Plant chromosome tablet observation method
A chromosome and tablet technology, applied in the field of chromosome observation, can solve the problems of insignificant color difference and unsatisfactory observation effect
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Embodiment 7
[0026] Embodiment 7: Microscopic examination.
[0027] Observed under a microscope, good slides can be sealed with a mounting medium for long-term storage.
[0028]
[0029] The above-mentioned improved carbo fuchsin staining solution can be prepared by the following method:
[0030] ◆Stock solution preparation:
[0031] A stock solution: Weigh 3g of basic fuchsin and dissolve in 100ml of 70% alcohol
[0032] B stock solution: take 10ml A stock solution and add 90ml 5% carbolic acid aqueous solution
[0033] C stock solution: take 55ml of B stock solution, add 6ml each of glacial acetic acid and formalin (37% formaldehyde)
[0034] ※ The stock solutions of A and C can be stored for a long time, and the stock solution of B is valid within two weeks
[0035] ◆Preparation of staining solution: Take 10-20ml of C stock solution, add 80-90ml of 45% acetic acid and 1.8g of sorbitol to make carbolic fuchsin solution with a concentration of 10-20%. use.
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