Dripping piece preparation method of plant chromosome

A chromosome and plant technology, which is applied to the field of plant chromosome drop piece preparation, can solve the problems of poor chromosome dispersion effect, increase the number of chromosomes in the metaphase, unfavorable chromosome observation, etc., and achieves easy separation of chromosomes or cells, saving processing time, and processing process. Simple and fast effects
CN106350593AInactive Publication Date: 2017-01-25YANGTZE NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGTZE NORMAL UNIVERSITY
Publication Date
2017-01-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a dripping piece preparation method of plant chromosome. The dripping piece preparation method includes steps of placing a plant organ in nitrous oxide gas for pretreatment, and then fixing the plant organ after pretreatment by stationary liquid, and performing enzymolysis on a cell wall of the plant organ by enzyme, and breaking the plant organ after enzymolysis, and preparing the cell suspension liquid; dripping the cell suspension liquid on a glass slide or a cover glass; forming a plant chromosome specimen. The reagent applied to the method is low in toxicity; the crack obtained through treatment is dispersed in a circular or ellipse form by comparing with regular piece making method, the distribution is uniform, and chromosome intersection is few, and dispersing degree is good; a complete chromosome can be obtained; the preparation method can prepare the chromosome slice with stable quality, clear chromosome and good dispersity; the prepared chromosome slice can be applied to cytological identification, manufacturing of permanent slice, hybridization in-situ, microdissection, and microscope separation, and so on.
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Description

technical field

[0001] The invention belongs to the technical field of chromosome preparation, and in particular relates to a method for preparing drop slices of plant chromosomes. Background technique

[0002] Chromosome preparation is the basis for observation of chromosome morphology, in situ hybridization and chromosome separation. The commonly used methods of chromosome preparation mainly include compression method and smear method. However, the tableting method and the smearing method mainly have the following disadvantages: (1) The experimental drugs are highly toxic. The tableting method and smearing method generally use α-bromine, 8-hydroxyquinoline and colchicine as pretreatment solutions, and the drugs are highly carcinogenic; (2) The treatment time is relatively long, and the general pretreatment needs 3-5 hours, fixed processing 1-2 days, it generally takes 1.5-2.5 days from material collection to production, and the processing time is long. Moreover, it is m...

Claims

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