Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium, method and kit for rapidly culturing tumor organoids

A culture medium and organoid technology applied in the field of organoid culture to achieve the effect of improving the success rate and survival rate and shortening the required time

Active Publication Date: 2021-10-08
D1 MEDICAL TECH (SHANGHAI) CO LTD
View PDF11 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]Although a variety of tumor tissues can be successfully cultured into organoids in vitro using different methods and under different culture conditions, tumor organoid models are currently constructed in vitro and used to It takes 1-3 months to conduct drug or radiation testing, but the clinical demand window period is only 2-3 weeks

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium, method and kit for rapidly culturing tumor organoids
  • Culture medium, method and kit for rapidly culturing tumor organoids
  • Culture medium, method and kit for rapidly culturing tumor organoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0184] Example 1 Intestinal cancer organoid culture

[0185] 1. Reagent preparation

[0186] 1) Rapid proliferation medium for tumor organoids: composed of medium components Advanced DMEM / F12, 20% sterile egg white extract and specific additive factors; the composition of specific additive factors: HEPES, 10mM; GlutaMAX, 2mM; 1XB27, 1:100(v / v); 1X N2, 1:100(v / v); N-acetylcysteine, 1mM; R-spondin-1, 500ng / ml; Noggin, 100ng / ml; EGF, 50ng / ml ; Nicotinamide, 10 mM; gastrin I, 10 nM; A83-01, 500 nM; ProstaglandinE2, 10 nM; SB202190, 5 μM; FGF basic, 50 ng / ml; Mycin B mixture (100 U / mL penicillin, 100 μg / ml streptomycin, 250 ng / mL amphotericin B), 1X; Y-27632dihydrochloride, 10 μM. The added factor was 4% of the total volume of the medium.

[0187] 2) Sterile egg white extract

[0188] The preparation method of sterile egg white extract is as follows: take 1-3 days of fresh eggs, separate egg whites and pass through 100 μm, 70 μm, and 40 μm cell strainers respectively; and then ...

Embodiment 2

[0210] Example 2 Culture of intestinal cancer organoids

[0211] 1. Reagent preparation

[0212] 1) Rapid proliferation medium for tumor organoids: It consists of the medium components Advanced DMEM / F12, 20% sterile egg white extract and specific additive factors; the composition of specific additive factors: HEPES, 10mM; GlutaMAX, 2mM; 1XB27, 1:100(v / v); 1X N2, 1:100(v / v); N-acetylcysteine, 1mM; R-spondin-1, 500ng / ml; Noggin, 100ng / ml; EGF, 50ng / ml ; Nicotinamide, 10 mM; gastrin I, 10 nM; A83-01, 500 nM; ProstaglandinE2, 10 nM; SB202190, 5 μM; FGF basic, 50 ng / ml; Mycin B mixture (100 U / mL penicillin, 100 μg / ml streptomycin, 250 ng / mL amphotericin B), 1X; Y-27632dihydrochloride, 10 μM. The added factor was 4% of the total volume of the medium.

[0213] The composition of sterile egg white extract, tissue preservation solution, tissue digestion solution, and cell mass collection solution is the same as that in Example 1.

[0214] 2. Culture method of intestinal cancer orga...

Embodiment 3

[0229] Example 3 Culture of liver cancer organoids

[0230] 1. Reagent preparation

[0231] 1) Rapid proliferation medium for tumor organoids: It consists of the medium components Advanced DMEM / F12, 30% sterile egg white extract and specific additive factors; the composition of specific additive factors: HEPES, 10mM; GlutaMAX, 2mM; 1XB27, 1:100(v / v); 1X N2, 1:100(v / v); N-acetylcysteine, 1mM; R-spondin-1, 500ng / ml; EGF, 50ng / ml; gastrin I, 10nM; A83-01, 500nM; Forskolin, 10μM; FGF basic, 50ng / ml; FGF4, 50ng / ml; FGF10, 100ng / ml; Streptomycin, 250ng / mL Amphotericin B), IX; Y-27632dihydrochloride, 10 μM. The added factor was 4% of the total volume of the medium.

[0232] The composition of sterile egg white extract, tissue preservation solution, tissue digestion solution, and cell mass collection solution is the same as that in Example 1.

[0233] 2. Culture method of liver cancer organoids

[0234] The present embodiment provides a method for culturing liver cancer organoids...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of organoid culture, in particular to a culture medium, a method and a kit for rapidly culturing tumor organoids. According to the culture medium for culturing the tumor organoids provided by the invention, the time for culturing the tumor organoids can be greatly shortened. The kit for rapidly culturing the tumor organoid comprises a culture medium for culturing the tumor organoid, a tissue preservation solution, a tissue digestion solution and a cell mass collection solution, and when the kit is used for culturing the tumor organoid, the success rate and survival rate of culturing the tumor organoid can be increased, an organoid model can be rapidly established within 2-5 days, which can be applied to subsequent experimental tests.

Description

technical field [0001] The present invention relates to the field of organoid culture, in particular, the present invention relates to a culture medium, a method and a kit for rapidly culturing tumor organoids. Background technique [0002] At present, the incidence and mortality of cancer are increasing year by year. In 2020, there will be 4.57 million new cancer cases and 3 million cancer deaths in China. The huge economic and social burden caused by tumors is very heavy. [0003] Surgery, radiotherapy, chemotherapy, targeted therapy, immunotherapy and endocrine therapy are the main means of tumor treatment. About 70% of these patients require radiotherapy and chemotherapy, but due to individual differences in patients, tumor drug resistance or radiation resistance is an important factor restricting the successful treatment of tumors. At present, the overall effective rate of clinical tumor drugs is low (about 30%), and unnecessary treatment and ineffective treatment of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2509/00C12N2500/80
Inventor 富国祥李远闯林汉卿程春燕李俊强
Owner D1 MEDICAL TECH (SHANGHAI) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com