Method for rapidly obtaining herba erigernotis triploid
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A technology of scutellaria and triploids, applied in the field of plant genetics and breeding, can solve the problems of low frequency of occurrence and inability to meet the needs of breeding and utilization
Active Publication Date: 2014-03-26
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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Due to the low frequency of natural occurrence of triploid scutellaria, which cannot meet the needs of breeding and utilization, it is necessary to establish a set of technical methods for artificially creating triploid scutellaria, so as to provide rich and different genetic resources for the breeding and breeding of new triploid varieties. New Triploid Germplasm for Background
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Embodiment 1
[0019] Select 1,000 plump seeds of Erigeron breviscapus harvested in the same year.
[0020] After adding an appropriate amount of distilled water for pre-culture for 16 hours, soak in 50 mL of Oryzalin aqueous solution with a concentration of 60 μmol / L for 24 hours. After the treatment, wash with tap water 3 times.
[0021] Mix the treated seeds with river sand, spread them, cover them with fine soil with a thickness of about 2 mm, spray water to moisten them, and then carry out normal seedling management.
[0022] When the seed emerges with 4-6 true leaves, tear off the lower epidermis of the fourth true leaf for stomatal identification. The specific method is as follows: measure the length and width of the stomata under a microscope, and calculate the perimeter of the stomata according to the ellipse perimeter formula, and measure 30-50 stomata for each plant. Statistical T-test method was used to determine whether the stomatal perimeter of the plant was different from...
Embodiment 2
[0027] Select 1,000 plump seeds of Erigeron breviscapus harvested in the same year.
[0028] After adding an appropriate amount of distilled water for pre-culture for 16 hours, soak in 50 mL of Oryzalin (Oryzalin) aqueous solution with a concentration of 90 μmol / L for 72 hours. After the treatment, wash with tap water 3 times.
[0029] Mix the seeds with river sand, spread them, cover them with fine soil about 2mm thick, spray them with water to moisten them, and then carry out normal seedling management.
[0030] When the seed emerges with 4-6 true leaves, tear off the lower epidermis of the fourth true leaf for stomatal identification. The specific method is as follows: measure the length and width of the stomata under a microscope, and calculate the perimeter of the stomata according to the ellipse perimeter formula, and measure 30-50 stomata for each plant. Statistical T-test method was used to determine whether the stomatal perimeter of the plant was different from t...
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Abstract
The invention discloses a method for rapidly obtaining herba erigernotis triploid. The method comprises the following steps: processing plump herba erigernotis seeds picked in the same year with a water solution of a cell microtubule inhibitor Oryzalin to induce the chromosome doubling of the germ growing point cells; continuously carrying out two times of leaf air hole identification in the seedling stage and the squaring stage, carrying out rapid screening so as to obtain tetraploid variant plants; and hybridizing the tetraploid variant plants with diploid plants (namely fusing egg cells having doubled chromosome with normal sperm cells) so as to obtain ploidy-homozygous triploid. The method utilizes the fusion of single cells (egg cells) with single cells (sperm cells) during the fertilization process of sexual reproduction of angiosperm, simultaneously achieves the ploidy purification of plant chromosome and obtaining of triploid, avoids a large amount of tedious work on purifying the ploidy of the tetraploid variant plants, and provides technical support for cultivating abundant herba erigernotis triploid novel germplasm and breeding triploid novel species.
Description
technical field [0001] The invention belongs to the field of plant genetics and breeding, and relates to a method for cultivating triploid plants, in particular to a method for quickly obtaining triploid scutellaria. Background technique [0002] Triploidy plays an important role in plant breeding. Utilizing the hugeness brought about by the increase in the size of triploid cells, the dosage effect brought about by the doubling of the chromosome set, and the infertility brought about by abnormal meiosis, it is possible to breed high-yielding, active ingredient content, seedless or few-seeded plants New varieties of plants. At present, there are reports of excellent triploid varieties on some plants, such as Luo Han Guo (Li Feng, Jiang Xiangjun, Jiang Shuiyuan, Qin Jisheng, Huang Xiyang, Liu Fengying. Successful cultivation of seedless (less seed) Luo Han Guo (briefings), Guangxi Plants, 2008 ,28(6):27), watermelon (Li Jianwu, Ma Changsheng, Sun Zhongwei, Zhang Jie. A new s...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/08
Inventor 董志渊李绍平杨丽英王馨李林玉杨斌马维思严世武
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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