Dripping piece preparation method of plant chromosome
A chromosome and plant technology, which is applied to the field of plant chromosome drop piece preparation, can solve the problems of poor chromosome dispersion effect, increase the number of chromosomes in the metaphase, unfavorable chromosome observation, etc., and achieves easy separation of chromosomes or cells, saving processing time, and processing process. Simple and fast effects
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Embodiment 1
[0030] Example 1 Chromosome drip preparation for DP17 corn variety:
[0031] (1) Put the DP17 corn seeds in a disk filled with fine sand, put them in an artificial climate box, and cultivate at 28°C. When the root grows to 1.5 cm, cut the root tip and put it into a 5 mL centrifuge tube;
[0032] (2) Cut 1.5cm root and put it in ddH 2 O double-distilled water to wash, moisten the roots and put them into a 0.5 mL EP tube (keep the lid open), let the wet roots stick to the tube wall, but the water cannot flow down in strands;
[0033] (3) Put the EP tube into the nitrous oxide (N 2 O) Treat in the treatment room of the treatment device for 2 hours; the nitrous oxide treatment device decompresses the nitrous oxide in the nitrous oxide tank and passes it into the treatment room, so that the N 2 The pressure of O was 0.5 atmospheres, and the root tips were placed in the treatment chamber at 0.5 atmospheres for 2 h. The purpose was to perform pretreatment, using nitrous gas to des...
Embodiment 2
[0043] Example 2 Chromosome drip preparation for DP46 corn variety:
[0044] (1) Put the DP46 corn seeds in a disk filled with fine sand, put them in an artificial climate box, and cultivate at 28°C. When the root grows to 1.5 cm, cut the root tip and put it into a 5 mL centrifuge tube;
[0045] (2) Cut 1.5cm root and put it in ddH 2 O double-distilled water to wash, moisten the roots and put them into a 0.5 mL EP tube (keep the lid open), let the wet roots stick to the tube wall, but the water cannot flow down in strands;
[0046] (3) Put the EP tube into the nitrous oxide (N 2 O) Treat in the treatment room of the treatment device for 3 hours; the nitrous oxide treatment device decompresses the nitrous oxide in the nitrous oxide tank and then passes it into the treatment room, so that the N 2 The pressure of O is 1 atmosphere;
[0047] (4) Add 90% acetic acid aqueous solution to the EP tube and fix for 2.5 h;
[0048] (5) Remove the roots from the acetic acid and use dd...
Embodiment 3
[0056] Example 3 Preparation of chromosome droplets for DP47 corn variety:
[0057] (1) Put the DP47 corn seeds in a disk filled with fine sand, put them in an artificial climate box, and cultivate at 28°C. When the root grows to 1.5 cm, cut the root tip and put it into a 5 ml centrifuge tube;
[0058] (2) Cut 1.5cm root and put it in ddH 2 O double-distilled water to wash, moisten the roots and put them into a 0.5 mL EP tube (keep the lid open), let the wet roots stick to the tube wall, but the water cannot flow down in strands;
[0059] (3) Put the EP tube into the nitrous oxide (N 2 O) Treat in the treatment room of the treatment device for 3 hours; the nitrous oxide treatment device decompresses the nitrous oxide in the nitrous oxide tank and then passes it into the treatment room, so that the N 2 The pressure of O is 0.75 atmospheres;
[0060] (4) Add 90% acetic acid aqueous solution to the EP tube and fix for 3 h;
[0061] (5) Remove the roots from the acetic acid a...
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