Plant chromosome tablet observation method

A chromosome and tablet technology, applied in the field of chromosome observation, can solve the problems of insignificant color difference and unsatisfactory observation effect

Inactive Publication Date: 2015-02-25
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing pressing method, the color difference between chromosomes and other organe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 7

[0026] Embodiment 7: Microscopic examination.

[0027] Observed under a microscope, good slides can be sealed with a mounting medium for long-term storage.

[0028]

[0029] The above-mentioned improved carbo fuchsin staining solution can be prepared by the following method:

[0030] ◆Stock solution preparation:

[0031] A stock solution: Weigh 3g of basic fuchsin and dissolve in 100ml of 70% alcohol

[0032] B stock solution: take 10ml A stock solution and add 90ml 5% carbolic acid aqueous solution

[0033] C stock solution: take 55ml of B stock solution, add 6ml each of glacial acetic acid and formalin (37% formaldehyde)

[0034] ※ The stock solutions of A and C can be stored for a long time, and the stock solution of B is valid within two weeks

[0035] ◆Preparation of staining solution: Take 10-20ml of C stock solution, add 80-90ml of 45% acetic acid and 1.8g of sorbitol to make carbolic fuchsin solution with a concentration of 10-20%. use.

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PUM

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Abstract

The invention discloses a technology for observing a plant chromosome in a tabletting method. The method comprises the steps of preprocessing a testing material subjected to active cell division, fixing the chromosome, carrying out acid digestion on cells, neutralizing with alkali, dyeing, and observing tablets. 1cm to 2cm of a part, subjected to the active cell division, of the test material is preprocessed in a processing solution under a dark condition, after a preprocessed sample is well fixed by a fixed solution, the walls of the cells are subjected to acid digestion by high-concentration hydrochloric acid, the surplus hydrochloric acid is neutralized by weak protoplast base, then the dyeing and tabletting are carried out, and finally the division situation of the chromosome is observed under a microscope.

Description

technical field [0001] The invention relates to a method for observing chromosomes, in particular to a method for observing chromosomes derived from plant test materials by using tablet technology. Background technique [0002] The mitosis of plant meristematic cells has a peak of mitosis every day. At this time, the test material is fixed, stained and pressed, and then placed under a microscope for observation. A large number of cells and chromosomes in various stages of mitosis can be seen. Chromosome compression method is the most commonly used method to observe plant chromosomes, and it is also the basis for studying karyotype, chromosome banding, chromosome aberration and sister chromatid exchange. Combined with modern biotechnology, FISH can clearly determine the position and distribution of the target gene on the chromosome, which is the basis for further in-depth research. In the existing pressing method, the color difference between the chromosome and other organel...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 何平李林光李慧峰王海波
Owner SHANDONG INST OF POMOLOGY
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