Method for rapidly preparing metaphase chromosomes of marine valuable and rare fishes or shellfishes

A chromosome and marine technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of time-consuming, heavy cost, and execution of test subjects, etc., and achieve easy operation, good dispersion, simple and fast method Effect

Active Publication Date: 2016-12-21
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, many marine fish and shellfish are rare species. If the traditional method is used to prepare chromosomes, it will not only take a long time, but also have to kill the test subjects, which is extremely expensive

Method used

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  • Method for rapidly preparing metaphase chromosomes of marine valuable and rare fishes or shellfishes
  • Method for rapidly preparing metaphase chromosomes of marine valuable and rare fishes or shellfishes

Examples

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Embodiment 1

[0022] In April 2010, the 50L water tank in the laboratory was used to temporarily raise a 1-year-old female fish, 23cm in length, for about 30 days. The corrugated lip fish is a high-level ornamental fish and a high-level edible fish. Due to overfishing, in just a few years, the World Wide Fund for Nature has listed it as one of the 10 endangered biological species (Encyclopedia Knowledge, Ten Great List of Threatened Species, 2005, (6): 2,67; Donaldson T.J., Sadovy Y.2001, Threatened fishes of the world: Cheilinus undulates Ruppell, 1835 (Labridae).Environ.Biol.Fish.62:428), In recent years, people have only begun to pay attention to the artificial breeding, breeding, and genetic analysis of this species. A small wound healing hyperplasia tissue at the edge of the fin rays was taken, treated with colchicine, hypotonic with KCL solution, fixed with Carnoy's solution, and made into hot drop slides. The metaphase chromosomes prepared had good morphology and a large number of cl...

Embodiment 2

[0029] In June 2016, two C. chinensis with well-developed gonads were collected from the Rizhao Fisheries Research Institute. The length of the male shell is about 17.8 cm, and the weight is 273.17 g. The length of the female shell is 19.5 cm, and the weight is 291.31 g. The distribution of C. chinensis is widespread in my country, almost all of the coastal provinces in my country. The meat is delicious, nutritious, and of high economic value, but there are relatively few studies on C. There are not many research reports on breeding conditions. Due to the particularity of its reproductive biology, the production of artificial seedlings has not yet reached the level of large-scale production. In recent years, the growing market demand has led to overfishing and overharvesting, and the natural resources of C. , the conservation and genetic diversity research of P. chinensis needs to be strengthened urgently. After making a tiny wound directly on the edge of the gills, the wound he...

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Abstract

The invention provides a method for rapidly preparing metaphase chromosomes of marine valuable and rare fishes or shellfishes, and belongs to the technical field of marine organisms. The method comprises the steps that 1, test fishes or shellfishes are temporarily cultured, and glass slides are cleaned; 2, after it is guaranteed that the survival state of the test animals is good, the fishes or shellfishes of which fin rays or grills have wounds are selected to be independently cultured; 3, healing and proliferating tissue at the wounds of the test animals is taken to be subjected to chromosome preparation. The method for preparing the metaphase chromosomes is simple and rapid and convenient to operate, the vigorous division and proliferation capacity of the healing and proliferating tissue is fully utilized, it is basically guaranteed that the test animals still can be healthy and be survived after chromosome preparation, multiple times of preparation can be repeated, the needed test animal can be least to one individual, and the method is suitable for various marine valuable and rare fishes and shellfishes. According to the method, freshness of the experiment samples can be guaranteed, the cell activity is good, the defects that most of the valuable and rare fishes or shellfishes are in a mature or aged state, and cell division is not vigorous are overcome, the prepared metaphase chromosomes are good in morphology and dispersion degree, and therefore observation and counting are convenient.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a method for rapidly preparing metaphase chromosomes of marine rare fish or shellfish. Background technique [0002] Chromosomes are carriers of genetic material. Studying species chromosomes can not only explore their taxonomic status and phylogenetic evolution, but also contribute to the identification and community analysis of closely related species, which is of great significance to the development and utilization of resources and genetic breeding. The ocean is a treasure trove of biodiversity. Marine biological resources have potential or actual value. They are an important source of food for human beings and provide 22% of animal protein for human beings in the world every year. Many marine animals also have important medicinal and industrial values. Among the 24 phyla recorded in the animal kingdom, there are more than 2,500 species in 3 phyla such as m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q1/6841C12Q2563/107
Inventor 周丽青杨爱国刘志鸿吴彪孙秀俊陈四清
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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