21 results about "Glandular Epithelial Cells" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to application of miRNA related to cow mastitis. The invention provides an application of miR-320b in preparation of a medicine for treating dairy cow mastitis, the nucleotide sequence of the miR-320b is shown as SEQ ID NO.1, and the miR-320b is used for diagnosing the process of resisting Escherichia coli, E. coli type dairy cow mastitis. The miR-320b expression quantity of the miR-320b in the dairy cow mammary epithelial cells or E. coli type mammary tissue induced by lipopolysaccharide (LPS) is detected, and when the expression quantity of the miR-320b is obviously reduced, the miR-320b is prompted to have an anti-inflammatory function in the dairy cow mastitis.

The invention discloses a method for regulating and controlling the growth of mammary epithelial cells of milk goats based on a KLC1-chi-miR-423-5p-PDGFAceRNA network, and belongs to the field of animal molecular biology and cell biology. Through combination of transcriptome sequencing and functional verification, it is revealed for the first time that KLC1 can be used as ceRNA, and targeted inhibition of chi-miR-423-5p on PDGFA mRNA is effectively relieved through competitive combination of chi-miR-423-5p, so that proliferation of mammary epithelial cells is promoted, and apoptosis is inhibited. The research results of the invention not only fill the blank of research on the regulation and control mechanism of the breast epithelial cell ceRNA network of the milk goat, but also provide a theoretical basis for breast development regulation and control, tissue repair and prevention and treatment of related diseases, and lay an important foundation for developing a potential molecular intervention strategy.

The invention relates to the technical field of cells, and particularly discloses an amino acid culture medium for promoting synthesis of casein of dairy cow mammary cells and a preparation method. In the culture medium, the addition amount of lysine is 0.74 mmol / L, the addition amount of threonine is 0.22 mmol / L, and the addition amount of phenylalanine is 0.28 mmol / L. Tests prove that the culture medium under the condition of the application can obviously improve the relative proliferation rate and casein expression quantity of dairy cow mammary gland cells compared with a culture medium under 4 times of plasma concentration (optimal amino acid supply mode) of dairy cow. The method for promoting casein synthesis comprises the following steps: culturing cow mammary epithelial cells to P3 generation; when the P3 generation cells grow to 75-85% of the adherent area in a 75 cm < 3 > culture flask, transferring the P3 generation cells into a culture medium without lysine, threonine and phenylalanine, and starving for 16 hours; when the cells are hungry, transferring the cells into the culture medium prepared by the invention to be continuously cultured for 24 hours; and after culture is finished, measuring the relative proliferation rate and casein expression quantity of the cells.

The present disclosure provides methods of manufacturing cell-laden scaffolds comprising lipid-producing cells comprising confining lipid-producing precursor cells in a three-dimensional (3D) environment and culturing the lipid- producing precursor cells confined in the 3D environment in a food-grade differentiation medium to obtain the cell-laden scaffold comprising lipid-producing cells. In one embodiment, the 3D environment is formed by a mixture comprising lipid-producing precursor cells and a food-grade polymericsolution, wherein the food-grade polymeric solution comprises one or more selected from the group consisting of alginate, gelatin, pectin, agarose, carrageenan, gellan gum, konjac glucomannan, cellulose, etc. and combinations thereof, the lipid-producing precursor cells are selected from the group consisting of adipose-derived stem cells, preadipocytes mesenchymal stem cells, embryonic stem cells, induced- pluripotent stem cells and combinations thereof, the lipid-producing cells are selected from adipocytes, hepatocytes, sebocytes, mammary epithelia cells, keratinocytes and combinations thereof, and the food-grade differentiation medium comprises a fatty acid.

The invention discloses application of MISP as a breast cancer diagnosis / prognosis marker and a treatment target. The in-depth study on the expression of MISP in breast cancer cells and the influence on malignant biological behaviors such as breast cancer proliferation shows that compared with normal breast epithelial cells, the MISP is highly expressed in the breast cancer cells, the expression of the MISP is highly related to poor clinical prognosis of breast cancer patients, and the proliferation of the breast cancer cells can be inhibited by reducing the expression of the MISP. A new biomarker is provided for diagnosis and prognosis of the breast cancer, a new target spot is provided for treatment of the breast cancer, and important scientific significance is achieved in the aspects of subsequent drug research and development, clinical treatment and the like.

The present disclosure provides thymic cells, thymic organoids, and animal models comprising such thymic cells and thymic organoids. The present disclosure also provides methods for inducing, in vitro and in vivo, differentiation of pluripotent stem cells into definitive endoderm (DE), anterior and anterior intestinal endoderm (AFE), ventral pharyngeal endoderm (VPE), thymic epithelial progenitor (TEP) cells, thymic epithelial cells (TEC), and thymic organoid.

The application discloses a method for improving the passaging times of a mammary epithelial cell line and application thereof, and belongs to the technical field of mammary epithelial cell lines. The application first finds that the exogenous fragment HS4-SAR fragment in the attachment area of the insulator-scaffold is connected with a p-Ef1a-SV40LT-Puro vector or a pEF1a-eGFP vector, and the transfection of commercial immortalized cell lines and primary cell lines can significantly improve the passaging times of the mammary epithelial cell lines, the cell morphology is still normal after 120 generations, the malignant transformation does not occur, the gene expression level does not decrease, and the immortalization is realized; the established goat mammary epithelial cell line still has the ability of synthesizing and secreting proteins; the method for improving the passaging times of the mammary epithelial cell line is simple and easy to operate, has a significant effect on the immortalized cell lines and the primary cell lines, and is expected to improve the stable passaging times of various mammary epithelial cell lines, and reduce the cell cost of related research.

The application relates to the technical field of biology, and discloses a method for inducing a cell line of immortalized musk deer scent glands to synthesize dihydrotestosterone, wherein the cell line is obtained by transfecting a primary musk deer scent gland epithelial cell with a lentivirus carrying an SV40 Large T antigen and performing resistance screening; the cell line is in an epithelial cell form and stably expresses keratin 18, cholesterol side chain cleavage enzyme, 17 alpha-hydroxylase and 3 beta-hydroxysteroid dehydrogenase. Through lentivirus-mediated SV40 Large T antigen transfection, the primary musk deer scent gland epithelial cell breaks through the limit of replicative senescence and obtains unlimited proliferation capacity, effectively overcomes the defects that the primary cell is prone to fibrosis and rapidly loses metabolic function during in-vitro culture, provides a standardized cell model with a clear genetic background and persistent metabolic activity for biological manufacturing of dihydrotestosterone, and relieves the dependence on endangered animal live resources.

The invention relates to the technical field of molecular biology, in particular to an MUC15 gene segment, expression vector construction of the MUC15 gene segment and an expression vector transfection method of the MUC15 gene segment, and the nucleotide sequence of the MUC15 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1. The MUC15 gene segment is obtained by amplifying a primer pair, and the primer pair is used for amplifying the buffalo mammary epithelial cell triglyceride and fatty acid synthesis. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGTTGACTCAGCCAAAATTCTG-3', F: 5 '- And R: 5 '-AATTCGAAGCTTGAGCTCGAGCTTACACGAAGTGGAGG-3'. Compared with the prior art, the buffalo mammary epithelial cell MUC15 gene fragment influencing the synthesis of triglyceride and fatty acid in the buffalo mammary epithelial cell is obtained, and a pEGFP-C1-MUC15 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.

A method of producing mammalian breast milk exosomes, such as human breast milk exosomes, comprising generating lactating cells derived from mammalian breast epithelial cells, such as human breast epithelial cells, expressing mammalian milk-like products, such as human milk-like products, from the lactating cells, and purifying the exosomes from the mammalian milk-like products.

The present invention provides a thymic epithelial cell production method, the method including a step for culturing thymic epithelial progenitor cells for a long period of time. The present invention also provides a transplant therapeutic agent that includes thymic epithelial cells produced by the production method according to the present invention. The present invention further provides a T cell production method, the method including a feature of causing thymic epithelial cells produced by the production method according to the present invention to contact hematopoietic stem cells or any cells in the process of differentiation from hematopoietic stem cells to T cells.

The invention discloses an application of GW9508 in preparation of a product for improving thymus function degeneration caused by aging, in-vivo and in-vitro experiments prove that the GW9508 can improve the aging state of thymus epithelial cells, promote the activity of the aging thymus epithelial cells, increase the thymus index of an aging mouse, increase the number of the thymus epithelial cells and subpopulation cells of the thymus epithelial cells and improve the thymus function degeneration caused by aging. The GW9508 has the advantages that the GW9508 can be used for improving the thymus function of an aged mouse, enhancing the thymus cell maturation tending capacity of the GW9508, increasing the number of thymus cells and subgroups of the thymus cells, and having an outcome effect on thymus of the aged mouse, so that the GW9508 can improve the thymus function of the aged mouse by improving the aging of the thymus, and the GW9508 can be used for preparing a product for improving thymus function degradation caused by aging.

The invention discloses an immortalized egg shell gland epithelial cell line and application thereof. The invention belongs to the technical field of biology, and particularly relates to an immortalized egg shell gland epithelial cell line and application thereof. The construction method of the immortalized egg shell gland epithelial cell line comprises the following steps: 1) obtaining SV40 overexpression lentivirus; 2) transfecting the lentivirus obtained in the step 1) to chicken primary eggshell gland epithelial cells; amplifying the transfected cells for 3-4 generations, and screening puromycin to obtain successfully transfected eggshell gland epithelial cells; and (3) carrying out subculture on the successfully transfected eggshell gland epithelial cells obtained in the step (2) to obtain the stably subcultured immortalized eggshell gland epithelial cells. The immortalized egg shell gland epithelial cell provided by the invention can provide a reliable cell model for researching related functions of poultry egg shell glands, and has an important value for developing a novel feed additive capable of improving chicken genital tract health and egg shell quality.

The invention belongs to the technical field of biology, and particularly relates to application of baicalin to preparation of a product for promoting milk fat synthesis of cow mammary epithelial cells. Researches show that baicalin can promote expression of milk fat synthesis related genes in bMECs and formation of lipid droplets, then milk fat synthesis is promoted, and the influence of oxidative stress on milk fat synthesis of bMECs is relieved. In addition, baicalin can promote the level of key protein in milk fat synthesis in bMECs.

This disclosure provides thymocytes, thymic organoids, and animal models comprising such thymocytes and thymic organoids. It also provides methods for inducing the differentiation of pluripotent stem cells into endoderm (DE), anterior foregut endoderm (AFE), ventral pharyngeal endoderm (VPE), thymic epithelial precursor (TEP) cells, thymic epithelial cells (TEC), and thymic organoids in vitro and in vivo.

The invention relates to the technical field of molecular biology, in particular to an ACSL3 gene segment, an expression vector construction method thereof and an expression vector transfection method thereof, the nucleotide sequence of the ACSL3 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1, the ACSL3 gene segment is obtained by amplifying a primer pair, and the primer pair has a nucleotide sequence shown as SEQ No.2. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGAATAACCAGGGTCTACAA-3', F: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 ' And R: 5 '-AATTCGAAGCTTGAGCTATGAGCTATTTTCTTCCATATTCGCTCA-3'. Compared with the prior art, the ACSL3 gene fragment influencing the synthesis of the buffalo mammary epithelial cell triglyceride and fatty acid is obtained, and a pEGFP-C1-ACSL3 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of the triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.

The invention discloses a chromene compound for resisting breast cancer as well as a preparation method, application and a preparation of the chromene compound. The chromene compound has a structural formula shown in a chemical formula 1. The application is the application of the chromene compound in preparation of anti-breast cancer drugs. The preparation comprises the chromene compound and pharmaceutically acceptable auxiliary materials. The chromene compound provided by the invention has excellent inhibitory activity on breast cancer cells and low toxicity on normal mammary epithelial cells, and provides a new choice for preparing anti-breast cancer preparations.

This invention discloses a novel compound and its application in the preparation of drugs for treating triple-negative breast cancer. The invention provides a compound with a novel structure; activity studies show that this compound has excellent therapeutic effects on triple-negative breast cancer, not only inhibiting the growth and migration of triple-negative breast cancer cells but also inducing apoptosis in triple-negative breast cancer cells, while having no significant inhibitory effect on normal breast epithelial cells, and exhibiting a safety profile superior to cisplatin. This invention also provides a simple and feasible method for preparing this compound.

The invention provides isolated human breast milk exosomes, and compositions thereof. The present invention also provides in vitro methods for producing said exosomes comprising generating lactocytes derived from mammalian mammary epithelial cells, for example human mammary epithelial cells, expressing the mammalian milk like product, for example the human milk like product from lactocytes, and purifying the exosomes from the mammalian milk like product. The invention also provides therapeutic uses of the isolated human breast milk exosomes, and compositions thereof.

The application relates to application of a probiotic preparation in improving dairy cow mammary gland health, and belongs to the technical field of probiotic preparation application. The application finds that a microbial preparation prepared from Akkermansia muciniphila is helpful to maintaining the dairy cow mammary gland health, thereby improving the milk yield and milk quality of the dairy cow, by promoting the proliferation of the dairy cow mammary gland epithelial cells, inhibiting the apoptosis of the dairy cow mammary gland epithelial cells (alleviating mammary gland inflammation and promoting the expression of milk secretion related genes). The preparation is safer, more efficient and pollution-free, is non-toxic and harmless, does not cause negative influence on the health of the dairy cow, and is easy to obtain, easy to culture in a large amount, simple to operate, easy to obtain raw materials and simple in method, so that the cost of manpower and material resources can be reduced.

To provide a method for making a bioengineered thymus organoid.SOLUTION: A method has the steps of: obtaining a cell population having human thymic epithelial progenitor cells or human thymic epithelial cells or both; combining the cell population with human hematopoietic stem cells in a defined ratio to form a combination; seeding the combination into an extracellular matrix of a decellularized thymus scaffold to generate a thymus construct; and culturing the thymus construct under conditions permitting cellular attachment onto the extracellular matrix thereby making the bioengineered thymus organoid.SELECTED DRAWING: None