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Salt-tolerant staphylococcus lentus and application thereof in industrial NMP wastewater treatment

A staphylococcus, salt-resistant technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria and other directions, can solve the problems of difficult biodegradation of NMP industrial wastewater, common microorganisms cannot survive, and complex components, etc. The effect of good competitive advantage and application potential, low equipment requirements, and low processing cost

Pending Publication Date: 2020-09-22
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, NMP industrial wastewater often has complex components and contains more inorganic salt ions. Ordinary microorganisms cannot survive in such a high osmotic pressure environment, which brings difficulties to the biodegradation of NMP industrial wastewater.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: halotolerant staphylococcus slowus ( Staphylococcus lentus. ) Isolation and domestication of CCZU-X

[0020] The halotolerant staphylococcus lentus of the present invention ( Staphylococcus lentus. ) The separation and domestication process of CCZU-X is as follows:

[0021] 1) Samples, edible sea shrimp living in high-saline seawater in nature;

[0022] 2) Medium

[0023] Primary screening medium: LB medium. LB medium (g / L): peptone 10, yeast extract powder 5, sodium chloride 10, agar 20, pH 7.0.

[0024] Rescreening medium: LB selection medium with higher salt concentration. Selection medium (g / L): peptone 10, yeast extract powder 5, sodium chloride 10~200, pH 7.0.

[0025] 3) Primary screening, secondary screening and fermentation culture operation

[0026] Preliminary screening: put 5 g of edible sea shrimp living in high-salt seawater in nature into 100 mL enrichment medium, enrich and cultivate for 72 h at 37°C, 180 r / min, dilute with sterile w...

Embodiment 2

[0030] Embodiment 2: Halotolerant Staphylococcus lentus ( Staphylococcus lentus. ) Preparation of CCZU-X wet cells

[0031] The components of the activation medium were prepared according to the following concentrations: yeast extract powder 5 g / L, peptone 10 g / L, sodium chloride 10 g / L, pH natural, sterilized at 121°C for 20 minutes, cooled after sterilization, Insert the slant seeds (the halo-tolerant Staphylococcus lentum CCZU-X prepared in Example 1) and culture them for 1 day at 37°C with a shaker speed of 180 r / min as the seed solution for activation culture.

[0032] The components of the fermentation medium were prepared according to the following concentrations: sucrose 20 g / L, peptone 10 g / L, sodium chloride 10 g / L, pH 7.0, sterilized at 121°C for 20 minutes, cooled after sterilization, and inoculated according to The volume ratio of 10% was inoculated with the activated culture seed solution, and cultured for 1 day at a temperature of 37°C and a shaker speed of 180...

Embodiment 3

[0033] Embodiment 3: halotolerant staphylococcus slowus ( Staphylococcus lentus. ) Preparation of CCZU-X wet cells

[0034] The components of the activation medium were prepared according to the following concentrations: yeast extract powder 10 g / L, peptone 10 g / L, sodium chloride 10 g / L, pH natural, sterilized at 121°C for 20 minutes, cooled after sterilization, Insert the slant seeds (the halo-tolerant Staphylococcus lentum CCZU-X prepared in Example 1) and culture them for 1 day at 37°C with a shaker speed of 180 r / min as the seed solution for activation culture.

[0035]The components of the fermentation medium were prepared according to the following concentrations: yeast extract powder 5 g / L, peptone 10 g / L, sodium chloride 10 g / L, pH 7.0, sterilized at 121°C for 20 minutes, cooled after sterilization, Inoculate the activated culture seed solution with an inoculum volume ratio of 10%, and culture it for 1 day at a temperature of 37°C and a shaker speed of 180 r / min to o...

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Abstract

The invention discloses a strain of salt-tolerant staphylococcus lentus and application thereof in industrial NMP wastewater treatment, and belongs to the technical field of industrial wastewater treatment. The staphylococcus lentus CCZU-X is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.19016. The strain can be applied todegradation of NMP in industrial NMP salt-containing wastewater; in particular, suitable degradation culture conditions for NMP degradation are as follows: the temperature is 20-40 DEG C, the pH value is 5-9, and the concentration of NMP after treatment is greatly reduced. Compared with a traditional method, the strain and the application method thereof have the advantages that the treatment efficiency is high, the application range is wide, the operation is simple, the requirement on equipment is not high, the operation cost is obviously reduced, and the problem of low efficiency of treatingindustrial NMP salt-containing wastewater by a traditional activated sludge method can be solved.

Description

technical field [0001] The invention relates to the technical field of industrial wastewater treatment, in particular to a salt-tolerant Staphylococcus lentus and its application in industrial NMP wastewater treatment. Background technique [0002] Methylpyrrolidone (NMP) is used in natural gas recovery, lubricating oil refining, polymer reaction, etc. The residual NMP in wastewater will pollute the environment. [0003] At present, in the treatment of NMP wastewater, physical and chemical methods are generally used to separate NMP in wastewater. These methods all have problems such as high energy consumption, low efficiency and secondary pollution. Biological treatment is one of the most effective methods at present. The microbial degradability of NMP itself is good, and the content of NMP in wastewater can be reduced through microbial degradation. However, industrial NMP wastewater contains a high concentration of salt, which can inhibit the growth of common microorga...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34G01N30/02C02F101/38C02F101/34C12R1/44
CPCC12N1/20C02F3/34G01N30/02C02F2101/38C02F2101/34G01N2030/027C12R2001/44C12N1/205
Inventor 王利群章国瑞夏冉严生虎张跃
Owner CHANGZHOU UNIV
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