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Preparing method for anti-microbial hemostatic chitosan and application of anti-microbial hemostatic chitosan

A technology of chitosan and polysaccharide, applied in the field of preparation of antibacterial hemostatic materials, can solve the problems of inability to absorb, easy escape of polyquaternary ammonium salt, harsh preparation conditions, etc., achieves simple and fast preparation method, enhanced hemostatic effect, excellent The effect of antibacterial properties

Active Publication Date: 2017-11-17
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an antibacterial and hemostatic material, its defect is that the antibacterial agent is composed of chitosan and silver, and the enhanced antibacterial effect is produced by silver, but silver is prone to excessive deposition, which affects the antibacterial effect
As an antibacterial and hemostatic material, its disadvantages are: the liquid absorption effect of chitosan fiber is poor, and it cannot absorb a large amount of exudate in the case of a large amount of bleeding, so it cannot quickly activate the coagulation mechanism, and the hemostatic effect is poor.
As an antibacterial and hemostatic material, its disadvantages are: the polyquaternium salt is compounded with chitosan through ethylenediaminetetraacetic acid, the stability is poor, and the polyquaternium salt is easy to escape, resulting in greater toxicity and affecting the antibacterial effect
However, the preparation conditions of this method are harsh, and specific preparation of carboxymethyl chitosan is required as a raw material. At the same time, the final product material prepared by selecting the existing quaternization reagent on the market is not satisfactory for medical hemostatic materials.

Method used

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  • Preparing method for anti-microbial hemostatic chitosan and application of anti-microbial hemostatic chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of antibacterial hemostatic chitosan, comprising the following steps:

[0041] 1) 1 g of epibromopropane was added dropwise to 10 mL of ethanol solution, and 5 g of tri-n-hexylamine was added dropwise to it, and the mixture was heated and stirred for 4 h at 40 °C.

[0042] 2) Add 1 g of chitosan (the degree of deacetylation is 80% and the molecular weight is 100,000) into 20 mL of sodium hydroxide solution (0.3%), dissolve 0.5 g of quaternary reagent A in 5 mL of ethanol, and then add quaternary The ammonium reagent solution was added dropwise to the chitosan solution, and the reaction was controlled at 45 °C for 24 hours. After the reaction was completed, it was washed three times with 70% ethanol solution, and then washed once with absolute ethanol, and then vacuum-dried to obtain Antibacterial hemostatic chitosan.

[0043] Utilize the above-mentioned antibacterial hemostatic chitosan to prepare the preparation method of antibacterial hemostatic ...

Embodiment 2

[0046] A preparation method of antibacterial hemostatic chitosan, comprising the following steps:

[0047] 1) 1 g of epichlorohydrin was added dropwise to 10 mL of ethanol solution, and then 2 g of N,N-dimethyl-n-octylamine was added dropwise to it, and the mixture was heated and stirred at 50°C for 2 hours. After the reaction was completed, the mixture was distilled under reduced pressure. The quaternization reagent B is obtained.

[0048] 2) Add 1 g of chitosan (with a deacetylation degree of 85% and a molecular weight of 200,000) into 10 mL of sodium hydroxide solution (0.3%), dissolve 0.35 g of quaternary ammonium reagent B in 3 mL of ethanol, and then dissolve the quaternary The ammonium reagent solution was added dropwise to the chitosan solution, and the reaction was controlled at 35 °C for 8 hours. After the reaction was completed, it was washed three times with 70% ethanol solution, and then washed once with absolute ethanol, and then vacuum-dried to obtain Antibacte...

Embodiment 3

[0052] A preparation method of antibacterial hemostatic chitosan, comprising the following steps:

[0053] 1) 1 g of epibromopropane was added dropwise to 10 mL of ethanol solution, and 5 g of tri-n-hexylamine was added dropwise to it, and the mixture was heated and stirred for 3 hours at 45°C.

[0054] 2) 1 g of epichlorohydrin was added dropwise to 10 mL of ethanol solution, and then 2 g of N,N-dimethyl-n-octylamine was added dropwise to it, and the mixture was heated and stirred at 40°C for 6 hours, and then distilled under reduced pressure after the reaction was completed. The quaternization reagent B is obtained.

[0055] 3) Add 1 g of chitosan (deacetylation degree of 85%, molecular weight of 300,000) into 10 mL of sodium hydroxide solution (0.3%), and dissolve 0.25 g of quaternary ammonium reagent A and 0.25 g of quaternary ammonium reagent B. In 6 mL of ethanol, the quaternary ammonium reagent solution was added dropwise to the chitosan solution, and the reaction was ...

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Abstract

The invention discloses a preparing method for anti-microbial hemostatic chitosan. The preparing method for the anti-microbial hemostatic chitosan comprises the steps that a cationic reagent solution is dropwise added in an epoxy reagent solution, the reaction temperature is controlled at 25-90 DEG C, reaction is conducted for 2-24h, pressure-reduction and distillation are conducted after the reaction is finished, and a quaternary ammonium reagent is obtained; the quaternary ammonium reagent is dissolved in a solvent, and a quaternary ammonium reagent solution is prepared; chitosan is added in an alkaline solution, then the quaternary ammonium reagent solution prepared in the step S1 is added and stirred for reaction, and the anti-microbial chitosan is obtained after washing and drying of raw products. The invention further discloses the application of the anti-microbialhemostatic chitosan. A chitosan anti-microbial hemostatic sponge is prepared, according to the standard of GB / T20944.3-2008, the detected bacteriostasis rate of staphylococcus aureus is greater than or equal to 98%, the bacteriostasis rate of escherichia coli is greater than or equal to 90%, and it is indicated that the prepared chitosan anti-microbial hemostatic sponge has the anti-microbial effect; the hemostatic effect of the prepared anti-microbial hemostatichemostatic sponge is excellent, and hemostatic time is less than or equal to 60 seconds; and the anti-microbial hemostatic sponge prepared by utilizing the chitosan has no obvious cytotoxicity, and biodegradation can be achieved.

Description

technical field [0001] The invention relates to a preparation method and application of an antibacterial hemostatic material, in particular to a preparation method and application of an antibacterial hemostatic chitosan. Background technique [0002] Bleeding and infection caused by trauma threaten the health and life of patients. How to prevent wound infection while hemostasis the wound is a medical requirement for biomedical materials, and it is also a hot spot in the current research on biomedical materials. [0003] Chitosan is obtained by deacetylation of chitin, which is widely present in nature, and its chemical name is polyglucosamine (1-4)-2-amino-B-D glucose. Chitosan is the only alkaline polysaccharide with cationic charges in nature, which can promote the aggregation of blood cells and hemoglobin, thereby promoting blood coagulation; chitosan can promote wound healing by mediating cell proliferation; chitosan is easy to dissolve in weak acid solvents, solubilizin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/0015A61L24/0036A61L24/0042A61L24/08A61L2300/232A61L2300/404A61L2300/418A61L2300/604A61L2400/04C08L5/08
Inventor 徐福建李杨罗旭胡杨苏洋
Owner BEIJING UNIV OF CHEM TECH
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