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Red-light carbon dot and nucleic acid-compound/nano-compound, preparation and application thereof

A nanocomposite and composite technology, applied in the fields of nanomaterials and biomedicine, can solve the problems of low photothermal conversion efficiency, poor biocompatibility, limited application, etc., and achieve high photothermal conversion efficiency, easy preparation, and strong absorption. and launch effects

Active Publication Date: 2021-03-19
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some photothermal conversion reagents have limited their application in tumor photothermal therapy due to factors such as complex synthesis, photobleaching, low photothermal conversion efficiency, and poor biocompatibility.

Method used

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  • Red-light carbon dot and nucleic acid-compound/nano-compound, preparation and application thereof
  • Red-light carbon dot and nucleic acid-compound/nano-compound, preparation and application thereof
  • Red-light carbon dot and nucleic acid-compound/nano-compound, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The iron phthalocyanine carbon dots prepared in this example can be used for fluorescence imaging and gene therapy. The preparation process includes:

[0036]Weigh 20mg of iron phthalocyanine into a beaker, add 40ml of DMF, sonicate for 30min, add 10mg of hydrogen peroxide and stir at room temperature for 5 minutes; add 10mg of 3rd generation dendritic arginine polypeptide lipid, and stir for 10min at room temperature;

[0037] Pour the above solution into a 100ml polytetrafluoroethylene-lined reaction kettle at a temperature of 180°C, react for 24 hours, and place it at room temperature to cool; add 160ml DMF to dilute 5 times, and then centrifuge at a speed of 5000rpm / min for 10 minutes , use a 0.22 μm filter head to remove large particles, and then use a dialysis bag with a molecular weight of MWCO=1000Da to dialyze in absolute ethanol for 24 hours, and change new absolute alcohol every six hours; after dialysis for 24 hours, vacuum distillation spin The solvent was ...

Embodiment 2

[0043] The amphiphilic iron phthalocyanine carbon dot nanocomposite prepared in this example can be used for fluorescence / photoacoustic / photothermal / magnetic resonance imaging and chemical kinetic / photothermal therapy for passive tumor targeting. The preparation process includes:

[0044] Weigh 20 mg of iron phthalocyanine into a beaker, add 40 ml of DMF, ultrasonicate for 30 minutes, add 10 mg of hydrogen peroxide and stir for 5 minutes at room temperature; add 5 mg of the second-generation dendritic arginine polypeptide lipid, and stir for 10 minutes at room temperature; pour the above solution into 100 ml reaction kettle lined with polytetrafluoroethylene, the temperature is 180 ° C, react for 24 hours, place it at room temperature to cool; add 160 ml DMF to dilute 5 times, then centrifuge at 5000 rpm / min for 10 minutes, filter with 0.22 μm First remove the large particles, and then use a dialysis bag with a molecular weight of MWCO=1000Da to dialyze in absolute ethanol for ...

Embodiment 3

[0050] In this example, water-soluble manganese phthalocyanine metal-doped carbon dots are prepared, which can be used for gene therapy; the preparation process includes:

[0051] Weigh 40 mg of manganese phthalocyanine compound into a beaker, add 60 mL of glycerol, sonicate for 30 minutes, add 30 mg of hydrogen peroxide, and stir for 5 minutes at room temperature; add 10 mg of polyethyleneimine with a molecular weight of 25 kD, and stir for 10 minutes at room temperature;

[0052] Pour the above solution into a 100ml polytetrafluoroethylene-lined reaction kettle at a temperature of 200°C, react for 24 hours, and place it at room temperature to cool; add 60mL glycerol to dilute twice, and centrifuge at 6000rpm / min for 10 minutes; Use a 0.22μm filter head to remove large particles, and then use a dialysis bag with a molecular weight of MWCO=1000Da to dialyze in absolute ethanol for 24 hours, and replace with new absolute alcohol every six hours; after dialysis for 24 hours, vacu...

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Abstract

The invention provides a red-light carbon dot, and belongs to the technical field of biological materials. The preparation method comprises the following steps: adding metal phthalocyanine into organic solvents, carrying out ultrasonic treatment, adding hydrogen peroxide, stirring the mixture at normal temperature, adding cationic polymers or cationic lipids to carry out a hydrothermal reaction, naturally cooling reaction products to room temperature after the reaction, and dilutting the cooled reaction products, centrifuging, filtering, dialyzing and drying to obtain water-soluble red-light carbon dots or fat-soluble red-light carbon dots. Two kinds of carbon dots, the water-soluble or fat-soluble carbon dots, can be obtained by adding different raw materials. The water-soluble carbon dots can be directly mixed with a gene drug to form a red-light carbon dot nucleic acid compound, and an amphiphilic red-light carbon dot nano-compound is obtained by adding amphiphilic macromolecules with good biocompatibility into the fat-soluble red-light carbon dots. The red-light carbon dot nucleic acid compound and the amphiphilic red-light carbon dot nano-compound can be applied to medical imaging and tumor treatment products, and the amphiphilic red-light carbon dot nano-compound is a multifunctional diagnosis and treatment integrated material with great clinical transformation potential.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedicine, and specifically relates to a red-light carbon dot and its nucleic acid complex / nano-composite, preparation and application. Background technique [0002] In 2004, scientists at the University of South Carolina in the United States discovered a new type of fluorescent carbon nanomaterial-carbon dots for the first time when studying the crude product of single-walled carbon nanotubes obtained by arc discharge. Carbon dots are a new type of zero-dimensional carbon material, whose particle size is usually less than 10nm. Carbon dots are widely used in biomedical imaging, nanomedicine, catalysis, and light-emitting devices due to their excellent water dispersibility, low toxicity, high photostability, and good biocompatibility. Especially in the field of nanomedicine, since most carbon dots do not have the inherent ability to generate heat or thermoacoustic signals under certai...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/22A61K49/12A61K41/00A61K33/44A61K48/00A61P35/00
CPCA61K33/44A61K41/0052A61K41/0057A61K48/0008A61K48/005A61K49/00A61K49/0002A61K49/0019A61K49/0054A61K49/126A61K49/22A61P35/00A61K2300/00
Inventor 金蓉蓉罗天荥聂宇
Owner SICHUAN UNIV
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