Bacillus firums capable of efficiently degrading donkey hair

A technology of Bacillus firmus and strains, applied in Bacillus firmus R419 and its application fields, can solve the problems of large difference in structure and composition, great influence of microbial degradation effect, complex feather structure, etc., to increase soil fertility and achieve remarkable degradation effect , good antioxidant effect

Active Publication Date: 2021-04-13
UNIV OF JINAN
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most keratinases have a wide range of substrates, which can not only hydrolyze soluble proteins such as gelatin, casein, bovine serum albumin, but also insoluble proteins such as hair, feathers, nails, phosphine, collagen and elastin; The different activities indicate that keratinase has substrate specificity and diversity. At present, most researches are on microorganisms for the purpose of degrading poultry feathers. There are few research reports on donkey hair degradation bacteria agents. Due to the complex structure of feathers, in addition to the main components In addition to keratin, it also contains about 15% of complex components called matrix, which have a greater impact on microbial degradation due to the large difference in structure and composition from feathers and donkey hair
[0007] Chinese patent document CN108004169A (application number: 201711364361.4) discloses Bacillus licheniformis ZL-1 and its application. The patent describes that Bacillus licheniformis ZL-1 can grow at a temperature of 20-60°C, and the growth temperature can reach The optimum reaction temperature o

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  • Bacillus firums capable of efficiently degrading donkey hair
  • Bacillus firums capable of efficiently degrading donkey hair
  • Bacillus firums capable of efficiently degrading donkey hair

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Example Embodiment

[0037]Experimental case

[0038]Screening of Strengthening Bacillus Bacillus Firums R419, including the following steps:

[0039](1) Select a strain of the corner-enzyme capacity to verify the degradation capability of the microorganism, first formulate the basic inorganic salt medium, which does not contain any carbon source and nitrogen source, so the microorganism can only pass Degradation of keratin in the hair to obtain carbon source, nitrogen source and energy;

[0040]The foundation inorganic salt medium is:

[0041]Mgso4· 7h2O 0.5g / L, K2HPO4 1g / l, NaCl 0.5g / L, FESO4· 7h2O 0.01g / L, margin water, pH 8.0-8.1;

[0042]The inorganic salt medium dispensed in each triangular bottle was 50 ml, sterilized.

[0043](2) Activation of strains: First, screen the laboratory from the bacteria in the bottom sludge of Jiazi Lake in Jinan University, was applied to LB (Yeast 5g / L, protein 胨 10g / L, sodium chloride 10g / L, agar 20g) / L, margin water, 121 ° C high pressure steam sterilization 20 min...

Example Embodiment

[0049]Example 1

[0050]The strain Bacillus Firums R419 is degraded with a sterile dry hair as a single carbon source, including the following steps:

[0051](1) First, the basic inorganic salt medium is first prepared, which does not contain any carbon sources and nitrogen sources, so the microbes can only obtain carbon sources, nitrogen sources and energy by degradation of keratin in the hair;

[0052]Basic inorganic salt medium ingredient: MGSO4· 7h2O 0.5g / L, K2HPO4 1g / l, NaCl 0.5g / L, FESO4· 7H2O 0.01g / l, margin water, pH 8.0-8.1;

[0053]The inorganic salt medium dispensed in each triangular bottle was 50 ml, sterilized.

[0054](2) The activation of strains: First, the bacteria Bacillus Firums R419 is applied to LB (yeast powder 5g / L, protein 胨 105 g / L, sodium chloride 10g / L, agar 20g / L, margin water, 121 EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc.

[0055](3) Preparation of seed culture fluid: The activated strain obtained by step (2) is inoculated into the liquid med...

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Abstract

The invention relates to bacillus firums R419 capable of efficiently degrading donkey hair. The strain can grow by taking untreated dry donkey hair as a unique carbon source and nitrogen source, efficiently degrades the donkey hair, generates compound amino acid, polypeptide and soluble protein, can specifically degrade the donkey hair, and has the donkey hair degradation rate of 91.8 percent and the remarkable degradation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Bacillus firmus R419 which secretes keratinase and efficiently degrades donkey hair and its application. Background technique [0002] The processing of donkey-hide gelatin produces a large amount of donkey hair waste, most of which is discarded, and the local accumulation and improper management of these materials can lead to serious environmental problems in addition to the loss of protein-rich materials. [0003] Donkey hair is mainly composed of hard keratin, which is a family of insoluble structural proteins and is the main component of mammalian hair, nails, hooves, horns, etc. Keratin waste constitutes a serious environmental pollutant; donkey hair is composed of more than 90% of keratin, which is mainly composed of amino acid residues, such as glycine, valine, serine and cysteine, and these amino acid residues and Other residues are cross-linked by disulfide bo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/54C05F11/08C05F1/00C05F17/20C12R1/07
CPCC12N1/20C12N9/54C05F11/08C05F1/005C05F17/20Y02W30/40Y02A40/20
Inventor 李强王芳宫晓范古鹏飞黄兆松樊祥宇
Owner UNIV OF JINAN
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