Medical material for treating fungal keratitis and preparation method thereof

A technology for fungal keratitis and enzyme-producing lysobacteria, which is applied in the field of medical materials, can solve the problems of general patient compliance and satisfaction, frequent eye drops, and low bioavailability, achieving good application prospects and Scientific value, simple equipment, and good biocompatibility

Pending Publication Date: 2021-05-11
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the drugs used clinically for the treatment of fungal keratitis are in the form of eye drops. The number of eye drops is frequently dispensed, the bioavailability is low, and the therapeutic effect is slow. The patient's compliance and satisfaction are average.

Method used

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  • Medical material for treating fungal keratitis and preparation method thereof
  • Medical material for treating fungal keratitis and preparation method thereof
  • Medical material for treating fungal keratitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The present embodiment provides a kind of medical material for the treatment of fungal keratitis, comprising the following steps:

[0044] S1: Preparation of Xanthomonas fermentation broth

[0045](1) Xanthomonas was inoculated in LB solid medium for activation, cultured at 28°C for 24-48 hours, and continuously transferred to obtain strains with good activity. In this step, Xanthomonas include Lysobacter zymogenes and Stenotrophomonas maltophilia, wherein Lysobacter zymogenes: strain number is ATCC29487 or Lysobacter zymogenes subspecies BNCC223409, BNCC203967, BNCC341391, LeYC36; Stenotrophomonas: the strain number is ATCC13637, ATCC17806, ATCC17808 or ATCC13270.

[0046] Preparation of LB medium: 10 g of peptone, 5 g of yeast extract, and 10 g of NaCl were successively dissolved in 1 L of distilled water, and the pH was 7.0-7.5. Raising the concentration of sodium chloride to 2% in the medium can increase the expression of dihydromaltophilin.

[0047] (2) Wash the...

Embodiment 2

[0064] By characterizing the physical and chemical properties of the medical composite material in Example 1, the results are as follows:

[0065] Depend on Figure 4 It can be seen that through the mechanical characterization of the biomechanical performance testing machine, the composite material has good operability and high tensile strength. The method for testing the tensile strength is: in a dry state, the sample is cut into a rectangular shape with a mold, and the width of the test part is 5mm. After the sample is soaked in deionized water for 30 minutes, it is measured by a Model 5567 biomechanical performance testing machine. The tensile strength of the sample, the tensile rate is 10mm / min. Each sample was tested 5 times.

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Abstract

The invention provides a medical material for treating fungal keratitis and a preparation method thereof. The preparation method comprises the following steps: S1, preparing xanthomonas fermentation liquor; S2, separating and purifying the fermentation liquor in the step S1, and extracting dihydromaltophilia; S3, respectively preparing a dihydromaltophilia solution A, a sulfobutyl ether beta cyclodextrin solution B and a collagen solution C; S4, dropwise adding the solution A into the solution B to obtain a mixed solution I, and freeze-drying the mixed solution I to obtain composite mixed powder; S5, dissolving the compound powder obtained in the step S4 into water to obtain a solution D, and then dropwise adding the collagen solution C to prepare a mixed solution II; and S6, pouring the mixed solution II in the step S5 into a mould, and drying in a constant-temperature and constant-humidity environment to obtain the medical material disclosed by the invention. The composite material provided by the invention can realize loading of thermostable antifungal factors, can be released on ocular surfaces in a use process, and can be used for treatment of fungal keratitis in the medical field.

Description

technical field [0001] The invention belongs to the field of medical materials, in particular to a medical material for treating fungal keratitis and a preparation method thereof. Background technique [0002] Fungal infection is a common infectious disease in ophthalmology and dermatology, and its incidence is high in developing countries and regions with warm or hot climates. Fusarium is the main pathogenic species of fungal infection in China, followed by Aspergillus and Candida. After the fungus adheres, it begins to reproduce in large numbers, releases toxins, and induces a series of immune responses. The body releases a large number of inflammatory factors, resulting in immune damage to the cornea or skin tissue. With the widespread clinical use of broad-spectrum antibiotics, glucocorticoids, and antiviral drugs, the incidence of fungal infections has gradually increased, and the condition has become more serious. At the same time, there are only a few drugs currentl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K31/407A61K47/42A61K47/40A61P31/10A61P27/02A61P29/00C12P39/00C12P13/02C12R1/01
CPCA61K31/407A61P31/10A61P27/02A61P29/00C12P39/00C12P13/02A61K9/7007A61K9/0048A61K47/42A61K47/40
Inventor 袁进赵晓霞赵轩钟菁左欣
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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