Application of microcystin degrading enzyme in degradation of nodularin
A microcystin and degrading enzyme technology, which is applied in the field of water pollution control, can solve the problems of high requirements for reaction environment conditions and the application of restriction enzyme degradation technology, and achieve good degradation effect
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[0029] Example 1
[0030] 1. Material preparation
[0031] In this application, the method of "immobilizing microcystin degrading enzyme on graphene oxide material modified with L-cysteine" can refer to the patent application number "201410170050.4" and the title of the invention as "a kind of L -Cysteine-the method provided in "Method for preparing graphene oxide nanomaterials".
[0032] Specifically, in this application, the steps of immobilizing microcystin degrading enzyme on the graphene oxide material modified with L-cysteine are:
[0033] (1) Add graphene oxide to purified water and stir ultrasonically for 1 hour to obtain a relatively uniform graphene oxide suspension.
[0034] (2) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide sulfonate sodium salt to the above graphene oxide suspension to get mixed The carboxyl groups on the surface of the graphene oxide are activated under stirring conditions, and the activation reaction time is 1...
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