Composite membrane carrying bioactive factors PLA/PLGA/CS and preparation method thereof

A bioactive factor, composite membrane technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problems of difficult to control the direction and rate of drug release, unable to meet the time required for spinal cord repair, and irregular spinal cord injury. Achieve the effect of prolonging drug release time, helping nerve repair, and good drug release effect

Active Publication Date: 2020-06-02
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The above-mentioned scaffolds are made of biomaterials into catheters or blocks to guide nerve growth. Although they have good biocompatibility, they are difficult to apply clinically.
Because this kind of stent needs to be implanted after hemisectomy of the spinal cord, because the range of spinal cord injury in clinical patients is irregular and mostly incomplete, it is difficult to apply, and it is even more impossible to remove a segment of catheter placement
However, most drug-loaded composite membranes have a short drug release time, which cannot meet the time required for spinal cord repair, and its drug release direction and rate are also difficult to control.

Method used

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  • Composite membrane carrying bioactive factors PLA/PLGA/CS and preparation method thereof
  • Composite membrane carrying bioactive factors PLA/PLGA/CS and preparation method thereof
  • Composite membrane carrying bioactive factors PLA/PLGA/CS and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of PLA fiber membrane.

[0039] (1) dissolving PLA in hexafluoroisopropanol to form PLA solutions of 6wt%, 7wt% and 8wt% respectively;

[0040] (2) Put different concentrations of PLA solutions into plastic syringes with a capacity of 10ml, place the syringes horizontally on the syringe pump, cover a metal plate with a layer of aluminum foil as a receiver, and use a 20-gauge needle for the nozzle of the syringe. Electrospinning conditions: voltage 13-18kV, flow rate 0.08-0.1mm / min, receiving distance 12-15cm;

[0041] (3) The fiber membrane obtained in (2) is removed from the aluminum foil and freeze-dried to obtain a PLA fiber membrane.

Embodiment 2

[0042] Example 2: Preparation of PLGA microspheres.

[0043] (1) first dissolve PLGA in chloroform to be made into 5wt%, 6wt% and 7wt% PLGA solution, stir magnetically until completely dissolved;

[0044] (2) Electrospray PLGA solutions with different concentrations, put the electrospray solution into a plastic syringe with a capacity of 10ml, place the syringe horizontally on the syringe pump, and cover a metal plate with a layer of aluminum foil as a receiver. The nozzle of the syringe is a No. 22 needle. EFI conditions: Voltage 8-12kV, flow rate 0.06-0.08mm / min, receiving distance 12-15cm. Obtain PLGA microspheres.

Embodiment 3

[0045] Example 3: Preparation of drug-loaded microspheres with a chloroform solution concentration of PLGA of 6 wt % and water-to-oil ratios of 1:50, 1:100 and 1:200, respectively.

[0046] (1) PLGA is dissolved in chloroform, and magnetically stirred to completely dissolve, obtains the PLGA organic phase solution of 6wt%;

[0047] (2) An aqueous phase solution was prepared by dissolving NGF in deionized water. Then, the aqueous phase solution and the PLGA organic phase solution were mixed and sonicated (5 cycles, 10 seconds, 300W) to form emulsions with water-to-oil ratios of 1:50, 1:100, and 1:200, with a drug content of 0.5 μg / ml;

[0048] (3) Electrospray emulsions with different water-to-oil ratios, put the electrospray liquid into a plastic syringe with a capacity of 10ml, place the syringe horizontally on the syringe pump, and cover a metal plate with a layer of aluminum foil as a receiver , The syringe nozzle selects 22 gauge needles. EFI conditions: Voltage 8-12kV...

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Abstract

The invention discloses a composite membrane carrying bioactive factors PLA / PLGA / CS and a preparation method thereof. The mode of combining electrostatic spinning and electrostatic spraying is utilized, a PLA membrane is taken as a sealing layer, the diffusion of drugs can be prevented by utilizing the compactness of the PLA membrane, and mechanical support is provided; PLGA microspheres are takenas a sandwich layer to carry the drugs; and the large porosity and high biocompatibility of a CS membrane are utilized as a controlled-release layer to directly contact with damaged areas, and the purposes of neural restoration can be achieved by planning seed cells can be achieved, so that the PLA / PLGA / CS composite membrane with membrane / ball / membrane structures can be prepared. The composite membrane is stable in structure and has a certain mechanical strength, the tensile strength is 2.83+ / -0.31 Mpa, and the elongation at break is 53.25+ / -0.18%; the composite membrane can well carry the drugs and has good drug sustained-release effects, and the releasing of the drugs can achieve more than two months, so that the time required by the repairing of spinal cord injury can be met; and the composite membrane has no cytotoxicity and can successfully induce PC-12 cells to differentiate into neurons within 5 days, so that the composite membrane is good for neural restoration.

Description

technical field [0001] The invention belongs to the technical field of biocomposite membranes applied to the treatment of spinal cord injuries, and relates to a PLA / PLGA / CS composite membrane loaded with bioactive factors and a preparation method thereof. Background technique [0002] Spinal cord injury (spinal cord injury, SCI) is due to various reasons caused by various degrees of spinal cord or cauda equina injury in the spinal canal. Tissue engineering technology is currently the most promising method for the treatment of spinal cord injury. By selecting biomaterials with good biocompatibility, degradability, mechanical properties and low neurotoxicity, three-dimensional scaffolds are constructed and loaded to promote neuron growth or protect neurons. The drug provides a good microenvironment for the regeneration of the injured spinal cord to achieve the ideal effect of spinal cord repair. Chinese patent application CN102671238A discloses a microporous scaffold made of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/20A61L27/50A61L27/54A61L27/56
CPCA61L27/20A61L27/18A61L27/54A61L27/56A61L27/50A61L2300/414A61L2430/32C08L5/08C08L67/04
Inventor 宋晓丽许月顾军张欣钰李金芝
Owner YANGZHOU UNIV
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