable for the process of clinical natural infection of fungal keratitis, and is adaptable for the research of the generation, development and the mechanism of infection.

Description

technical field [0001] The invention belongs to medical models, in particular to a method for making an animal model simulating human corneal fungal infection. Background technique [0002] Fungal keratitis is an infectious corneal ulcer with a high rate of blindness. With the wide application of antibiotics and glucocorticoids and the improvement of the understanding and diagnosis of this disease, its incidence rate has been increasing. Common pathogenic fungi include Fusarium, Aspergillus, Penicillium, and Candida. Most of the patients in our country are farmers, and they often occur after vegetative corneal trauma. At present, there are very few basic studies on corneal fungal infection, and its pathogenesis is unclear. The lack of animal models simulating human corneal fungal infection has seriously hindered the basic research of fungal keratitis. The following methods for making animal models of fungal keratitis are currently disclosed: [0003] 1. Corneal stromal inj...

AI Technical Summary

Problems solved by technology

In the experiment, the amount of fungus in the cornea decreased rapidly after inoculation in mice without immunosuppressive agents

In 2004, they used the same method to inoculate Fusarium solani and found that in the pathological sections, BALB / C mice without immunosuppressants could not be detected or occasionally had a small amount of fungal hyphae in the corneal stroma on the fourth day after inoculation. , so this method is based on making animals in a state of immunocompromise in advance, but most patients with fungal keratitis are not in an immunosuppressed state when they develop clinically. Easy to cause corneal perforation, not suitable for research on the occurrence, development and mechanism of infection

These two methods have a high success rate of infection, and both avoid the influence of hormones on the experimental results, but they do not conform to the clinical course of natural infection. The operation process of the method is relatively complicated, and there are certain requirements for the experimenter's surgical skills.