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Process for preparing triticeae chromosome specimen

A technique for making specimens and chromosomes, applied in the field of cytogenetics and cell biology, can solve the problem of difficulty in obtaining chromosome specimens, and achieve the effect of simple operation

Inactive Publication Date: 2006-10-11
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of chromosome specimens requires certain experience
Therefore, for beginners and sometimes even for experienced persons, it is often difficult to obtain sufficient chromosome samples of metaphase, thus spending a lot of valuable time and energy in research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The chromosome specimen of embodiment 1 common wheat is made

[0029] first day

[0030] Soak the seeds at 8:00. First, soak the seeds in warm water at 30-32°C for 2-5 minutes, wash them for 3-5 times, rub them with your hands or brush to remove the floating soil on the surface of the seeds; in the thermostat. Change warm water at 26±1°C 1-2 times in the middle.

[0031] 20:00 hair roots. Spread the filter paper in a clean petri dish, add a small amount of tap water, the filter paper is wet and tilt the petri dish to see a small amount of water, it is advisable to have 0.8-1ml of rich water in a petri dish with a diameter of 10cm; put the seeds on the wet filter paper , along the Petri dish for a week, with the buds facing the center of the dish. 30 seeds were placed in each petri dish with a diameter of 10 cm. Germinate in the dark in an incubator at 26±1°C.

[0032] the next day

[0033] 8:00 or 20:00 for 0 ~ 4 ℃ treatment. Pick seeds with a root length of ab...

Embodiment 2

[0041] Embodiment 2 Chromosome specimen making of shelled seeds such as barley, oat, wheatgrass and wheatgrass

[0042] first day

[0043] Rinse with running water in advance to remove floating soil and empty shells on the surface of the seeds.

[0044] Soak the seeds at 8:00. Seeds were soaked in warm water at 30-32°C and placed in a constant temperature box at 26±1°C. In the middle, change warm water at 26±1°C for 3 to 4 times.

[0045] The following process is the same as in Example 1.

Embodiment 3

[0046]Embodiment 3 Chromosomal samples of rye, highland barley (naked barley), emmer wheat, emmer wheat, triticale and naked oats (naked oats) etc.

[0047] Whole flow process is with embodiment 1.

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PUM

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Abstract

The invention belongs to the field of cytogenetics and cell biology, and relates to a method for preparing a chromosome sample. Aiming at the problem that it is often difficult for operators to obtain enough metaphase chromosome specimens in the production of chromosome specimens, the present invention discloses a method for preparing wheat chromosome specimens, which includes seed soaking, germination, low temperature treatment, rewarming growth, pre- Processing, fixation and other steps. The method of the invention is simple to operate, and even a beginner can obtain enough metaphase cleavage phase by using the method.

Description

technical field [0001] The present invention belongs to the field of cytogenetics and cell biology. Background technique [0002] Wheat is an important food crop for humans. Chromosome research is indispensable in the study of the origin and evolution of wheat, cross-breeding, genetic rules and identification of exogenous chromosomes. The work on wheat chromosomes has also made great contributions to the theory of cytogenetics and breeding practice, such as the establishment of monosomy, terminal body, missing body, tetrasomy, various additional lines, haploid, polyploid, etc. research and application. In the genome and post-genome era, the study of chromosomes still plays an important role. Inheritance in eukaryotes is based on chromosomes. In genome research, the drawing of genetic maps and physical maps, and the chromosome location of genes are all related to chromosome research. In recent years, synteny research is of great significance for rev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28A01N3/00G09B23/38C12N11/00C12N15/00
Inventor 郭骁才
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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