29 results about "Staphylococcus Phages" patented technology

The invention provides a kit for rapidly detecting MRSA and drug-resistant gene nucleic acid POCT of the MRSA based on a phage lyase mutant. The kit comprises nucleic acid extraction and purification and triple PCR rapid amplification detection reagents. The staphylococcus aureus bacteriophage lyase mutant nucleic acid release liquid containing the amino acid sequence as shown in SEQ ID NO: 1 is adopted, wall breaking can be rapidly conducted on staphylococcus aureus, and nucleic acid is released. The effect of the nucleic acid release liquid acting for 5 minutes is equivalent to or even better than that of the lysozyme acting for 60 minutes; according to the present invention, with the combination of the MRSA and drug-resistant gene triple PCR rapid amplification detection reagent, the total detection time is only 35 min, the time is saved by 55 min or more than 55 min compared with the lysozyme method, and the MRSA and drug-resistant gene nucleic acid POCT rapid detection can be achieved;

This invention discloses a broad-spectrum Staphylococcus aureus bacteriophage, SapYZUbeta, and its applications. This bacteriophage has been deposited at the China Center for Type Culture Collection (CCTCC) on May 16, 2022, with accession number CCTCC NO: M 2022629. Identification revealed that the full-length gene of this bacteriophage is 17,790 bp, and it does not contain tRNAs or genes associated with lysogenicity, virulence, or antibiotic resistance. This genetic background confirms the safety of this bacteriophage for use against foodborne pathogens and for bacteriophage therapy. Furthermore, the bacteriophage exhibits a high titer of 1.9 × 10⁻⁶. 9 The phage, with a concentration of PFU / mL, exhibits stable activity within a pH range of 3–12 and a temperature range of -80–60°C, and can be applied to various matrices. The phage provided by this invention can lyse multidrug-resistant wild-type strains isolated from different regions, including MRSA and MSSA, and is characterized by a broad lysis spectrum and high lysis efficiency.

Cosmetic composition comprising an endolysin derived from a Staphylococcus aureus phage and a mineral filler. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus phage and at least one mineral filler selected from hydrophilic fumed silicas and spherical porous silica particles. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic process for the care of keratinous materials, particularly skin, comprising at least one step of topical application of such a composition to said keratinous materials.

The present invention relates to a composition, in particular a cosmetic composition, comprising, in a physiologically acceptable medium, at least one endolysin derived from Staphylococcus aureus bacteriophage and at least one oil other than liquid paraffin. The invention also relates to the use of such a composition for the prevention and / or treatment of skin conditions associated with colonization of Staphylococcus aureus in a subject in need thereof, and in particular for the prevention and / or treatment of acne and / or eczema in a subject, and to a non-therapeutic cosmetic method for caring for keratin materials, in particular skin, it comprises at least one step of topically applying such a composition to the keratin material.

The invention relates to the technical field of microorganisms, in particular to a culture medium for staphylococcus aureus phage fermentation and a fermentation method. According to the culture medium for staphylococcus aureus bacteriophage fermentation, the problems that in large-scale culture of staphylococcus aureus bacteriophages, excessive accumulation of host bacteria biofilm precursor substances is caused by a high-nutrition environment is achieved through cooperation of whey protein hydrolysate, humic acid, chitosan and the like are solved, and the yield of the staphylococcus aureus bacteriophages is increased; the method solves the problems of bacteriophage infection efficiency reduction and amplification resistance, improves the bacteriophage titer, and shortens the fermentation period.

Cosmetic composition comprising an endolysin and hydrophobic silica aerogel particles. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus phage, and at least some hydrophobic silica aerogel particles. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic method for the care of keratinous materials, in particular skin, comprising at least one step of topical application of such a composition to said keratinous materials.

Cosmetic composition comprising an endolysin derived from a Staphylococcus aureus phage and an aromatic alcohol. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus phage and at least one aromatic alcohol of formula (I). It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic method for the care of keratinous materials, particularly skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

An anhydrous composition comprising an endolysin derived from a Staphylococcus aureus phage and mannitol. The present invention relates to an anhydrous composition comprising (i) at least one endolysin derived from a Staphylococcus aureus phage; and (ii) mannitol, the endolysin comprising a protein sequence having at least 80% sequence identity with an amino acid sequence selected from the group consisting of the reference amino acid sequences SEQ ID NO: 1 and SEQ ID NO: 2. It also relates to a composition comprising, in a physiologically acceptable medium, at least one anhydrous composition of the invention.It also covers the use of such a composition to prevent and / or treat a skin disorder related to Staphylococcus aureus colonization in an individual in need, and a non-therapeutic cosmetic process for the care of keratinous materials comprising at least one step of topical application of such a composition to said keratinous materials. Finally, it covers a process for stabilizing an endolysin derived from a Staphylococcus aureus phage. Figure for the abstract: None.

A cosmetic composition comprising an endolysin and trehalose. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus phage, and trehalose. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to Staphylococcus aureus colonization in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic method for the care of keratinous materials, in particular skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

L'Oréal Cosmetic Composition Comprising an Endolysin Derived from a Staphylococcus aureus Phage and 4-Hydroxyacetophenone. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus phage and 4-hydroxyacetophenone. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic method for the care of keratinous materials, particularly skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

Disclosed here are phage compositions for infecting and / or killing Staphylococcus spp.

The present invention relates to a composition, in particular a cosmetic composition, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus bacteriophage and at least one aromatic alcohol of formula (I). The invention also relates to the use of such a composition for the prevention and / or treatment of skin conditions associated with colonization of Staphylococcus aureus in a subject in need thereof, and in particular for the prevention and / or treatment of acne and / or eczema in a subject in need thereof, and to a non-therapeutic cosmetic process for caring for a keratin material, in particular skin, comprising at least one step of topically applying such a composition to said keratin material.

The present invention discloses a phage genome editing vector based on the CRISPR-Cas9 system and its editing method and application. red9 A plasmid containing the gene and sgRNA elements is constructed, and specific primers are designed for the editing site to obtain the pTarget plasmid; the donor DNA sequence is constructed into a vector to obtain the pEdit plasmid. After phage is infected with a host bacteriophage containing the pEdit plasmid, the pTarget plasmid is used to reverse screen for mutant phage, achieving efficient and rapid gene editing of the phage genome, including gene deletion mutations, single nucleotide substitution mutations, and insertion mutations. This method is simple to operate and has high gene editing efficiency. It has promoted the development of structural analysis and functional research of Staphylococcus aureus phage genomes and provided a new strategy for the modification of engineered phages, with broad application prospects and market value.

L'Oréal Cosmetic Composition Comprising an Endolysin Derived from a Staphylococcus aureus Phage and a Polyhydroxyalkane. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus phage and at least one polyhydroxyalkane of formula (I). It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic process for the care of keratinous materials, particularly of the skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

Cosmetic composition comprising an endolysin derived from a Staphylococcus aureus phage and an oil. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin derived from a Staphylococcus aureus phage and at least one oil distinct from a paraffin oil. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, in particular to prevent and / or treat acne and / or eczema in the individual, and a non-therapeutic cosmetic method for the care of keratinous materials, particularly of the skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

A cosmetic composition, particularly aqueous, comprising an endolysin and a pullulan. The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus phage, and at least one pullulan. It also relates to the use of such a composition to prevent and / or treat a skin disorder related to Staphylococcus aureus colonization in an individual in need, and in particular to prevent and / or treat acne and / or eczema in an individual in need, and a non-therapeutic cosmetic method for the care of keratinous materials, particularly skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

The present disclosure relates to novel enzymatically active domains from Staphylococcus phage proteins. The disclosure also relates to cell wall hydrolases comprising these enzymatically active domains and uses thereof in the treatment of infection with Staphylococcus sp.

The present invention relates to a composition, in particular a cosmetic composition, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus bacteriophage, and at least one nonionic surfactant comprising one or more carbohydrate residues. The invention also relates to the use of such a composition for the prevention and / or treatment of a skin disease associated with colonization of Staphylococcus aureus in a subject in need thereof, in particular for the prevention and / or treatment of acne and / or eczema in a subject in need thereof, and to a non-therapeutic cosmetic process for caring for a keratin material, in particular the skin, comprising the step of topically applying such a composition at least to said keratin material.

Cosmetic composition comprising an endolysin and a compound of formula (I) The present invention relates to a composition, particularly a cosmetic one, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus phage, and at least one compound of formula (I). It also relates to the use of such a composition to prevent and / or treat a skin disorder related to colonization by Staphylococcus aureus in an individual in need, in particular to prevent and / or treat acne and / or eczema in the individual, and a non-therapeutic cosmetic method for the care of keratinous materials, in particular skin, comprising at least one step of topical application of such a composition to said keratinous materials. Figure for abstract: None

An anhydrous composition comprising an endolysin derived from a Staphylococcus aureus bacteriophage and mannitol. The present invention relates to an anhydrous composition comprising (i) at least one endolysin derived from a Staphylococcus aureus bacteriophage; and (ii) mannitol, said endolysin comprising a protein sequence having at least 80% sequence identity to an amino acid sequence selected from the reference amino acid sequences SEQ ID NO: 1 and SEQ ID NO: 2. The invention also relates to a composition comprising, in a physiologically acceptable medium, at least one anhydrous composition of the invention. The invention also relates to the use of such a composition for the prevention and / or treatment of a skin condition associated with Staphylococcus aureus colonization in a subject in need thereof, and to a non-therapeutic cosmetic process for caring for a keratin material comprising at least one step of topically applying such a composition to the keratin material. The invention finally relates to a method for stabilizing endolysin derived from Staphylococcus aureus bacteriophage.

The invention belongs to the technical field of bioengineering, and provides a staphylococcus aureus bacteriophage and application thereof. The staphylococcus aureus bacteriophage provided by the invention is XHX-SA-1P, is preserved in the China Center for Type Culture Collection, has the preservation number of CCTCC NO: M2025494, and is classified and named as the staphylococcus aureus bacteriophage, the bacteriophage is obtained by separating and purifying an infected sample, the lysis spectrum is wide, the lysis efficiency is high, and the staphylococcus aureus bacteriophage has good environmental stability and multiplication capacity; the bacteriophage can be used for preparing an oral preparation, a freeze-drying preparation or a microcapsule preparation, can also be used as an active ingredient of a feed additive, and is used for preventing or treating diseases related to staphylococcus aureus infection of poultry and improving the biosafety level and the prevention and control effect in the breeding process.

Disclosed here are phage compositions for infecting and / or killing Staphylococcus spp.

The invention relates to the technical field of microorganisms, in particular to a culture medium for high-density culture of staphylococcus aureus bacteriophages and application of the culture medium. According to the present invention, the culture process is optimized, such that the titer of the efficient lytic MS9 phage (Staphylococcus aureus phage MS9) is improved to 10 < 10 > PFU / mL from the original 10 < 8 > PFU / mL, and the titer of the phage is significantly improved by two orders of magnitude. According to the technology, the production cost is greatly reduced, and the application of the staphylococcus aureus bacteriophage MS9 in prevention and control of staphylococcus is promoted.

The application provides a recombinant bacillus subtilis producing phage lytic enzyme LysJPP58 and a construction method and application thereof. The recombinant bacillus subtilis integrates the lytic enzyme LysJPP58 into the genome of a host bacterium bacillus subtilis, wherein the lytic enzyme LysJPP58 is derived from a staphylococcus aureus phage, and an amino acid sequence of the lytic enzyme LysJPP58 is shown as SEQ ID NO. 1. The recombinant bacillus subtilis can effectively secrete the LysJPP58 protein with lytic activity, and can significantly lyse staphylococcus aureus. The recombinant bacillus subtilis has the characteristics of safety, sustainability and industrial fermentation production, and provides a new technical approach for the development of animal health, food safety and medical antibacterial preparations.

The invention relates to the technical field of biology, in particular to a staphylococcus aureus bacteriophage SAP2, a preparation and application of the staphylococcus aureus bacteriophage SAP2. The staphylococcus aureus bacteriophage SAP2 is separated from a fecal sewage sample of poultry farms in Jiangxi Province. According to the specific embodiment of the invention, basic characterization and application effect evaluation are carried out on the phage through whole genome sequencing, electron microscope observation, biological characteristic experiments and the like, and the result shows that the staphylococcus aureus phage SAP2 not only is fast in replication and easy to amplify, but also has good stable activity; the bacteriophage has a wide lysis spectrum, can effectively inhibit the proliferation of staphylococcus aureus, and can be used as an antibody replacement product in practical application.

This invention belongs to the technical field of microbial antimicrobial agents, specifically disclosing a novel Staphylococcus aureus bacteriophage vB_SA_D5 and its applications. The bacteriophage vB_SA_D5 is deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), with accession number GDMCC No:68085-B1. The bacteriophage exhibits strong killing activity against Staphylococcus aureus and has an extremely broad host spectrum. It demonstrates good tolerance under conditions including pH 5-10, temperature 25℃-50℃, and chloroform. Whole-genome sequencing and bioinformatics analysis show that the bacteriophage does not carry any virulence or antibiotic resistance genes. Furthermore, this bacteriophage shows good control effects against the target bacteria in milk and fresh salmon, and can be safely applied to the biocontrol of Staphylococcus aureus.

The invention discloses a staphylococcus bacteriophage which is Staphyloccus pseudodintermedius Phage vBPTSHC01, the preservation number of the staphylococcus bacteriophage vBPTSHC01 is CCTCC (China Center For Type Culture Collection) NO: M 20252005, and application of the staphylococcus bacteriophage in preparation of a medicine for preventing and treating diseases infected by staphylococcus. The staphylococcus bacteriophage can split 29 swine staphylococcus aureus strains and 4 staphylococcus pseudointermedius strains, can continuously inhibit bacteria for a long time, is high in stability and good in safety, can be easily prepared into preparations and the like, and has very good killing, preventing and controlling effects on environments, foods and the like of staphylococcus.

The present invention relates to a composition, in particular a cosmetic composition, comprising, in a physiologically acceptable medium, at least one endolysin, in particular an endolysin derived from a Staphylococcus aureus bacteriophage, and at least one organic filler selected from the group consisting of unmodified starch, N-acylamino acids, salts thereof and mixtures thereof. The invention also relates to the use of such a composition for the prevention and / or treatment of skin diseases associated with colonization of Staphylococcus aureus in a subject in need thereof, in particular for the prevention and / or treatment of acne and / or eczema in a subject in need thereof, and to a non-therapeutic cosmetic method for caring for a keratin material, in particular the skin, comprising at least one step of topically applying such a composition to said keratin material.

The invention provides broad-spectrum salt ion tolerance staphylococcus aureus bacteriophage lyase and application, and belongs to the technical field of microorganisms. The lyase comprises an amino acid sequence shown as SEQ ID NO.2, or the lyase has at least 95% of homology with the amino acid sequence shown as SEQ ID NO.2 and has the same function as the amino acid sequence shown as SEQ ID NO.2. The staphylococcus aureus bacteriophage lyase provided by the invention has relatively good water solubility, salt ion and pH tolerance and temperature stability, is relatively wide in host spectrum, and can inhibit growth of methicillin-resistant and oxacillin-resistant staphylococcus aureus in chicken, clear biological membranes of the methicillin-resistant and oxacillin-resistant staphylococcus aureus, inhibit growth of the methicillin-resistant and oxacillin-resistant staphylococcus aureus, inhibit growth of the methicillin-resistant and oxacillin-resistant staphylococcus aureus and inhibit growth of the methicillin- no cytotoxicity exists.