Bacillus firums capable of efficiently degrading donkey hair

Abstract

The invention relates to bacillus firums R419 capable of efficiently degrading donkey hair. The strain can grow by taking untreated dry donkey hair as a unique carbon source and nitrogen source, efficiently degrades the donkey hair, generates compound amino acid, polypeptide and soluble protein, can specifically degrade the donkey hair, and has the donkey hair degradation rate of 91.8 percent and the remarkable degradation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Bacillus firmus R419 which secretes keratinase and efficiently degrades donkey hair and its application. Background technique [0002] The processing of donkey-hide gelatin produces a large amount of donkey hair waste, most of which is discarded, and the local accumulation and improper management of these materials can lead to serious environmental problems in addition to the loss of protein-rich materials. [0003] Donkey hair is mainly composed of hard keratin, which is a family of insoluble structural proteins and is the main component of mammalian hair, nails, hooves, horns, etc. Keratin waste constitutes a serious environmental pollutant; donkey hair is composed of more than 90% of keratin, which is mainly composed of amino acid residues, such as glycine, valine, serine and cysteine, and these amino acid residues and Other residues are cross-linked by disulfide bo...

AI Technical Summary

Problems solved by technology

[0006] Most keratinases have a wide range of substrates, which can not only hydrolyze soluble proteins such as gelatin, casein, bovine serum albumin, but also insoluble proteins such as hair, feathers, nails, phosphine, collagen and elastin; The different activities indicate that keratinase has substrate specificity and diversity. At present, most researches are on microorganisms for the purpose of degrading poultry feathers. There are few research reports on donkey hair degradation bacteria agents. Due to the complex structure of feathers, in addition to the main components In addition to keratin, it also contains about 15% of complex components called matrix, which have a greater impact on microbial degradation due to the large difference in structure and composition from feathers and donkey hair

[0007] Chinese patent document CN108004169A (application number: 201711364361.4) discloses Bacillus licheniformis ZL-1 and its application. The patent describes that Bacillus licheniformis ZL-1 can grow at a temperature of 20-60°C, and the growth temperature can reach The optimum reaction temperature of keratinase is 40-60°C, and it has high keratinase activity (keratinase activity is 52.14U / mL) at 45-55°C, and has a high degradation rate to feathers (degradation rate is 90.15%). Feathers and mushroom dregs have good growth in high-temperature rapid composting fermentation; but this invention does not relate to the degradation of donkey hair that the present invention is to solve

[0008] Chinese patent document CN110819572A (application number: 201911232925.8) discloses a kind of Bacillus pseudomycoides, which describes the inoculation of Bacillus pseudomycoides, after two days of fermentation, there are very few feathers left, and the products after feather degradation are measured, feather degradation liquid It contains 0.98% crude protein, 31.9g / L organic matter content, 4.02g / L free amino acid, 6.2g / L potassium and other components, and these components are all decomposed from feathers, which further proves that the strain has high efficiency. The effect of degrading feathers; but this invention does not relate to the degradation effect on donkey hair which the present invention is to solve

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