A method for creating high-quality powdery mildew resistant breeding elements based on tufted wheat
A powdery mildew resistance and powdery mildew resistance gene technology is applied in the field of creation of high-quality powdery mildew resistance breeding elements based on trichomes, which can solve problems such as poor germplasm resources of common wheat and narrow genetic basis of wheat
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Embodiment 1
[0022] A method for creating a high-quality powdery mildew resistance breeding element based on tufted wheat, comprising the following steps: as figure 1 as shown,
[0023] (1) According to the requirements of the breeding objectives, the apical small segment translocation line Nj1 carrying the powdery mildew resistance gene Pm21 of the velvet wheat bred by the Institute of Cytogenetics of Nanjing Agricultural University was selected as the female parent, and the nationally approved high-quality strong gluten bred by the Zhenjiang Academy of Agricultural Sciences The red-skinned wheat Zhenmai No. 168 was used as the male parent, and the two were sexually crossed to obtain F 1 ;
[0024] (2) Hybrid F 1 Firstly backcross 6 consecutive times with the small fragment translocation line Nj1, and then self-cross once to maintain the excellent resistance of the recurrent parent, break gene linkage, expand gene recombination, and obtain BC 5 f 2 group;
[0025] (3) Use root tip ce...
Embodiment 2
[0028] It is basically the same as Example 1, except that the small fragment insertion translocation line Nj2 bred by the Institute of Cytogenetics of Nanjing Agricultural University and carrying the powdery mildew resistance gene Pm21 of pilosa wheat was selected as the female parent. High-quality medium-gluten wheat Zhenmai No. 8 was used as the male parent.
Embodiment 3
[0030] It is basically the same as Example 1, except that the small fragment insertion translocation line Nj2 bred by the Institute of Cytogenetics of Nanjing Agricultural University and carrying the powdery mildew resistance gene Pm21 of pilosa wheat was selected as the female parent. Zhenmai No. 168, a high-quality strong-gluten red-skinned wheat, was used as the male parent.
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