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Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element

A technology for powdery mildew resistance and powdery mildew resistance gene is applied in the field of creating high-quality powdery mildew-resistant breeding elements based on tufted wheat, and can solve the problems of poor germplasm resources of common wheat and narrow wheat genetic base.

Active Publication Date: 2015-12-16
赫聪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the intensification and modernization of wheat production, the genetic basis of cultivated wheat has become increasingly narrow, and the environmental and biological stresses have become more serious. The lack of germplasm resources has become the main bottleneck for breakthroughs in common wheat breeding.

Method used

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  • Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element
  • Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element
  • Creation method for dasypyrum villosum-based high-quality powdery mildew-resistant breeding element

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for creating a high-quality powdery mildew resistance breeding element based on tufted wheat, comprising the following steps: as figure 1 as shown,

[0023] (1) According to the requirements of the breeding objectives, the apical small segment translocation line Nj1 carrying the powdery mildew resistance gene Pm21 of the velvet wheat bred by the Institute of Cytogenetics of Nanjing Agricultural University was selected as the female parent, and the nationally approved high-quality strong gluten bred by the Zhenjiang Academy of Agricultural Sciences The red-skinned wheat Zhenmai No. 168 was used as the male parent, and the two were sexually crossed to obtain F 1 ;

[0024] (2) Hybrid F 1 Firstly backcross 6 consecutive times with the small fragment translocation line Nj1, and then self-cross once to maintain the excellent resistance of the recurrent parent, break gene linkage, expand gene recombination, and obtain BC 5 f 2 group;

[0025] (3) Use root tip ce...

Embodiment 2

[0028] It is basically the same as Example 1, except that the small fragment insertion translocation line Nj2 bred by the Institute of Cytogenetics of Nanjing Agricultural University and carrying the powdery mildew resistance gene Pm21 of pilosa wheat was selected as the female parent. High-quality medium-gluten wheat Zhenmai No. 8 was used as the male parent.

Embodiment 3

[0030] It is basically the same as Example 1, except that the translocation line Nj2 of the small fragment carrying the powdery mildew resistance gene Pm21 of tufted wheat bred by the Institute of Cytogenetics of Nanjing Agricultural University was selected as the female parent. Zhenmai No. 168, a high-quality strong-gluten red-skinned wheat, was used as the male parent.

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Abstract

The invention discloses a creation method for a dasypyrum villosum-based high-quality powdery mildew-resistant breeding element. By backcrossing a top small fragment translocation line Nj1 or a small fragment intercalary translocation line Nj2 carrying the powdery mildew-resistant pm21 gene of dasypyrum villosum with a powdery mildew-infected wheat variety, Zhenmai No. 168 or Zhenmai No. 8, for multiple times and simultaneously screening a homozygous alien chromosome family from backcross descendants in combination with the genomic in-situ hybridization (GISH) of metaphase chromosomes of root-tip cell mitosis, a small fragment translocation homozygous plant which is uniform in agronomic traits and cytogenetically stable and keeps excellent powdery mildew resistance is obtained in order for breeders to improve wheat varieties.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and in particular relates to a method for creating high-quality powdery mildew-resistant breeding elements based on trichomes. Background technique [0002] Wheat is one of the food crops with the widest distribution, the largest planting area, the second largest output, the largest trade volume, and the highest nutritional value in the world. The main wheat producing countries have always attached great importance to the improvement of wheat varieties. However, with the intensification and modernization of wheat production, the genetic basis of cultivated wheat has become increasingly narrow, and the environmental and biological stresses have become more serious. The lack of germplasm resources has become the main bottleneck for breakthroughs in common wheat breeding. Wild relatives of wheat contain abundant genetic resources. At present, many genes related to quality, disease resistance...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 温明星
Owner 赫聪
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