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A kind of staphylococcus lentus strain and use thereof

A staphylococcus, slow technology, applied in the field of microbiology, can solve problems such as animal husbandry and vegetation damage, destruction of aquatic ecological environment, etc., to achieve the effects of alleviating environmental hazards, environmental friendliness, and alleviating potassium deficiency

Active Publication Date: 2020-05-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water quality in the polluted surface water body will gradually acidify. When it is used for breeding or watering plants, it will cause irreversible damage to animal husbandry and vegetation, and destroy the aquatic ecological environment.

Method used

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  • A kind of staphylococcus lentus strain and use thereof
  • A kind of staphylococcus lentus strain and use thereof
  • A kind of staphylococcus lentus strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The isolation of staphylococcus lentus GZU-St01 comprises the following steps:

[0036] 1. Isolation and screening of functional strain GZU-St01

[0037] 1) Collection of samples

[0038] (1) The samples were collected from weathered coal gangue on a sulfur-containing coal gangue hill, and then the collected samples were put into plastic sample collection bags.

[0039] (2) Break up the collected samples.

[0040] (3) Sieve and collect 100-mesh samples.

[0041] (4) Put the samples in a refrigerator at 4°C for later use.

[0042] 2) Screening of functional strains

[0043] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water.

[0044] (2) Then shake for 30 minutes in a shaker at 170r / min and 37°C.

[0045] (3) For the control group, add 100mL sterile water to a 250mL Erlenmeyer flask.

[0046] (4) After half an hour, take the supernatant and gradually dilute to 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 .

[0...

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Abstract

The invention discloses a Staphylococcus lentus and its application. Its morphological characteristics: the appearance of the bacterial colony on LB medium: white, opaque, round, slightly raised, with neat edges, smooth and moist surface, shiny, and the size of the colony About 1mm. Microscopic morphology of the bacteria: Gram-positive cocci, arranged in clusters of grapes, without spores, flagella, and capsules. The invention can efficiently dissociate low-sulfur coal gangue to release potassium.

Description

technical field [0001] The invention belongs to the technical field of microbiology, specifically relates to a staphylococcus lentus, and also relates to the use of the bacterial strain in dissociating potassium in low-sulfur coal gangue. Background technique [0002] Coal is one of the main forms of energy at present. China's coal resources are abundant, widely distributed, and complete in variety. Coal plays a pivotal role in the development of the national economy. According to the level of sulfur content, coal can be divided into low-sulfur coal and high-sulfur coal. The sulfur content in coal varies greatly, with the highest being 8% and the lowest being 0.12%. After the exposed coal gangue is piled up, some harmful substances are dissolved after being washed by rainwater, and flow into the soil, surface water body or underground water body with the rainwater, causing serious pollution. Once the polluted groundwater is drunk by people, the health of the human body will...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/02C12R1/44
CPCC05F11/02C12N1/205C12R2001/44
Inventor 谢承卫高弦钟艳李春钢王柳兴李夏夏姜雄
Owner GUIZHOU UNIV
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