Frozen stock solution for storing mammary epithelial cells of milk goat and freeze-saving method

A technology of mammary epithelial cells and cryopreservation method, which is applied in the field of cryopreservation solution for dairy goat mammary epithelial cells, which can solve the problems of single protective component, affecting cell survival, and long interval time, so as to shorten the proliferation cycle, simplify the process, Morphologically normal effect

Inactive Publication Date: 2015-04-08
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above method, the protective component in the cell cryopreservation solution is single, there are many

Method used

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  • Frozen stock solution for storing mammary epithelial cells of milk goat and freeze-saving method
  • Frozen stock solution for storing mammary epithelial cells of milk goat and freeze-saving method
  • Frozen stock solution for storing mammary epithelial cells of milk goat and freeze-saving method

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Embodiment 1

[0030] A cryopreservation solution for preserving dairy goat mammary gland epithelial cells, diluted by 1mL of insulin-transferrin-sodium selenium into 100mL of basic cryopreservation solution, said basic cryopreservation solution is formed by mixing the following components:

[0031] DMEM / F12 culture medium 30mL;

[0032] Fetal bovine serum 60mL;

[0033] Dimethyl sulfoxide 10mL.

Embodiment 2

[0035] A cryopreservation solution for preserving dairy goat mammary gland epithelial cells, diluted by 1.5mL of insulin-transferrin-sodium selenium into 100mL of basic cryopreservation solution, said basic cryopreservation solution is formed by mixing the following components:

[0036] DMEM / F12 culture medium 40mL;

[0037] Fetal bovine serum 50mL;

[0038] Dimethyl sulfoxide 10mL.

Embodiment 3

[0040] A cryopreservation solution for preserving dairy goat mammary gland epithelial cells, diluted by 2mL of insulin-transferrin-sodium selenium into 100mL of basic cryopreservation solution, said basic cryopreservation solution is formed by mixing the following components:

[0041] DMEM / F12 culture medium 50mL;

[0042] Fetal bovine serum 40mL;

[0043] Dimethyl sulfoxide 10mL.

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Abstract

The invention discloses a frozen stock solution for storing mammary epithelial cells of a milk goat. The frozen stock solution is prepared by mixing a basic frozen stock solution and insulin-transferrin-sodium selenate in a volume ratio of 100 to (1-2). The basic frozen stock solution is prepared by mixing 10% by volume percent of dimethyl sulfoxide, 30-50% by volume percent of a DMEM/F12 culture medium and 40-60% by volume percent of fetal calf serum. The freeze-saving frozen stock solution disclosed by the invention is few in step, simplified in process and low in cost, the resuscitated mammary epithelial cells are strong in anti-apoptosis performance, oxidization resistance and multiplication capacity, the resuscitation rate of the mammary epithelial cells can be increased, and the vitality of the resuscitated cells reaches up to above 95%, so that the cell growth quality is ensured, the proliferation cycle of cell resuscitation is shortened and the mammary epithelial cells of the milk goat can be saved for a long time.

Description

Technical field: [0001] The invention relates to the technical fields of tissue and cell engineering and cell biology, in particular to a cryopreservation solution and a cryopreservation method for preserving dairy goat mammary gland epithelial cells. Background technique [0002] Dairy goat mammary epithelial cells can not only be used as a model for studying milk protein gene expression and apoptosis, but also as a detection system for mammary gland-specific expression vectors. At present, although the method of in vitro isolation and culture of dairy goat mammary epithelial cells has been basically established, there is no recognized immortalized dairy goat mammary epithelial cell line. Dairy goat mammary epithelial cells are primary cultured cells, which need to be purified and subcultured to obtain functional mammary epithelial cells, which not only takes a long period of culture, but also causes a lot of waste of reagents and materials; As the number of cell cultures ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 曹艳红关志惠黄俊翔周恒莫柳忠韦明松
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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