Polyploidy induction method for fiber linum usitatissimum under isolated culture condition

An in vitro culture and polyploid technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low induction rate and difficulty in obtaining tetraploid plants, and achieve high transplanting survival rate, The effect of polyploid plants with normal morphology and high ratio

Inactive Publication Date: 2011-07-13
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the present prior art, the method that induces fiber flax to produce polyploid mostly is to process the seed of fiber flax with colchicine, and the induction rate is low, and it is difficult to obtain a large amount of tetraploid plants in a short period of time, now In the prior art, the method of processing the shoot tip of fiber flax with colchicine to further improve the induction rate has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for inducing polyploidy in fiber flax under in vitro culture conditions, the method is carried out according to the following steps:

[0032] a. Select healthy and plump fiber flax Shuangya No. 7 seeds, disinfect the fiber flax Shuangya No. 7 seeds with 75% ethanol by volume for 5 minutes, pour out the ethanol, and then treat them with 0.1% mercuric chloride After 12 minutes, inoculate in B5 medium and cultivate for 6 days to obtain robust sterile vaccines;

[0033] b. Take robust aseptic seedlings, cut off the shoot apex and hypocotyl 1.3cm, put it in colchicine solution containing 0.05% by mass percentage and sterilized by 0.22μm filter membrane, at 25±2℃ The shaking speed is 100rmp, shaking for 12h, and then rinsed with sterile water for 3 times, cut off the shoot tip hypocotyl 0.3cm, and then transfer the shoot tip part to the shoot tip elongation medium for cultivation, and the shoot tip elongates Culture medium refers to the MSB solid medium added with 0...

Embodiment 2

[0043] A method for inducing polyploidy in fiber flax under in vitro culture conditions, the method is carried out according to the following steps:

[0044] a. Select the healthy and full fiber flax Shuangya No. 7 seeds, disinfect the fiber flax Shuangya No. 7 seeds with 75% ethanol by volume for 5 minutes, pour out the ethanol, and then treat them with 0.1% mercuric chloride by mass After 12 minutes, inoculate in B5 medium and cultivate for 7 days to obtain robust sterile vaccines;

[0045] b. Take robust aseptic seedlings, cut off the shoot tip with hypocotyl 1.4cm, put it in a colchicine solution containing 0.05% by mass percentage and sterilized by a 0.22μm filter membrane, at 25±2°C The shaking speed is 100rmp, shaking for 12h, and then rinsed with sterile water for 4 times, cut off the shoot tip hypocotyl 0.4cm, and then transferred to the shoot tip elongation medium for cultivation, the shoot tip elongation medium refers to Add the MSB solid culture medium of the gibb...

Embodiment 3

[0055] Fiber flax polyploid induction method under the in vitro culture condition, the method is carried out according to the following steps:

[0056] a. Select healthy and plump fiber flax Shuangya No. 7 seeds, disinfect the fiber flax Shuangya No. 7 seeds with 75% ethanol by volume for 5 minutes, pour out the ethanol, and then treat them with 0.1% mercuric chloride After 12 minutes, inoculate in B5 medium and cultivate for 8 days to obtain robust sterile vaccines;

[0057] b. Take robust sterile seedlings, cut off the shoot tip with hypocotyl 1.5cm, put it in a colchicine solution containing 0.2% by mass percentage and sterilized by 0.22μm filter membrane, at 25±2°C The shaking speed is 100rmp, shaking for 24h, and then rinsed with sterile water 5 times, cut off the shoot apex hypocotyl 0.5cm, and then transferred to the shoot apex elongation medium for cultivation, the shoot apex elongation medium refers to Add the MSB solid culture medium of the gibberellin of 1.0mg / L; ...

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Abstract

The invention relates to a polyploidy induction method for fiber linum usitatissimum under an isolated culture condition, wherein the method comprises the following steps of: selecting health and full fiber linum usitatissimum seeds; sterilizing the seeds and then culturing into seedling; processing the stem tip with colchicines to induce polyploid; transforming into a stem tip extending culture media for culturing; identifying the ploidy of the stem tip chromosome; culturing the stem section which is identified to be a tetraploid plant in a callus and bud induction culture medium to obtain lots of tetraploid sterile seedlings; and then transforming to an extending growing culture medium to grow; performing rooting culture in a rooting culture medium, and then transferring to a sterilized substrate for manging; and finally identifying the ploidy of chromosome of the root tip. The method can obtain higher inductivity than that of a method which processes the fiber linum usitatissimum seeds; the inductivity can be up to 25.5%; the pure tetraploid ratio is high; the polyploidy plant has normal shape, and high transplanting survival rate; the method can realize quick breeding under the isolated condition to obtain a large amount of tetraploid tissue-cultured seedlings in a short time.

Description

technical field [0001] The invention relates to a method for inducing polyploidy of plants under in vitro culture conditions, in particular to a method for inducing polyploidy of fiber flax under in vitro culture conditions. Background technique [0002] flax( Linum usitatissimum L., belonging to the family Linaceae, is an annual herb) is the earliest natural plant fiber used by humans, with a history of more than 10,000 years. Flax is a pure natural fiber, which is more and more valued by human beings because of its remarkable characteristics such as sweat absorption, good air permeability and harmlessness to human body. At present, the countries that plant flax for fiber in the world are mainly concentrated in the northern hemisphere within 45-65° north latitude, in the temperate and cold temperate regions of Europe and Asia. The main flax producing area in my country is Heilongjiang, whose geographical location and soil conditions are suitable for the growth of flax, ra...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王丽艳殷奎德郭永霞张兴梅荆瑞勇王北艳
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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