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Preparation method of selective medium of staphylococcus lentus

A staphylococcus, selective technology, applied in the field of medical testing, can solve the problems that Staphylococcus lentus cannot grow and can not be selectively cultivated Staphylococcus lentus, and achieve the effect of reliable detection and high isolation rate

Inactive Publication Date: 2013-11-13
姜晓丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this medium cannot selectively cultivate Staphylococcus lentus, and the reason is that Staphylococcus aureus grows faster than Staphylococcus lentus in the current selective medium, covering the medium quickly, and Staphylococcus lentus cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; add distilled water to 1000ml. Preparation method: take malt extract powder; egg protein liquid; sodium chloride; magnesium citrate; agar; After filtration, distilled water was used to make up to volume, sterilized twice at low temperature, each time for 45 min, and then packaged after sterilization.

Embodiment 2

[0014] Example 2, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; copper sulfate 5mg; distilled water added to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; , mixed and filtered, distilled water to volume, low-temperature sterilization twice, 45 min each time, after sterilization, aliquoted.

Embodiment 3

[0015] Example 3, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Hydroxyl-D-glucosamine 3.6 g; Potassium dihydrogen phosphate 0.2 g; L-cysteine 0.1 g; add distilled water to 1000ml. Preparation method: extract malt powder; egg white liquid; sodium chloride; magnesium citrate; agar; Dissolve in distilled water, mix and filter, distilled water to volume, sterilize twice at low temperature, 45 min each time, and pack after sterilization.

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PUM

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Abstract

The invention discloses a preparation method of a selective medium of staphylococcus lentus. The method is characterized in that a formula of a prepared 1000ml selective medium comprises 15g of malt extract powder, 150ml of an egg protein fluid, 5g of sodium chloride, 0.5g of magnesium citrate, 15g of agar, 8-12mg of coetsoidin A, 3.6g of N-acetyl-D-glucosamine, 0.2g of potassium dihydrogen phosphate, and the balance of distilled water for supplementing to 1000ml. The preparation method of the optimal formula comprises the following steps: dissolving malt extract powder, egg protein fluid, sodium chloride, magnesium citrate, agar, coetsoidin A, N-acetyl-D-glucosamine, potassium dihydrogen phosphate, L-cysteine and copper sulfate in a lily boiled liquid; uniformly mixing; fixing the volume; and sterilizing and sub-packaging. Compared with the prior art, the preparation method disclosed by the invention has the characteristics of reliable detection and high separation rate of staphylococcus lentus.

Description

technical field [0001] The present invention is filed on January 13, 2013, the application number is 2013100107502, and the name is a divisional case of the invention application of "A Selective Medium for Staphylococcus lentus and Its Preparation Method". The invention relates to the field of medical testing, in particular to a selective culture medium for Staphylococcus lentus and a preparation method thereof. Background technique [0002] Staphylococcus lentus belongs to the genus Staphylococcus. The bacterium was divided into an independent species of Staphylococcus by Schleifer K H et al. in 1983, that is, Staphylococcus lentus (S.lentus). Staphylococcus lentus was once considered non-pathogenic, but more and more data show that S.lentus is a pathogenic bacteria that cannot be ignored in humans or many animals, it can cause human diseases and play an important role in nosocomial infections, especially for Immunocompromised groups such as newborns, patients with seriou...

Claims

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Application Information

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IPC IPC(8): C12Q1/14
Inventor 候素君
Owner 姜晓丽
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