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Method for biologically degrading polycyclic aromatic hydrocarbon phenanthrene by using aspergillus niger fungi

A polycyclic aromatic hydrocarbon phenanthrene and biodegradation technology, applied in the field of bioremediation of organic pollutants, can solve the problems of affecting the biodegradation of PAHs, low degradation efficiency, and unsatisfactory degradation speed, achieving huge economic and social benefits and good degradation effect , Improve the effect of degradation speed and efficiency

Inactive Publication Date: 2017-05-31
王茹楠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many environmental factors may also affect the biodegradation of PAHs
Therefore, understanding the metabolism of microorganisms on PAHs plays an important role in the effective bioremediation of polluted environments. Phenanthrene is a representative of tricyclic polycyclic aromatic hydrocarbons, and biodegradation is the main way to remove phenanthrene in polluted environments. There have been many degradations of phenanthrene reported, but the currently known strains are not ideal for the degradation of phenanthrene, and the degradation efficiency is low

Method used

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  • Method for biologically degrading polycyclic aromatic hydrocarbon phenanthrene by using aspergillus niger fungi
  • Method for biologically degrading polycyclic aromatic hydrocarbon phenanthrene by using aspergillus niger fungi
  • Method for biologically degrading polycyclic aromatic hydrocarbon phenanthrene by using aspergillus niger fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the selection of phenanthrene initial concentration

[0020] 1. Preparation of solid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose and 20g of agar to the filtrate, dilute to 1000mL with water, and divide into test tubes Medium, sterilized at 112-125°C for 20 minutes to make a solid growth medium (slope);

[0021] 2. Preparation of liquid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose to the filtrate, dilute to 1000mL with water, and dispense into 250mL Erlenmeyer flasks medium, 40mL per bottle, sterilized at 112-125°C for 20min to make a liquid growth medium;

[0022] 3. Preparation of liquid nutrient-limited medium: chop 100g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double-layer gauze and collect the filtrate, add 10g o...

Embodiment 2

[0026] Example 2: Effect of pH on Degradation

[0027] 1. Preparation of solid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose and 20g of agar to the filtrate, dilute to 1000mL with water, and divide into test tubes Medium, sterilized at 112-125°C for 20 minutes to make a solid growth medium (slope);

[0028] 2. Preparation of liquid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose to the filtrate, dilute to 1000mL with water, and dispense into 250mL Erlenmeyer flasks medium, 40mL per bottle, sterilized at 112-125°C for 20min to make a liquid growth medium;

[0029] 3. Preparation of liquid nutrient-limited medium: chop 100g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double-layer gauze and collect the filtrate, add 10g of glucose to the filtrate, ...

Embodiment 3

[0033] Embodiment 3: Determination of optimal degradation time

[0034]1. Preparation of solid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose and 20g of agar to the filtrate, dilute to 1000mL with water, and divide into test tubes Medium, sterilized at 112-125°C for 20 minutes to make a solid growth medium (slope);

[0035] 2. Preparation of liquid growth medium: chop 200g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double gauze and collect the filtrate, add 20g of glucose to the filtrate, dilute to 1000mL with water, and dispense into 250mL Erlenmeyer flasks medium, 40mL per bottle, sterilized at 112-125°C for 20min to make a liquid growth medium;

[0036] 3. Preparation of liquid nutrient-limited medium: chop 100g of peeled potatoes, add 1000mL of water, boil for 30min, filter with double-layer gauze and collect the filtrate, add 10g of glucose t...

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Abstract

The invention discloses a method for biologically degrading polycyclic aromatic hydrocarbon phenanthrene by using aspergillus niger fungi. The method comprises the following steps: inoculating aspergillus niger fungi friendly provided by Chinese Academy of Sciences to a cant of a solid growth culture medium, culturing the aspergillus niger fungi in a culture box for 5-7 days at 28-30 DEG C to prepare cant strains; inoculating the cant strains into a liquid growth culture medium under aseptic condition, putting the liquid growth culture medium in a shaking bed for culturing for 24 hours under the condition with the temperature of 30 DEG C and the rate of 150r / min to prepare an aspergillus niger fungi degradation agent; adjusting different pH values in a liquid nutrition limitation medium, adding phenanthrenes with different concentrations, dissolving with assistance of trace of acetone, then sterilizing for 20 minutes at 112-125 DEG C, inoculating the aspergillus niger fungi degradation agent into the liquid nutrition limitation medium under the aseptic condition, culturing in the shaking bed under the condition with the temperature of 30 DEG C and the rate of 150r / min, and sampling and detecting after different times. Through the method, the degradation speed and the degradation efficiency are improved; an optimal degradation effect can be achieved under the conditions that the concentration of phenanthrene is 100 mug / mL, the pH is 7 and the culture time lasts for 6 days; the degradation rate reaches 85%; the method is great innovation for treating phenanthrene-containing wastewater and has great economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of organic pollutants, and in particular relates to a method for degrading polycyclic aromatic hydrocarbons, in particular to a method for biodegrading polycyclic aromatic hydrocarbon phenanthrene by Aspergillus niger. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of compounds that are commonly found in the environment and are composed of two or more benzene rings. Attracted attention, phenanthrene has a "bay" region (bay region) and a K region (kregion), making it a typical representative of polycyclic aromatic hydrocarbons. Therefore, it is of great significance to use phenanthrene as a substrate. Polycyclic aromatic hydrocarbons can Fully or partially degraded by a single microorganism or group of microorganisms, a variety of bacteria, fungi and algae with the ability to degrade PAHs have been isolated. The main reasons why PAHs can exist in the envir...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/14C12R1/685C02F101/32
Inventor 王茹楠
Owner 王茹楠
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