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Application of microcystin degrading enzyme in degradation of nodularin

A microcystin and degrading enzyme technology, which is applied in the field of water pollution control, can solve the problems of high requirements for reaction environment conditions and the application of restriction enzyme degradation technology, and achieve good degradation effect

Active Publication Date: 2019-06-18
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some studies have shown that free cyanotoxin degrading enzymes have high requirements on the reaction environment, which limits the application of enzymatic degradation technology

Method used

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  • Application of microcystin degrading enzyme in degradation of nodularin
  • Application of microcystin degrading enzyme in degradation of nodularin
  • Application of microcystin degrading enzyme in degradation of nodularin

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Embodiment 1

[0030] 1. Material preparation

[0031] In this application, the method part of "immobilizing microcystin-degrading enzymes on graphene oxide materials modified by L-cysteine" can refer to the patent application number "201410170050.4" and the invention name "a kind of L -The method provided in the preparation method of cysteine-graphene oxide nanomaterial".

[0032] Specifically, in the present application, the steps of immobilizing the microcystin-degrading enzyme on the graphene oxide material modified by L-cysteine ​​are:

[0033] (1) Add graphene oxide into purified water and stir ultrasonically for 1 hour to obtain a relatively uniform graphene oxide suspension.

[0034] (2) Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and sodium N-hydroxysuccinimide sulfonate to the above graphene oxide suspension to obtain a mixture The carboxyl group on the surface of graphene oxide is activated under stirring conditions, and the activation reaction time is 1h; gr...

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Abstract

The invention relates to the field of water pollution treatment, in particular to application of a microcystin degrading enzyme in degradation of nodularin. The microcystin degrading enzyme is immobilized to L-cysteine-modified graphene oxide material; the steps include (1) activating GO (graphene oxide) with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimidesodium salt, and modifying the activated GO with L-cysteine to obtain CysGO solution; (2) adding mlrA enzyme liquid into the CysGO solution, mixing well, carrying out shock reaction at 0 DEG C for 60min to obtain immobilized enzyme nano composite CysGO-mlrA solution, filtering the solution with a 0.22 mu m ultrafiltration membrane, washing the obtained solid with water, carrying out suction filtering, and drying to obtain the immobilized enzyme nano composite CysGO-mlrA. It is discovered by the inventor via experiments that the microcystin degrading enzyme can well degrade nodularin; CysGO-mlrA and NOD degraded products have no evident cytotoxicity to lymphocytes in zebrafish.

Description

technical field [0001] The invention relates to the field of water pollution control, in particular to the application of microcystin degrading enzymes in degrading nodular toxins. Background technique [0002] Cyanotoxins produced during freshwater cyanobacterial blooms threaten the health of aquatic ecosystems. Nodularin (NOD) is produced by Nodularin sp. Nodularia spumigena ) is a cyclic pentapeptide toxin, which has severe hepatotoxicity, genotoxicity, embryotoxicity and genotoxicity, and can inhibit the activity of eukaryotic phosphatase PP1 and PP2A, causing hyperphosphorylation of intracellular proteins, leading to cell apoptosis . At the same time, NOD can be enriched and transformed in organisms and the environment. Studies have found that NOD can eventually accumulate in flatfish, sturgeon, three sticklebacks, herring and salmon through the food chain and produce toxic effects, resulting in a large number of fish deaths. [0003] The chemical properties of alga...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N11/14C12N9/00C02F101/34C02F101/38
Inventor 吴湘吴昊叶金云张荣飞
Owner HUZHOU TEACHERS COLLEGE
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